Quantitation of the mass of fatty acid ethyl esters synthesized by Hep G2 cells incubated with ethanol

Alcoholism: Clinical and Experimental Research

Volumn 22, Issue 5, 1998, Pages 1125-1131

  Dan, Li ^a   Cluette Brown, Joanne E ^a   Kabakibi, Ayman ^a   Laposata, Michael ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Ethanol; Fatty Acid; Fatty Acid Ethyl Ester; Hep G2 Cells

Indexed keywords

FATTY ACID ESTER;

ALCOHOL CONSUMPTION; ARTICLE; CELL STRAIN HEPG2; CELL TYPE; DOSE RESPONSE; ENZYME SYNTHESIS; FATTY ACID SYNTHESIS; HUMAN; HUMAN CELL; INTOXICATION; MEDIATOR RELEASE; PRIORITY JOURNAL;

EID: 0031712588     PISSN: 01456008     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1530-0277.1998.tb03711.x     Document Type: Article

References (24)

1. Newsome WH, Rattray JBM: The enzymatic esterification of ethanol with fatty acids. Can J Biochem 43:1223-1233, 1965
2. Patton S, McCarthy RD: Conversion of alcohol to ethyl esters of fatty acids by the lactating goat. Nature 209:616-617, 1966
3. Lange LG, Bergmann SR, Sobel BE: Identification of fatty acid ethyl esters as products of rabbit myocardial ethanol metabolism. J Biol Chem 256:12968-12973, 1981
4. Laposata EA, Lange LG: Presence of nonoxidative ethanol metabolism in human organs commonly damaged by ethanol abuse. Science 231:497-499, 1986
5. Lieber CS, DeCarli LM: Ethanol oxidation by hepatic microsomes; adaptive increase after ethanol feeding. Science 162:917-918, 1968
6. Lochner A, Cowley R, Brink AJ: Effect of ethanol on metabolism and function of perfused rat heart. Am Heart J 78:770-780, 1969
7. Raskin NH, Sokoloff L: Enzymes catalyzing ethanol metabolism in neural and somatic tissues of the rat. J Neurochem 19:273-282, 1972
8. Hamamoto T, Yamada S, Hirayama C: Nonoxidative metabolism of ethanol in the pancreas; implication in alcoholic pancreatic damage. Biochem Pharmacol 39:241-245, 1990
9. Lange LG, Sobel BE: Mitochondial dysfunction induced by fatty acid ethyl esters, myocardial metabolites of ethanol. J Clin Invest 72:724-731, 1983
10. Haber PS, Wilson JS, Apte MV, Pirola RC: Fatty acid ethyl esters increase rat pancreatic lysosomal fragility. J Lab Clin Med 121:759-64, 1993
11. Szczepiorkowski ZM, Dickersin GR, Laposata M: Fatty acid ethyl esters decrease human hepatoblastoma cell proliferation and protein synthesis. Gastroenterology 108:515-522, 1995
12. Werner J, Laposata M, Castillo CF, Saghir M, Iozzo RV, Lewandrowski KB, Warshaw AL: Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of ethanol. Gastroenterology 113:286-294, 1997
13. Furth EE, Sprecher H, Fisher EA, Fleishman HD, Laposata M: An in vitro model for essential fatty acid deficiency: HepG2 cells permanently maintained in lipid-free medium. J Lipid Res 33:1719-1726, 1992
14. Capriotti AM, Laposata M: Identification of variables critical to reproducible delipidation of serum. J Tissue Cult Methods 10:219-222, 1986
15. Bernhardt TG, Cannistraro PA, Bird DA, Doyle KM, Laposata M: Purification of fatty acid ethyl esters by solid phase extraction and HPLC. J Chromatogr B 675:189-196;1996
16. Laposata M, Szczepiorkowski ZM, Brown JE: Fatty acid ethyl esters: nonoxidative metabolites of ethanol. Prostaglandins Leukot Essent Fatty Acids 52:87-91, 1995
17. Hallaq Y, Becker TC, Manno CS, Laposata M: Use of acetyl chloride/methanol for assumed selective methylation of plasma nonesterified fatty acids results in significant methylation of esterified fatty acids. Lipids 28:355-360, 1993
18. Clemens DL, Halgard CM, Miles RR, Sorrell MF, Tuma DJ: Establishment of a recombinant hepatic cell line stably expressing alcohol dehydrogenase. Arch Biochem Biophys 321:311-318, 1995
19. Laposata M, Prescott SM, Bross TE, Majerus PW: Development and characterization of a tissue culture cell line with essential fatty acid defiency. Proc Natl Acad Sci USA 79:7654-7658, 1982
20. Laposata M, Kaiser SL, Capriotti AM: Icosanoid production can be decreased without alterations in cellular arachidonate content or enzyme activities required for arachidonate release and icosanoid synthesis. J Biol Chem 263:3266-3273, 1988
21. Capriotti AM, Furth EE, Arrasmith ME, Laposata M: Arachidonate released upon agonist stimulation preferentially originates from arachidonate most recently incorporated into nuclear membrane phospholipids. J Biol Chem 263:10029-10034, 1988
22. Tsujita T, Okuda H: Fatty acid ethyl ester-synthesizing activity of lipoprotein lipase from rat postheparin plasma. J Biol Chem 269:5884-5889, 1994
23. Riley DJS, Kyger EM, Spilburg CA, Lange LG: Pancreatic cholesterol esterase. 2. Purification and characterization of human pancreatic fatty acid ethyl ester synthase. Biochemistry 29:3848-3852, 1990
24. Tsujita T, Okuda H: The synthesis of fatty acid ethyl ester by carboxylester lipase. Eur J Biochem 224:57-62, 1994