Independent regulation of two separate gene activities in a continuous human cell line

Biological Chemistry

Volumn 379, Issue 8-9, 1998, Pages 1189-1191

  Moradpour, Darius ^a   Englert, Christoph ^b   Blum, Hubert E ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

Ecdysone; Regulated gene expression; Tetracycline

Indexed keywords

BETA GALACTOSIDASE; ECDYSONE; LUCIFERASE; TETRACYCLINE;

ARTICLE; CELL LINE; CONTINUOUS CULTURE; CONTROLLED STUDY; EUKARYOTIC CELL; GENE ACTIVITY; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; REPORTER GENE;

CELL LINE; CULTURE MEDIA; ECDYSTERONE; GENE EXPRESSION REGULATION; HUMANS; MICROSCOPY, FLUORESCENCE; TETRACYCLINE;

EUKARYOTA; MAMMALIA;

EID: 0031709954     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1989). Current Protocols in Molecular Biology (New York: John Wiley & Sons).
2. Baker, S.J., Markowitz, S., Fearon, E.R., Willson, J.K.V., and Vogelstein, B. (1990). Suppression of human colorectal carcinoma cell growth by wild-type p53. Science 249, 912-915.
3. Baron, U., Freundlieb, S., Gossen, M., and Bujard, H. (1995). Coregulation of two gene activities by tetracycline via a bidirectional promoter. Nucleic Acids Res. 23, 3605-3606.
4. Englert, C., Hou, X., Maheswaran, S., Bennett, P., Ngwu, C., Re, G.G., Garvin, A.J., Rosner, M.R., and Haber, DA (1995). WT1 suppresses synthesis of the epidermal growth factor receptor and induces apoptosis. EMBO J. 14, 4662-4675.
5. Gossen, M., and Bujard, H. (1992). Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89, 5547-5551.
6. Gossen, M., Freundlieb, S., Bender, G., Müller, G., Hillen, W., and Bujard, H. (1995). Transcriptional activation by tetracyclines in mammalian cells. Science 268, 1766-1769.
7. Moradpour, D., Englert, C., Wakita, T., and Wands, J.R. (1996). Characterization of cell lines allowing tightly regulated expression of hepatitis C virus core protein. Virology 222, 51-63.
8. Morgenstern, J.P., and Land, H. (1990). Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 18, 3587-3596.
9. No, D., Yao, T.-P., and Evans, R.M. (1996). Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc. Natl. Acad. Sci. USA 93, 3346-3351.
10. Precious, B., Young, D.F., Bermingham, A., Fearns, R., Ryan, M., and Randall, R.E. (1995). Inducible expression of the P. V. and NP genes of the paramyxovirus Simian virus 5 in cell lines and an examination of NP-P and NP-V interactions. J. Virol. 69, 8001-8010.
11. Shockett, P.E., and Schatz, D.G. (1996). Diverse strategies for tetracycline-regulated inducible gene expression. Proc. Natl. Acad. Sci. USA 93, 5173-5176.