Overproduction and one-step purification of the N5,N10-methenyltetrahydromethanopterin cyclohydrolase (Mch) from the hyperthermophilic Methanopyrus kandleri

Extremophiles

Volumn 2, Issue 1, 1998, Pages 15-22

  Vaupel, Martin ^a   Vorholt, Julia A ^a   Thauer, Rudolf K ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Hyperthermophilic enzymes; Methanogenic Archaea; Methanopterin dependent enzymes; Methanopyrus kandleri; N5,N10 Methenyltetrahydromethanopterin cyclohydrolase

Indexed keywords

CHROMATOGRAPHY; ENZYME PURIFICATION; METHENYLTETRAHYDROMETHANOPTERIN CYCLOHYDROLASE;

AMINO ACID SEQUENCE; AMINOHYDROLASES; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ESCHERICHIA COLI; EURYARCHAEOTA; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION, GENETIC;

ARCHAEA; ESCHERICHIA COLI; METHANOBACTERIUM; METHANOCALDOCOCCUS JANNASCHII; METHANOCOCCUS; METHANOPYRUS KANDLERI; METHANOSARCINA BARKERI; METHANOTHERMOBACTER THERMAUTOTROPHICUS;

EID: 0031712856     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007920050038     Document Type: Article

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