Increasing the transfection efficacy and subsequent long-term culture of resting human pancreatic duct epithelial cells

Pancreas

Volumn 17, Issue 3, 1998, Pages 262-265

  Jesnowski, Ralf ^a   Liebe, Stefan ^a   Löhr, J Matthias ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Immortalization; Pancreas; Pancreatic duct epithelial cells; SV40 large T antigen; Transfection

Indexed keywords

BUFFER; CALCIUM PHOSPHATE; SODIUM CHLORIDE; VIRUS LARGE T ANTIGEN; VIRUS SMALL T ANTIGEN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL IMMORTALIZATION; CONTROLLED STUDY; EPITHELIUM CELL; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; NONHUMAN; PANCREAS DUCT; PH; PRECIPITATION; PRIORITY JOURNAL;

EID: 0031695218     PISSN: 08853177     EISSN: None     Source Type: Journal    
DOI: 10.1097/00006676-199810000-00006     Document Type: Article

References (15)

1. Ausubel FM, Brent R, Kingston RE, Seidman JG, Smith JA, Struhl K. Current protocols in molecular biology. Brooklyn, NY: Greene Publisher, 1994.
2. Ray J, Gage FH. Gene transfer into established and primary fibroblast cell lines: comparison of transfection methods and promoters. Biotechniques 1992;13:598-603.
3. Keown WA, Campbell CR. Kucherlapati RS. Methods for introducing DNA into mammalian cells. In: Goeddel DV, ed. Gene expression technology. San Diego, CA: Academic Press, 1990: 527-37.
4. Löhr M, Jesnowski R, Zauner I, et al. SV40-immortalized bovine pancreatic duct cells retain morphological and functional characteristics of their native counterpart (submitted).
5. Price J, Turner D, Cepko C. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc Natl Acad Sci USA 1987;84:156-60.
6. Trautmann B, Schlitt HJ, Hahn EG, Löhr M. Isolation, culture, and characterization of human pancreatic duct cells. Pancreas 1993;8: 248-54.
7. Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet 1982;1:327-41.
8. Löhr M, Trautmann B, Göttler M, et al. Human ductal adenocarcinomas of the pancreas express extracellular matrix proteins. Br J Cancer 1994;69:144-51.
9. Löhr M, Trautmann B, Göttler M, et al. Expression and function of receptors for extracellular matrix proteins in human ductal adenocarcinomas of the pancreas. Pancreas 1996;12:248-59.
10. Bett AJ, Haddara W, Prevec L, Graham FL. An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proc Natl Acad Sci USA 1994;91:8802-6.
11. Davis HL, Whalen RG, Dememeix BA. Direct gene transfer into skeletal muscle in vivo: factors affecting efficiency of transfer and stability of expression. Hum Gene Ther 1993;4:151-9.
12. Schwartz B, Benoist C, Abdallah B, et al. Gene transfer by naked DNA into adult mouse brain. Gene Ther 1996;3:405-11.
13. Jiang CK, Conolly D, Blumenberg M. Comparison of methods for transfection of human epidermal keratinocytes. J Invest Dermatol 1991;97:969-73.
14. Becker TC, Noel RJ, Coats WS, et al. Use of recombinant adenovirus for metabolic engineering of mammalian cells. Methods Cell Biol 1994;43:161-89.
15. Günzburg WH, Salmons B. Development of retroviral vectors as safe, targeted gene delivery systems. J Mol Med 1996;74:171-82.