Determination of serum physiological concentration of methylmalonic acid by gas chromatography-mass spectrometry with selected ion monitoring

Annals of Clinical Biochemistry

Volumn 35, Issue 5, 1998, Pages 633-636

  Rifai, Nader ^b   Hagen, Thilo ^a   Bradley, Loetta ^a   Sakamoto, Masayuki ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Cobalamin deficiency; Inborn errors of metabolism; Vitamin B12

Indexed keywords

METHYLMALONIC ACID;

ARTICLE; BLOOD ANALYSIS; GAS CHROMATOGRAPHY; ION MONITORING; MASS SPECTROMETRY; METHYLMALONIC ACIDEMIA; PHYSIOLOGY; PRIORITY JOURNAL; SOLID PHASE EXTRACTION;

EID: 0031709953     PISSN: 00045632     EISSN: None     Source Type: Journal    
DOI: 10.1177/000456329803500506     Document Type: Article

References (7)

1. Allan RH, Stabler SP, Savage DG, Lindenbaum J. Diagnosis of cobalamin deficiency. I: usefulness of serum methylmalonic acid and total homocysteine concentrations. Am J Hematol 1990; 34: 90-8
2. Fenton WA, Rosenberg LE. Disorders of propionate and methylmalonate metabolism. In: Scriver CR, Beaudet AL, Sly WS, Valle D, eds. The Metabolic and Molecular Bases of Inherited Disease, 7th edition. New York; McGraw Hill, 1995: 1423-50
3. Marcell PD, Stabler SP, Podell ER, Allen RH. Quantitation of methylmalonic acid and other dicarboxylic acids in normal serum and urine using capillary gas-chromatography mass spectrometry. Anal Biochem 1985; 150: 58-66
4. Hoffmann G, Aramaki S, Blum-Hoffmann E, Nyhan WL, Sweetman L. Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic mass spectrometric analysis. Clin Chem 1989; 35: 587-95
5. Rasmussen K. Solid-phase sample extraction for rapid determination of methylmalonic acid in serum and urine by a stable-isotope-dilution method. Clin Chem 1989; 35: 260-4
6. Zinn AB, Hine DG, Mahoney MJ, Tanaka K. The stable dilution method for measurement of methylmalonic acid: a highly accurate approach to the prenatal diagnosis of methylmalonic acidemia. Pediatr Res 1982; 16: 740-5
7. Sweetman L. Quantitative GCMS of urinary organic acids and plasma methylmalonic, propionic and isovaleric acids. In: Matsumoto I, Kuhara O, Mamer OA, Sweetman L, Calderhead RG, eds. Advances in Chemical Diagnosis and Treatment of Metabolic Disorders. Tokyo: Kanazawa Medical Publishers Ltd. 1994: 61-72