Ethionine toxicity in vitro: The correlation of data from rat hepatocyte suspensions and monolayers with in vivo observations

Archives of Toxicology

Volumn 72, Issue 9, 1998, Pages 588-596

  Waterfield, Catherine J ^a   Westmoreland, Carl ^b   Asker, Daniel S ^a   Murdock, Janice C ^b   George, Elisabeth ^b   Timbrell, John A ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Ethionine; Hepatocytes; In vivo in vitro correlations

Indexed keywords

ANIMAL CELL; ARTICLE; CYTOPATHOGENIC EFFECT; DRUG EFFECT; FATTY ACID OXIDATION; FEMALE; LIVER CELL; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS REGULATION; RAT; TOXICITY TESTING;

ADENOSINE TRIPHOSPHATE; ALANINE TRANSAMINASE; ANIMALS; ASPARTATE AMINOTRANSFERASES; CELLS, CULTURED; CITRULLINE; ETHIONINE; FATTY ACIDS; FEMALE; GLUTATHIONE; L-LACTATE DEHYDROGENASE; LIVER; MALE; OXIDATION-REDUCTION; PROTEIN BIOSYNTHESIS; RATS; TOXICITY TESTS; TRIGLYCERIDES; UREA;

ANIMALIA;

EID: 0031712302     PISSN: 03405761     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002040050547     Document Type: Article

References (45)

1. Bacon JA, Linseman DA (1996) Mechanistic differences in ethionine toxicity between Chang or HepG2 liver cell lines and primary hepatocytes. In Vitro Toxicol 9: 56-60
2. Bergmeyer H, Bernt E, Hess B (1965) Lactate dehydrogenase (II 2a). In: Bergmeyer H (ed) Methods of enzymatic analysis. Academic Press, New York, pp 736-743
3. Berry MN, Edwards AM, Barritt GJ (1991) Isolated hepatocytes preparation, properties and applications. Elsevier, Amsterdam
4. Blaauboer BJ, Boobis AR, Castell JV, Hawksworth GM, Lorenzon G, Miltenburger HG, Rogiers V, Skett P, Villa P, Wiebel FJ (1994) The practical applicability of hepatocyte cultures in routine testing. ATLA 22: 231-241
5. Borenfreund E, Puerner JA (1985) Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett 24: 119-124
6. Brindle NP, Ontko JA (1986) Suppression of triglyceride secretion by epinephrine in isolated rat hepatocytes. Biochem Biophys Res Commun 141: 191-197
7. Crocker CL (1967) Rapid determination of urea nitrogen in serum or plasma without deproteinization. Am J Med Technol 33: 361
8. Deboyser D, Goethals F, Krack G, Roberfroid M (1989) Investigation into the mechanism of tetracycline-induced steatosis: study in isolated hepatocytes. Toxicol Appl Pharmacol 473-479
9. Deboyser D, Goethals F, Roberfroid M (1992) Biochemical effects of methotrexate in isolated hepatocytes in relation to its steatogenic activity. Toxicol In Vitro 6: 129-132
10. Delaney J, Timbrell JA (1995) Role of cytochrome P450 in hydrazine toxicity in isolated hepatocytes in vitro. Xenobiotica 25: 1399-1410
11. De Master EG, Redfern B (1987) High performance liquid chromatography of hepatic thiols with electrochemical detection. Methods Enzymol 142: 110-114
12. Farber E (1967) Ethionine fatty liver. Adv Lipid Res 5: 119-183
13. Folch J, Lees M, Sloane, Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 224: 497-509
14. Fry JR, Hammond AH, Garle MJ, Lal K (1993) Comparison of xenobiotic-medialed cytotoxicity in rat cultured hepatocytes and the V79 Chinese hamster lung fibroblast cell line: can metabolically-activated hepatotoxins be identified by selective cytotoxicity to hepatocytes? ATLA 21: 8-12
15. George E, Hamilton G, Westmoreland C (1996) The use of in vitro models in hepatotoxicity testing. TEN 3: 142-152
16. Girard J, Malewiak M-I (1986) Fatty acid oxidation and ketogenesis in isolated hepatocytes. In: Guillouzo A, Guguen-Guillouzo C (eds) Research in isolated and cultured hepatocytes. John Libby, UK pp 87-112
17. Glaser G, Mager J (1974) Biochemical studies on the mechanism of action of liver poisons. III Depeletion of glutathione in ethionine poisoning. Biochim Biophys Acta 372: 237-244
18. Goethals F, Krack G, Deboyser D, Vossen P, Roberfroid M (1984) Critical biochemical functions of isolated hepatocytes as sensitive indicators of chemical toxicity. Fundam Appl Toxicol 4: 441-450
19. Gravela E, Poli G, Albano E, Dianzani MU (1977) Studies on fatty liver with isolated hepatocytes. I. The action of colchicine, phalloidin, cytochalasin B and sysloheximide on protein and triglyceride synthesis and secretion. Exp Mol Pathol 27: 339-352
20. Gravela E, Albano E, Dianzani MU, Poli G, Slater TF (1979) Effects of carbon tetrachloride on isolated hepatocytes. Biochem J 178: 509-512
21. Guguen-Guillouzo C, Gripon P, Vandenberghe Y, Lamballe F, Ratanasavanh D, Guillouzo A (1988) Hepatotoxicity and molecular aspects of hepatocyte function in primary culture. Xenobiotica 18: 773-783
22. Guillouzo A (1992) Hepatotoxicity. In: Frazier JM (ed) In vitro toxicity testing. Marcel Dekker, New York, pp 45-83
23. Hissin PJ, Hilf R (1976) A fluorimetric method for determination of oxidised and reduced glutathione in tissues. Anal Biochem 74: 214-226
24. Katoh NK, Shimbayashi K, Abe K, Sakurada K (1991) Decreased estradiol receptor concentrations in ethionine-induced fatty liver of rats. Toxicol Lett 58: 279-285
25. Lyon AW, Kisilevsky R (1990) Inhibition of the initiation of hepatic protein synthesis during ethionine mediated ATP depletion in vivo: modification to ribosomal subunits, evidence of impaired ternary complex formation and a subcellular redistribution of eIF-2 alpha. Biochim Biophys Acta 1049: 158-170
26. McGowen MW, Artiss JD, Stranbergh DR, Zak B (1983) Peroxidase coupled methods for the colourimetric determination of serum triglycerides. Clin Chem 29: 538
27. McLean P (1966) Effect of ethionine treatment on the activity of enzymes concerned with urea synthesis in rat liver. Biochem J 99: 776-779
28. Moldeus P, Hogberg J, Orrenius S (1978) Isolation and use of liver cells. Methods Ezymol 52: 60-71
29. Mori M (1983) Ultrastructural changes of hepatocyte organelles induced by chemicals and their relation to fat accumulation in the liver. Acta Pathol Jpn 33: 922-922
30. Rogiers V, Blaauboer B, Maurel P, Phillips IR, Shephard E (1995) Hepatocyte-based in vitro models and their application in pharmacotoxicology. Toxicol In Vitro 9: 685-694
31. Seglen PO (1978) Effects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes. Biochem J 174: 469-474
32. Stanley PE, Williams SG (1969) Use of the liquid scintillation spectrometer for determining adenosine triphosphate by luciferase enzyme. Anal Biochem 29: 381-392
33. Storey DL, Gee SJ, Tyson CA (1983) Response of isolated hepatocytes to organic and inorganic cytotoxins. J Toxicol Environ Health 11: 483-501
34. Timbrell JA (1986) The liver as a target organ for toxicity. In: Cohen GM (ed) Target organ toxicity CRC Press, Florida, pp 145-173
35. Timbrell JA (1994) Do in vivo and in vitro biochemical and toxic effects correlate? Studies with hydrazine. TEN 1: 131-133
36. Tyson CA, Green CE (1987) Cytotoxicity measures: choices and methods. In: Raukman EJ, Padilla GM (eds) The isolated hepatocyte. Use in toxicology and xenobiotic biotransformations. Academic Press, New York, pp 119-158
37. Villa-Trevino S, Shull SH, Farber E (1963) The role of adenosine triphosphate deficiency in ethionine-induced inhibition of protein synthesis. J Biol Chem 238: 1757-1763
38. Wang C, Strauss L, Paronetto F, Adlersberg D (1958) Effects of ethionine administration in rabbits and dogs. AMA Arch Pathol 65: 286-294
39. Waterfield CJ, Timbrell JA (1996) The biosynthesis of taurine from N-acetyl-L-cysteine and other precursors in vivo and in rat hepatocytes. Amino Acids 10: 173-185
40. Waterfield CJ, Turton JA, Scales MDC, Timbrell JA (1993) Investigations into the effects of various hepatotoxic compounds on urinary and liver taurine levels in rats. Arch Toxicol 67: 244-254
41. Waterfield CJ, Asker DS, Timbrell JA (1996) Does urinary taurine reflect changes in protein metabolism? A study with cycloheximide in rats. Biomarkers 2: 107-114
42. Waterfield CJ, Delaney J, Kerai MDJ, Timbrell JA (1997a) Correlations between in vivo and in vitro effects of toxic compounds: studies with hydrazine. Toxicol In Vitro 11: 217-227
43. Waterfield CJ, Asker DS, Timbrell JA (1997b) Triglyceride disposition in isolated hepatocytes after treatment with hydrazine. Chem Biol Interact 107: 157-172
44. Wu C (1955) Effect of ethionine on the urinary nitrogenous constituents of adult rats. Arch Biochem Biophys 56: 230-234
45. Zimmerman HJ (1978) Hepatotoxicity: the adverse effects of drugs and other chemicals and the liver. Appleton Century-Crofts, New York