Detection of urinary tract infections by reduction of nitroblue tetrazolium: Technical note

Kidney International

Volumn 54, Issue 4, 1998, Pages 1331-1336

  Smith, Shannon D ^a   Wheeler, Marcia A ^a   Weiss, Robert M ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

NADH; NADPH; Nitric oxide synthase; Superoxide dismutase; UTI

Indexed keywords

NITRIC OXIDE SYNTHASE;

ADULT; AGED; ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; ENZYME ACTIVITY; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; NITROBLUE TETRAZOLIUM TEST; PRIORITY JOURNAL; SCREENING TEST; URINALYSIS; URINARY TRACT INFECTION; URINE CULTURE;

EID: 0031688447     PISSN: 00852538     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1755.1998.00102.x     Document Type: Article

References (42)

1. SMITH SD, WHEELER MA, WEISS RM: Nitric oxide synthase: An endogenous source of elevated nitrite in infected urine. Kidney Int 45:586-591, 1994
2. WHEELER MA, SMITH SD, GARCÍA-CARDEÑA G, NATHAN CF, WEISS RM, SESSA WC: Bacterial infection induces nitric oxide synthase in human neutrophils. J Clin Invest 99:110-116, 1997
3. HOPE BT, MICHAEL GJ, KNIGGE KM, VINCENT SR: Neuronal NADPH diaphorase is a nitric oxide synthase. Proc Natl Acad Sci USA 88:2811-2814, 1991
4. LIEW FY, COX FE: Nonspecific defense mechanism: The role of nitric oxide. Immunol Today 12:A17-A21, 1991
5. FLESCH IEA, HESS JH, KAUFMANN SH: NADPH diaphorase staining suggests a transient and localized contribution of nitric oxide to host defense against an intracellular pathogen in situ. Int Immunol 6:1751-1757, 1994
6. CALKA J, WOLF G, SCHMIDT W: Induction of cytosolic NADPH-diaphorase/nitric oxide synthase in reactive microglia/macrophages after quinolinic acid lesions in the rat striatum: An electron and light microscopical study. Histochem Cell Biol 105:81-89, 1996
7. NICHOLSON S, BONECINI-ALMEIDA M DA G, LAPA E SILVA JR, NATHAN C, XIE QW, MUMFORD R, WEIDNER JR, CALAYCAY J, GENG J, BOECHAT N: Inducible nitric oxide synthase in pulmonary alveolar macrophages from patients with tuberculosis. J Exp Med 183:2293-2302, 1996
8. PIVA E, DE TONI S, CAENAZZO A, PRADELLA M, PIETROGRANDE F, PLEBANI M: Neutrophil NADPH oxidase activity in chronic myeloproliferative and myelodysplastic disease by microscopic and photometric assays. Acta Haematol 94:16-22, 1995
9. NETER E: Evaluation of the tetrazolium test for the diagnosis of significant bacteriuria. JAMA 192:769-770, 1965
10. BULGER RJ, KIRBY WMM: Simple tests for significant bacteriuria. Arch Intern Med 112:742-746, 1963
11. STANSFELD JM: The measurement and meaning of pyuria. Arch Dis Child 37:257-262, 1962
12. BRANSON D: Evaluation of a rapid nitrite screening test for bacteriuria. Am J Clin Pathol 46:698-701, 1966
13. JAMES GP, PAUL KL, FULLER JB: Urinary nitrite and urinary tract infection. Am J Clin Pathol 70:671-678, 1978
14. LACHS MS, NACHAMKIN I, EDELSTEIN PH, GOLDMAN J, FEINSTEIN, AR, SCHWARZ JS: Spectrum bias in the evaluation of diagnostic tests: Lessons from the rapid dipstick test for urinary tract infection. Ann Intern Med 117:135-140, 1992
15. SMALLEY DL: Effect of ascorbic acid on leukocyte esterase and hemoglobin test strips. (abstract) Clin Chem 31:1243, 1985
16. CZERWINSKI AW, WILKERSON RG, MERRILL JA, BRADEN BB, COLMORE JP: Further evaluation of the Griess test to detect significant bacteriuria. II. Am J Obstet Gynecol 110:677-681, 1971
17. POU S, POU WS, BREDT DS, SNYDER SH, ROSEN GM: Generation of superoxide by purified brain nitric oxide synthase. J Biol Chem 267:24173-24176, 1992
18. ROMERO M, MOSQUERA J, RODRIGUEZ-ITURBE B: A simple method to identify NBT-positive cells in isolated glomeruli. Nephrol Dial Transplant 12:174-179, 1997
19. ASSREUY J, CUNHA FQ, EPPERLEIN M, NORONHA-DUTRA A, O'DONNELL CA, LIEW FY, MONCADA S: Production of nitric oxide and superoxide by activated macrophages and killing of Leishmania major. Eur J Immunol 24:672-676, 1994
20. WEVER RMF, VAN DAM T, VAN RIJN HJ, DE GROOT F, RABELINK TJ: Tetrahydrobiopterin regulates superoxide and nitric oxide generation by recombinant endothelial nitric oxide synthase. Biochem Biophys Res Commun 237:340-341, 1997
21. STUEHR DJ, FASEHUN OA, KWON NS, GROSS SS, GONZALEZ JA, LEVI R, NATHAN CF: Inhibition of macrophage and endothelial cell nitric oxide synthase by diphenyleneiodonium and its analogs. FASEB J 5:98-103, 1991
22. CROSS AR, JONES OTG: The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labeling of a component polypeptide of the oxidase. Biochem J 237:111-116, 1986
23. GROZDANOVIC Z, GOSSRAV R: Nonspecific alkaline phosphatase activity can be responsible for staining of NADPH-diaphorase activity in certain non-neural cells. Folia Histochem Cytobiol 33:3-10, 1995
24. LI Y, SEVERN A, ROGERS MV, PALMER RM, MONCADA S, LIEW FY: Catalase inhibits nitric oxide synthesis and the killing of intracellular Leishmania major in murine macrophages. Eur J Immunol 22:441-446, 1992
25. NAGI MN, AL-BEKAIRI AM, AL-SAWAF HA: Spectrophotometric assay for superoxide dismutase based on the nitroblue tetrazolium reduction by glucose-glucose oxidase. Biochem Mol Biol Int 36:633-638, 1995
26. THOROGOOD MC, ARMSTEAD WM: Influence of polyethylene glycol superoxide dismutase/catalase on altered opioid-induced pial artery dilation after brain injury. Anesthesiology 84:614-625, 1996
27. FAIR WR: Urinary tract infections-Current status. Semin Urol 1:91-96, 1983
28. STAMM WE, COUNTS GW, RUNNING, KR, FIHN S, TURCK M, HOLMES KK: Diagnosis of coliform infection in acutely dysuric women. N Engl J Med 307:463-468, 1982
29. LIOCHEV SI, BATINIC-HABERLE I, FRIDOVICH I: The effect of detergents on the reduction of tetrazolium salts. Arch Biochem Biophys 324:48-52, 1995
30. MITCHELL JA, KOHLHAAS KL, MATSUMOTO T, POLLOCK JS, FORSTERMANN U, WARNER TD, SCHMIDT HH, MURAD F: Induction of NADPH-dependent diaphorase and nitric oxide synthase activity in aortic smooth muscle and cultured macrophages. Mol Pharmacol 41:1163-1168, 1992
31. BROWN BW JR, HOLLANDER M: Statistics, A Biomedical Introduction. New York, John Wiley & Sons, Inc, 1977, pp 201-224
32. YANAGISAWA N: Adhesive properties of bacteria isolated from patients with urinary tract infection to the urinary bladder. Nippon Hinyokika Gakkai Zasshi 88:24-34, 1997
33. GALLEY HF, WALKER BE, HOWDLF PD, WEBSTER NR: Regulation of nitric oxide synthase activity in cultured human endothelial cells: Effect of antioxidants. Free Radic Biol Med 21:97-101, 1996
34. BAFHNER RL, NATHAN DG: Quantitative nitroblue tetrazolium test in chronic granulomatous disease. N Engl J Med 278:971-976, 1968
35. KRETSCHMER RR, PAPIERNIAK CK, STEWARDSON-KRIEGER P, BAMZAI AK, GOTOFF SP: Quantitative nitrobluctetrazolium reduction by normal newborn monocytes. J Pediatr 91:306-309, 1977
36. FOWLER JE JR: Office bacteriology: Techniques and interpretations. Semin Urol 1:97-105, 1983
37. LACE JK, TAN JS, WATANAKUNAKORN C: An appraisal of the nitroblue tetrazolium reduction test. Am J Med 58:685-694, 1975
38. SEGAL AW: Nitroblue-tetrazolium tests. Lancet 2:1248-1252, 1974
39. SMITH SD, WHEELER MA, ZHANG R, WEISS ED, LORBER MI, SESSA WC, WEISS RM: Nitric oxide synthase induction with renal transplant rejection or infection. Kidney Int 50:2088-2093, 1996
40. GORDON AM, BRIGGS JD, BELL PRF: The nitroblue tetrazolium (NBT) test in renal transplantation. J Clin Pathol 27:734-737, 1974
41. WOLF G: Nitric oxide and nitric oxide synthase: Biology, pathology, localization. Histol Histopathol 12:251-261, 1997
42. EWING JF, JANERO DR: Microplate superoxide dismutase assay employing a nonenzymatic superoxide generator. Anal Biochem 232: 243-248, 1995