Large DNA fragment sizing using native acrylamide gels on an automated DNA sequencer and GENESCAN(TM) software

BioTechniques

Volumn 25, Issue 3, 1998, Pages 464-470

  McEvoy, Christopher R E ^a   Seshadri, Ram ^a,b   Firgaira, Frank A ^a,b  

^a FLINDERS UNIVERSITY   (Australia)

^b FLINDERS MEDICAL CENTRE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; COMPUTER PROGRAM; DNA SEQUENCE; GENE AMPLIFICATION; MODEL; NUCLEOTIDE SEQUENCE; ONCOGENE H RAS;

ALLELES; DNA; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT DYES; GENES, RAS; HUMANS; MICROSATELLITE REPEATS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, DNA; SOFTWARE;

EID: 0031691982     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Ardern, J.C., M.J. Saunders, K. Hyde, R. Lawson, J.A. Liu Yin and G.S. Lucas. 1993. Polymerase chain reaction analysis of allele frequency and loss at the Harvey Ras locus in myeloid malignancies. Leukemia 7:258-262.
2. Capon, D.J., E.Y. Chen, A.D. Levinion, P.H. Seeburgy and D.V. Goeddel. 1983. Complete nucleotide sequence of the T24 human bladder carcinoma oncogene and its normal homologue, Nature 302:33-37.
3. Frégeau, C.J. and R.M. Fourney. 1993, DNA typing with fluorescently tagged short tandem repeats: a sensitive and accurate approach to human identification, BioTechniques 15:100-119.
4. Garrett, P.A., B.S. Hulka, Y.L. Kim and R.A. Farber. 1993. HRAS proto oncogene polymorphism and breast cancer. Cancer Epidemiol, Biomarkers Prev. 2:131-138.
5. Krontiris, T.G., B. Devlin, D.D. Carp, N.J. Robert and N. Risch. 1993, An association between the risk of cancer and mutations in the HRAS1 minisatellite locus. N. Engl. J. Med. 329:517-523.
6. Krontiris, T.G., N.A. DiMarino, M. Colb and D.R. Parkinson. 1985, Unique allelic restriction fragments of the human Ha-ras locus in leukocyte and tumour DNA's of cancer patients, Nature 313:369-374.
7. Maniatis, T., E.F. Fritsch and J. Sambrook, 1982. Molecular Cloning: A Laboratory Manual. CSH Laboratory, Cold Spring Harbor, NY.
8. Mansfield, D.C., A.F. Brown, D.K. Green, A.D. Carothers, S.W. Morris, H.J. Evans and A.F. Wright. 1994. Automation of genetic linkage analysis using fluorescent microsatellite markers. Genomics 24:225-233.
9. Patterson, H.D. and R. Thompson. 1971. Recovery of inter-block information when block sizes are unequal, Biometrika 58:545-554.
10. Phelan, C.M., T.R. Rebbeck, B.L. Weber, P. Deville, M.H. Ruttledge, H.T. Lynch, G.M. Lenoir, M.R. Stratton et al. 1996. Ovarian cancer risk in BRCA1 carriers is modified by the HRAS1 variable number of tandem repeat (VNTR) locus. Nature Genetics 12:309-311.
11. Reed, P.W., J.L. Davies, J.B. Copeman, S.T. Bennett, S.M. Palmer, L.E. Pritchard, S.C.L. Gough, Y. Kauraguchi et al. 1994. Chromosome-specific microsatellite sets for fluoresecence-based, semi-automated genome mapping. Nature Genetics 7:390-395.
12. Song, S. 1995, Application of fluorescent dUTP on PCR and automated PCR product detection by using internal lane size standard. Nucleic Acids Symp, Ser. 34:211-213.
13. Wolff, R.K., Y. Nakamura and R. White. 1988. Molecular characterization of a spontaneously generated new allele at a VNTR locus: no exchange of flanking DNA sequence. Genomics 3:347-351.
14. Ziegle, J.S., Y. Su, K.P. Corcoran, L. Nie, P.E. Maynard, L.B. Hoff, L.J. McBride, M.N. Kronick and S.R. Dlehl. 1992. Application of automated DNA sizing technology for genotyping microsatellite loci, Genomics 14:1026-1031.