Low-density induced apoptosis of cortical neurons is inhibited by serum factors

Cellular and Molecular Neurobiology

Volumn 18, Issue 5, 1998, Pages 487-496

  Sasaki, Yukio ^a   Fukushima, Nobuyuki ^a,b   Yoshida, Akira ^b   Ueda, Hiroshi ^b  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

^b NAGASAKI UNIVERSITY   (Japan)

Author keywords

Alamar Blue; Apoptosis; Cell density dependency; Neuronal cell death; Primary culture

Indexed keywords

DNA FRAGMENT; INDICATOR;

ANIMAL CELL; APOPTOSIS; ARTICLE; BRAIN CELL; CELL DENSITY; CELL SURVIVAL; CONTROLLED STUDY; CULTURE MEDIUM; FETUS; NERVE CELL CULTURE; NERVE CELL NECROSIS; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; RAT; SERUM FACTOR; SIGNAL TRANSDUCTION;

ANIMALS; APOPTOSIS; BIOLOGICAL FACTORS; CATTLE; CELLS, CULTURED; CEREBRAL CORTEX; COLORING AGENTS; CULTURE MEDIA, SERUM-FREE; DNA FRAGMENTATION; FETAL BLOOD; NEURONS; OXAZINES; OXIDATION-REDUCTION; RATS; RATS, WISTAR; XANTHENES;

EID: 0031682631     PISSN: 02724340     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026375225275     Document Type: Article

References (16)

1. 3H]thymidine incorporation assay. J. lmmunol. Methods 170:211-224.
2. Chun, L. Y., and Patterson, P. H. (1977). Role of nerve growth factor in the development of rat sympathetic neurons in vitro. I. Survival, growth, and differentiation of catecholamine production. J. Cell Biol. 75:694-704.
3. Davies, A. M. (1994). Intrinsic programmes of growth and survival in developing vertebrate neurons. Trends Neurosci. 17:195-199.
4. Edwards, S. N., Buckmaster, A. E., and Tolkovsky, A. M. (1991). The death programme in cultured sympathetic neurons can be suppressed at the posttranslational level by nerve growth factor, cyclic AMP, and depolarization. J. Neurochem. 57:2140-2143.
5. Fukushima, N., Ueda, H., and Misu, Y. (1994). Identification of novel endogenous factors against neuronal cell death in serum-free primary cultures. Soc. Neurosci. Abstr. p. 686.
6. Ishizaki, Y., Burne, J. F., and Raff, M. C. (1994). Autocrine signals enable chondrocytes to survive in culture. J. Cell Biol. 126:1069-1077.
7. Kiernam, J. A. (1973). In Kiernam, J. A. (ed.), Oxidoreductase, Pergamon, Oxford.
8. Labarca, C., and Paigen, K. (1980). A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102:344-352.
9. Mosmann, T. (1983). Rapid calorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. lmmunol. Methods 65:55-63.
10. Raff, M. C. (1992). Social controls on cell survival and cell death. Nature 356:397-400.
11. Raff, M. C., Barres, B. A., Burne, J. F., Coles, H. S., Ishizaki, Y., and Jacobson, M. D. (1993). Programmed cell death and the control of cell survival: Lessons from the nervous system. Science 262:695-700.
12. Re, F., Zanetti, A., Sironi, M., Polentarutti, N., Lanfrancone, L., Dejana, E., and Colotta, F. (1994). Inhibition of anchorage-dependent cell spreading triggers apoptosis in cultured human endothelial cells. J. Cell Biol. 127:537-546.
13. Roehm, N. W., Rodgers, G. H., Hatfield, S. M., and Glasebrook, A. L. (1991). An improved calorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J. lmmunol. Methods 142:257-265.
14. Scott, B. S., and Fisher, K. C. (1970). Potassium concentration and number of neurons in cultures of dissociated ganglia. Exp. Neural. 27:16-22.
15. Scudiero, D. A., Shoemaker, R. H., Paull, K. D., Monks, A., Tierney, S., Nofziger, T. H., Currens, M. J., Seniff, D., and Boyd, M. R. (1988). Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 48:4827-4833.
16. Shiba, Y., Yamada, S., and Kanno, Y. (1989). Survival of 3T3-L1 cells induced by fibroblast growth factor depends on cell density and adhesion to the substratum. Cell Struct. Funct. 14:473-483.