A simple PCR method for distinguishing Saccharomyces cerevisiae from its sibling species by amplification of the RPL2 region

Journal of Fermentation and Bioengineering

Volumn 86, Issue 3, 1998, Pages 249-252

  Ryu, Seung Lim ^a   Mikata, Kozaburo ^b   Murooka, Yoshikatsu ^a   Kaneko, Yoshinobu ^a  

^a OSAKA UNIVERSITY   (Japan)

^b Institute for Fermentation Osaka   (Japan)

Author keywords

Distinction; PCR; Rapid identification; RPL2; Saccharomyces sensu stricto

Indexed keywords

CELLS; CHROMOSOMES; DNA SEQUENCES; ELECTROPHORESIS; EXTRACTION; FATTY ACIDS; FERMENTATION; FRUCTOSE; GENES; MOLECULES; PROTEINS; YEAST;

POLYMERASE CHAIN REACTION; RAPID IDENTIFICATION; RPL2; SACCHAROMYCES SENSU STRICTO;

ENZYMES;

ARTICLE; CHROMOSOME REARRANGEMENT; DNA DNA HYBRIDIZATION; DNA FLANKING REGION; DNA SEQUENCE; FUNGUS IDENTIFICATION; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; SACCHAROMYCES CEREVISIAE; SIBLING; SPECIES DIFFERENTIATION;

SACCHAROMYCES BAYANUS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES PARADOXUS; SACCHAROMYCES PASTORIANUS;

EID: 0031703821     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0922-338X(98)80001-9     Document Type: Article

References (28)

1. van der Walt, J. P.: The genus Saccharomyces (Meyen) Reess, p.555-718. In Lodder, J. (ed.), The yeasts, a taxonomic study. Elsevier/North-Holland, Amsterdam (1970).
2. Scheda, R. and Yarrow, D.: The instability of physiological properties used as criteria in the taxonomy of yeasts. Arch. Mikrobiol., 55, 209-225 (1966).
3. Scheda, R. and Yarrow, D.: Variation in the fermentation pattern of some Saccharomyces species. Arch. Mikrobiol., 61, 310-316 (1968).
4. Vaughan-Martini, A.: Saccharomyces paradoxus comb, nov., a newly separated species of the Saccharomyces sensu stricto complex based upon nDNA/nDNA homologies. System. Appl. Microbiol., 12, 179-182 (1989).
5. Vaughan-Martini, A. and Kurtzman, C. P.: Deoxyribonucleic acid relatedness among species of the genus Saccharomyces sensu stricto. Int. J. Syst. Bacteriol., 35, 508-511 (1985).
6. Vaughan-Martini, A. and Martini, A.: Three newly delimited species of Saccharomyces sensu stricto. Antonie van Leeuwenhoek, 53, 77-84 (1987).
7. Barnett, J. A.: The taxonomy of the genus Saccharomyces Meyen ex Reess: a short review for non-taxonomists. Yeast, 8, 1-23 (1992).
8. Luwicka, K., Mallie, M., and Bastide, J.-M.: Genetic variability in the Saccharomyces sensu stricto complex revealed by mutilocus enzyme electrophoresis. Int. J. Syst. Bacteriol., 45, 538-543 (1995).
9. Tornai-Lehoczki, J., Dlauchy, D., and Dealt, T.: Significance of active fructose transport in the differentiation of the Saccharomyces sensu stricto group. Lett. Appl. Microbiol., 19, 173-176 (1994).
10. Augustyn, O. P. H., Ferreira, D., and Kock, J. L. F.: Differentiation between yeast species, and strains within a species, by cellular fatty acid analysis. IV. Saccharomyces sensu stricto, Hanseniaspora, Saccharomycodes and Wickerhamiella. System. Appl. Microbiol., 14, 324-334 (1991).
11. Kononova, S. V., Tsiomenko, A. B., and Golubev, W. I.: Extracellular glycoprotein specific for Saccharomyces sensu stricto. FEMS Microbiol. Lett., 113, 77-80 (1993).
12. Kaneko, Y. and Banno, I.: Reexamination of Saccharomyces bayanus strains by DNA-DNA hybridization and electrophoretic karyotyping. Institute for Fermentation Osaka Research Communications, 15, 30-41 (1991).
13. Kishimoto, M., Soma, E., and Goto, S.: Classification of cryophilic wine yeasts based on electrophoretic karyotype, G + C content and DNA similarity. J. Gen. Appl. Microbiol., 40, 83-93 (1994).
14. Naumov, G. I., Naumova, E. S., Lantto, R. A., Louis, E. J., and Korhola, M.: Genetic homology between Saccharomyces cerevisiae and its sibling species S. paradoxus and S. bayanus: electrophoretic karyotypes. Yeast, 8, 599-612 (1992).
15. Naumov, G. I., Naumova, E. S., Hagler, A. N., Mendonça-Hagler, L. C., and Louis, E. J.: A new genetically isolated population of the Saccharomyces sensu stricto complex from Brazil. Antonie van Leeuwenhoek, 67, 351-355 (1995).
16. Naumov, G. I., Naumova, E. S., and Louis, E. J.: Two new genetically isolated populations of the Saccharomyces sensu stricto complex from Japan. J. Gen. Appl. Microbiol., 41, 499-505 (1995).
17. Guillamón, J. M., Barrio, E., Huerta, T., and Querol, A.: Rapid characterization of the Saccharomyces sensu stricto complex according to mitochondrial DNA patterns. Int. J. Syst. Bacteriol., 44, 708-714 (1994).
18. Vezinhet, F., Blondin, B., and Hallet, J. N.: Chromosomal DNA patterns and mitochondrial DNA polymorphism as tools for identification of enological strains of Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol., 32, 568-571 (1990).
19. Molnár, O., Messner, R., Prillinger, H., Stahl, U., and Slavikova, E.: Genotypic identification of Saccharomyces species using random amplified polymorphic DNA analysis. System. Appl. Microbiol., 18, 136-145 (1995).
20. Masneuf, I., Aigle, M., and Dubourdieu, D.: Development of a polymerase chain reaction/restriction fragment length polymorphism method for Saccharomyces cerevisiae and Saccharomyces bayanus identification in ecology. FEMS Microbiol. Lett., 138, 239-244 (1996).
21. Nguyen, H.-V. and Gaillardin, C.: Two subgroups within the Saccharomyces bayanus species evidenced by PCR amplification and restriction polymorphism of the non-transcribed spacer 2 in the ribosomal DNA unit. System. Appl. Microbiol., 20, 286-294 (1997).
22. Ryu, S.-L., Murooka, Y., and Kaneko, Y.: Reciprocal translocation at duplicated RPL2 loci might cause speciation of Saccharomyces bayanus and Saccharomyces cerevisiae. Curr. Genet., 33, 345-351 (1998).
23. Carle, G. F. and Olson, M. V.: An electrophoretic karyotype for yeast. Proc. Natl. Acad. Sci. USA, 82, 3756-3760 (1985).
24. Ryu, S.-L., Murooka, Y., and Kaneko, Y.: Genomic reorganization between two sibling yeast species, Saccharomyces bayanus and Saccharomyces cerevisiae. Yeast, 12, 757-764 (1996).
25. Hoffman, C. S. and Winston, F.: A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene, 57, 267-272 (1987).
26. Herbert, C. J., Dujardin, D., Labouesse, M., and Slonimski, P. P.: Divergence of the mitochondrial leucyl tRNA synthetase genes in two closely related yeasts Saccharomyces cerevisiae and Saccharomyces douglasii: a paradigm of incipient evolution. Mol. Gen. Genet., 213, 297-309 (1988).
27. Adjiri, A., Chanet, R., Mezard, C., and Fabre, F.: Sequence comparison of the ARG4 chromosomal regions from the two related yeasts, Saccharomyces cerevisiae and Saccharomyces douglasii. Yeast, 10, 309-317 (1994).
28. Valente, P., Gouveia, F. C., de Lemos, G. A., Pimentel, D., van Elsas, J. D., Mendonca-Hagler, L. D., and Hagler, A. N.: PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures. FEMS Microbiol. Lett., 137, 253-256 (1996).