Spectral characterization of NAD(P)H fluorescence in intact isolated chloroplasts and leaves: Effect of chlorophyll concentration on reabsorption of blue-green fluorescence

Photosynthesis Research

Volumn 56, Issue 3, 1998, Pages 291-301

  Cerovic, Zoran G ^a   Langrand, Emmanuelle ^a   Latouche, Gwendal ^a   Morales, Fermin ^a   Moya, Ismaël ^a  

^a UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

Blue green fluorescence (BGF); Intact isolated chloroplasts; Photosynthesis; Pisum sativum; Pyridine nucleotides; Spinacia oleracea L

Indexed keywords

EID: 0031697176     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006081209887     Document Type: Article

References (45)

1. Ashruf JF, Coremans JMCC, Bruining HA and Inee C (1995) Increase of cardiac work is associated with decrease of mitochondrial NADH. Am J Physiol-Heart Circ Phys 38: H856-H862
2. Bate-Smith EC (1962) The phenolic constituents of plants and their taxonomic significance. J Linn Soc (Bot) 58: 95-175
3. Bongi G, Palliotti A, Rocchi P, Moya I and Goulas Y (1994) Bluegreen fluorescence excited by UV laser on leaves of different species originates from cutin and issensitive to leaf temperature. Plant Cell Environ 17: 777-780
4. Broglia M (1993) Blue-green laser-induced fluorescence from intact leaves-actinic light sensitivity and subcellular origins Appl Opt 32: 334-338
5. Bruinsma J (1961) A comment on the spectrophotometric determination of chlorophyll. Biochim Biophys Acta 52: 576-578
6. Cerovic ZG, Cheesbrough JK and Walker DA (1987) Photosynthesis by intact isolated-chloroplasts on solid support. Plant Physiol 84: 1249-1251
7. Cerovic ZG, Bergher M, Goulas Y, Tosti S and Moya I (1993) Simultaneous measurement of changes in red and blue fluorescence in illuminated isolated chloroplasts and leaf pieces The contribution of NADPH to the blue fluorescence signal. Photosynth Res 36: 193-204
8. Cerovic ZG, Morales F and Moya I (1994) Time-resolved spectral studies of blue-green fluorescence of leaves, mesophyll and chloroplasts of sugar beet (Beta vulgaris L.). Biochim Biophys Acta 1188: 58-68
9. Chappelle EW, Wood FM, McMurtrey JE and Newcomb WW (1984) Laser-induced fluorescence of green plants 1: A technique for the remote detection of plant stress and species differentiation. Appl Opt 23: 134-138
10. Chappelle EW, McMurtrey JE and Kim MS (1991) Identification of the pigment responsible for the blue fluorescence band in the laser induced fluorescence (LIF) spectra of green plants, and the potential use of the band in remotely estimating rates of photosynthesis Remote Sens Environ 36: 213-218
11. Duysens LNM and Amesz J (1957) Fluorescence spectrophotometry of reduced phosphopyridine nucleotide in intact cells in the near-ultraviolet and visible region. Biochim Biophys Acta 24: 19-26
12. Duysens LNM and Kronenberg GHM (1957) The fluorescence spectrum of the complex of reduced phosphopyridine nucleotide and alcohol dehydrogenase from yeast. Biochim Biophys Acta 26: 437-438
13. Duysens LNM and Sweep G (1957) Fluorescence spectrophotometry of pyridine nucleotide in photosinthesizing cells. Biochim Biophys Acta 25: 13-16
14. Goulas Y, Moya I and Schmuck G (1990) Time-resolved spectroscopy of the blue fluorescence of spinach leaves Photosynth Res 25: 299-307
15. Harbig K, Chance B, Kovach AGB and Reivich M (1976) In vivo measurement of pyridine nucleotide fluorescence from cat brain cortex. J Appl Physiol 41: 480-488
16. Heineman FW and Balaban RS (1993) Effects of afterload and heart rate on NAD(P)H redox state in the isolated rabbit heart. Am J Physiol 264: H433-H440
17. Kobayashi S, Nishiki K, Kaede K and Ogata E (1971) Optical consequences of blood substitution on tissue oxidation-reduction state microfluorimetry. J Appl Physiol 31: 93-96
18. Kramer DM and Crofts AR (1996) Control and measurement of photosynthetic electron transport in vivo. In: Baker NR (ed) Photosynthesis and the Environment, pp 25-66. Kluwer Academic Publishers, Dordrecht, The Netherlands
19. Kwong SCW and Rao G (1994) Metabolic monitoring by using the rate of changes of NAD(P)H fluorescence. Biotechnol Bioeng 44: 453-459
20. Lang M, Stober F and Lichtenthaler HK (1991) Fluorescence emission spectra of plant leaves and plant constituents Radiat Environ Biophys 30: 333-347
21. Lang M, Siffel P, Braunova Z and Lichtenthaler HK (1992) Investigations of the blue-green fluorescence emission of plant leaves. Bot Acta 105: 435-440
22. Laughlin MR and Heineman FW (1994) The relationship between phosphorylation potential and redox state in the isolated working rabbit heart. J Mol Cell Cardiol 26: 1525-1536
23. Miller J (1981) Standardsin Fluorescence Spectrometry. Techniques in Visible and Ultraviolet Spectrometry, pp 1-49-67. Chapman and Hall, London
24. Morales F, Cerovic ZG and Moyal (1994) Characterization of blue-green fluorescence in the mesophyll of sugar beet (Beta vulgaris L.) leaves affected by iron deficiency. Plant Physiol 106: 127-133
25. Morales F, Cerovic ZG and Moya I (1996) Time-resolved blue-green fluorescence of sugar beet (Beta vulgaris L.) leaves Spectroscopic evidence for the presence of ferulic acid as the main fluorophore in the epidermis Biochim Biophys Acta 1273: 251-262
26. Olson JM and Amesz J (1960) Action spectra for fluorescence excitation of pyridine nucleotide in photosynthetic bacteria and algae. Biochim Biophys Acta 37: 14-24
27. Olson JM, Duysens LNM and Kronenberg GHM (1959) Spectrofluorometry of pyridine nucleotide reactions in Chromatium. Biochim Biophys Acta 36: 125-131
28. Rao G and Mutharasan R (1989) NADH levas and solventogenesisin Clostridium acetobutylicum: new insights trough culture fluorescence. Appl Microbiol Biotechnol 30: 59-66
29. Reardon KF, Scheper T-H and Bailey TE (1987) Metabolic pathway rates and culture fluorescence in batch fermentations of Clostridium Acetobutylicum. Biotech Prog 3: 153-167
30. Salmon J-M, Kohen E, Viallet P, Hirschberg JG, Wouters AW, Kohen C and Thorell B (1982) Microspectrofluorimetric approach to the study of free/bound NAD(P)H ratio as metabolic indicator in various cell types. Photochem Photobiol 36: 585-593
31. Schreiber U, Neubauer C and Schliwa U (1993) PAM fluorometer based on medium-frequency pulsed Xe-flash measuring light: A highly sensitive new tool in basic and applied photosynthesis research. Photosynth Res 36: 65-72
32. Scott DA, Grotyohann LW, Cheung JY and Scaduto RC (1994) Ratiometric methodology for NAD(P)H measurement in the perfused rat heart using surface fluorescence. Am J Physiol 267: H636-H644
33. Scott TG, Spencer RD, Leonard NL and Weber G (1970) Emission properties of NADH. Studies of florescence lifetimes and quantum efficiencies of NADH, AcPyADH, and simplified synthetic models J Am Chem Soc 92: 687-695
34. Srinivas SP and Mutharasan R (1987) Inner filter effects and their interferences in the interpretation of culture fluorescence. Biotechnol Bioeng 30: 769-774
35. Stober F and Lichtenthaler HK (1992) Changes of the laser-induced blue, green and red fluorescence signatures during greening of etiolated leaves of wheat. J Plant Physiol 140: 673-680
36. Stober F and Lichtenthaler HK (1993a) Studies on the constancy of the blue and green fluorescence yield during the chlorophyll fluorescence induction kinetics (Kautsky effect). Radiat Environ Biophys 32: 357-365
37. Stober F and Lichtenthaler HK (1993b) Studies on the localization and spectral characteristics of the fluorescence emission of differently pigmented wheat leaves Bot Acta 106: 365-370
38. Stober F, Lang M and Lichtenthaler HK (1994) Blue, green, and red fluorescence emission signatures of green, etiolated, and white leaves Remote Sens Environ 47: 65-71
39. Velick SF (1961) In: McElroy WD and Glass B (eds) Light and Life, pp 108-143. Johns Hopkins Press, Baltimore, MD
40. Wakita M, Nishimura G and Tamura M (1995) Some characteristics of the fluorescence lifetime of reduced pyridine nucleotides in isolated mitochondria, isolated hepatocytes, and perfused rat liver in situ. J Biochem Tokyo 118: 1151-1160
41. Walker D, Cerovic Z and Robinson S (1987) Isolation of intact chloroplasts General principles and criteria of integrity. Methods Enzymol 148: 145-157
42. Wang NS and Simmons MB (1991) Characterization of an on-line commercial fluorescence probe: Modeling of the probe signal. Biotechnol Bioeng 38: 1292-1301
43. Weber G (1958) Transfert d'énergie dans la dihydro-diphosphopyridine nudéotide. J Chem Phys 55: 878-886
44. Wolfbeis OS (1985) The fluorescence of organic natural products In: Schulman SG (ed) Molecular Luminescence Spectroscopy. Methods and Applications: Part 1, pp 167-317. John Wley & Sons, New York
45. Wu J, Feld MS and Rava RP (1993) Analytical model for extracting intrinsic fluorescence in turbid media. Appl Opt 32: 3585-3595