Defects in base excision repair combined with elevated intracellular dCTP levels dramatically reduce mutation induction in yeast by ethyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine

Environmental and Molecular Mutagenesis

Volumn 32, Issue 2, 1998, Pages 173-178

  Kunz, Bernard A ^a   Henson, Elizabeth S ^b   Karthikeyan, Ramachandran ^a   Kuschak, Theodore ^b   McQueen, Shelley A ^b   Scott, Catherine A ^b   Xiao, Wei ^c  

^a DEAKIN UNIVERSITY   (Australia)

^b UNIVERSITY OF MANITOBA   (Canada)

^c UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Alkylation mutagenesis; Apurinic sites; DNA precursor pools; EMS; MNNG; Yeast

Indexed keywords

DEOXYCYTIDINE PHOSPHATE; MESYLIC ACID ETHYL ESTER; METHYLNITRONITROSOGUANIDINE;

ALKYLATION; AMINO ACID SUBSTITUTION; ARTICLE; DNA REPLICATION; DNA SYNTHESIS; ENZYME ACTIVITY; EUKARYOTE; EXCISION REPAIR; GENE FREQUENCY; GENE INDUCTION; GENE MUTATION; MUTAGENESIS; PROKARYOTE; SACCHAROMYCES CEREVISIAE; YEAST;

ALKYLATING AGENTS; ALKYLATION; CARBON-OXYGEN LYASES; DEOXYCYTOSINE NUCLEOTIDES; DEOXYRIBONUCLEASE IV (PHAGE T4-INDUCED); DNA ADDUCTS; DNA DAMAGE; DNA GLYCOSYLASES; DNA LIGASES; DNA REPAIR; DNA, FUNGAL; DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE; ETHYL METHANESULFONATE; FUNGAL PROTEINS; GENES, SUPPRESSOR; INTRACELLULAR FLUID; METHYLNITRONITROSOGUANIDINE; MUTAGENESIS; N-GLYCOSYL HYDROLASES; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; PROKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0031694471     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2280(1998)32:2<173::AID-EM13>3.0.CO;2-M     Document Type: Article

References (27)

1. Alani EL, Cao L, Kleckner N (1987): A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116:541-564.
2. Beranek DT (1990): Distribution of methyl and ethyl adducts following alkylation with monofunctional alkylating agents. Mutat Res 231:11-30.
3. Berdal KG, Bjørås M, Bjelland S, Seeberg E (1990): Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisaie; a homologue to the bacterial alkA gene. EMBO J 9:4563-4568.
4. Bishop DK, Andersen J, Kolodner RD (1989): Specificity of mismatch repair following transformation of Saccharomyces cerevisiae with heteroduplex plasmid DNA. Proc Natl Acad Sci USA 86:3713-3717.
5. Chen J, Derfler B, Maskati A, Samson L (1989): Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in Escherichia coli. Proc Natl Acad Sci USA 86:7961-7965.
6. Dianov G, Lindahl T (1994): Reconstitution of the DNA base excisionrepair pathway. Curr Biol 4:1069-1076.
7. Dianov G, Price A, Lindahl T (1992): Generation of single-nucleotide repair patches following excision of uracil residues from DNA. Mol Cell Biol 12:1605-1612.
8. Dipple A (1995): DNA adducts of chemical carcinogens. Carcinogenesis 16:437-441.
9. Frosina G, Fortini P, Rossi O, Carrozzino F, Raspaglio G, Cox LS, Lane DP, Abbondandolo A, Dogliotti E (1996): Two pathways for base excision repair in mammalian cells. J Biol Chem 271:9573-9578.
10. Gietz D, St Jean A, Woods RA, Schiestl RH (1992): Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 20:1425.
11. Kohalmi SE, Kunz BA (1988): Roles of neighbouring bases and assessment of strand specificity in ethylmethanesulphonate and Nmethyl-N′-nitro-N-nitrosoguanidine in the SUP4-o gene of Saccharomyces cerevisiae. J Mol Biol 204:561-568.
12. Kohalmi L, Kunz BA (1992): In vitro mutagenesis of the yeast SUP4-o gene to identify all substitutions that can be detected in vivo with the SUP4-o. Environ Mol Mutagen 19:282-287.
13. Kohami SE, Glattke M, McIntosh EM, Kunz BA (1991): Mutational specificity of DNA precursor pool imbalances in yeast arising from deoxycytidylate deaminase deficiency or treatment with thymidylate. J Mol Biol 220:933-946.
14. Kohalmi SE, Roche HM, Kunz BA (1993): Elevated intracellular dCTP levels reduce the induction of GC → AT transitions in yeast by ethyl methanesulfonate or N-methyl-N'-′nitro-N-nitrosoguanidine but increase alkylation-induced GC → CG transversions. Mutagenesis 8:457-465.
15. Kramer B, Kramer W, Williamson MS, Fogel S (1989): Heteroduplex DNA correction in Saccharomyces cerevisiae is mismatch specific and requires functional PMS genes. Mol Cell Biol 9:4432-4440.
16. Kunz BA, Pierce MK, Mis JRA, Giroux CN (1987): DNA sequence analysis of the mutational specificity of u.v, light in the SUP4-o gene of yeast, Mutagenesis 2:445-453.
17. Kunz BA, Gabriel M, Kang X, Kohalmi SE, Terrick K (1992): DNA repair modifies the site and strand specificity of ethyl methanesulfonate mutagenesis in yeast. Mutagenesis 7:461-469.
18. Kunz BA, Henson ES, Roche H, Ramotar D, Nunoshiba T, Demple B (1994a): Specificity of the mutator caused by deletion of the yeast structural gene (APNI) for the major apurinic endonuclease. Proc Natl Acad Sci USA 91:8165-8169.
19. Kunz BA, Kohalmi SE, Kunkel TA, Mathews CK, McIntosh EM, Reidy JA (1994b): Deoxyribonucleoside triphosphate levels: A critical factor in the maintenance of genetic stability. Mutat Res 318:1-64.
20. Loeb LA, Preston BD (1986): Mutagenesis by apurinic/apyrimidinic sites. Annu Rev Genet 20:210-230.
21. Mis JRA, Kunz BA (1990): Analysis of mutations induced in the SUP4-o gene of Saccharomyces cerevisiae by cis-diammine dichloroplatinum(II). Carcinogenesis 11:633-638.
22. Newlon CS (1988): Yeast chromosome replication and segregation. Microbiol Rev 52:568-601.
23. Pierce MK, Giroux CN, Kunz BA (1987): Development of a yeast system to assay mutational specificity. Mutat Res 182:65-74.
24. Ramotar D, Popoff SC, Gralla EB, Demple B (1991): Cellular role of yeast Apnl apurinic endonuclease/3′diesterase: Repair of oxidative and alkylation damage and control of spontaneous mutation. Mol Cell Biol 11:4537-4544.
25. Roche H, Gietz RD, Kunz BA (1995): Specificities of the Saccharomyces cerevisiae rad6, rad18 and rad52 mutators exhibit different degrees of dependence on the REV3 gene product, a putative nonessential DNA polymerase. Genetics 140:443-456.
26. Wang SS, Hopper AK (1988): Isolation of a yeast gene involved in species-specific tRNA processing. Mol Cell Biol 8:5140-5149.
27. Xiao W, Samson L (1993): In vivo evidence for endogenous DNA alkylation damage as a source of spontaneous mutation in eukaryotic cells. Proc Natl Acad Sci USA 90:117-2121.