Construction of a single-copy integration vector and its use to study gene expression in Bacillus licheniformis

Microbiology

Volumn 144, Issue 9, 1998, Pages 2573-2578

  Tran, Lam Son Phan ^a   Szabó, Lóránd ^a   Orosz, László ^a   Sík, Tibor ^a   Holczinger, András ^a  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

Author keywords

Bacitracin synthetase; Campbell type recombination; Gene expression; lacZ fusions; NTG mutagenesis

Indexed keywords

BACITRACIN; BETA GALACTOSIDASE;

ARTICLE; BACILLUS LICHENIFORMIS; BACTERIAL GENE; ESCHERICHIA COLI; GENE CONTROL; GENE EXPRESSION; GENETIC RECOMBINATION; MICROCOCCUS; NONHUMAN; OPERON; PRIORITY JOURNAL;

BACILLUS LICHENIFORMIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MICROCOCCUS; MICROCOCCUS LUTEUS; POSIBACTERIA;

EID: 0031669914     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-144-9-2573     Document Type: Article

References (28)

1. Axel, M., Hayward, R. & Schumann, W. (1996). Integrative vectors for constructing single-copy transcriptionsl fusions between Bacillus subtilis promoters and various reporter genes encoding heat-stable enzymes. Gene 182, 33-36.
2. Bolivar, F., Rodriguez, R. L, Greene, P. J., Betlach, M. C., Heynecker, H. L., Boyer, H. W., Crosa, J. H. & Falkow, S. (1977). Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2, 95.
3. Bron, S. (1990). Plasmids. In Molecular Biological Methods for Bacillus, pp. 75-174. Edited by C. R. Harwood & S. M. Cutting. Chichester: Wiley.
4. Cutting, S. M. & Vander Horn, P. B. (1990). Genetic analysis. In Molecular Biological Methods for Bacillus, pp. 27-74. Edited by C. R. Harwood & S. M. Cutting. Chichester: Wiley.
5. Errington, J. (1990). Gene cloning techniques. In Molecular Biological Methods for Bacillus, pp. 175-220. Edited by C. R. Harwood & S. M. Cutting. Chichester: Wiley.
6. Farinha, M. A. & Kropinski, A. M. (1990). Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters. J Bacteriol 172, 3496-3499.
7. Harwood, C.R. (1992). Bacillus subtilis and its relatives: molecular biological and industrial workhorses. Trends Biotechnol 10, 247-256.
8. Holczinger, A. (1986). Improvement of bacitracin production and bacteriophages of Bacillus licheniformis. PhD thesis, Budapest Technical University.
9. Kalnins, A., Otto, K., Ruther, U. & Muller-Hill, B. (1983). Sequence of the lacZ gene of Escherichia coli. EMBO J 2, 593-597.
10. Kleinkauf, H. & von Döhren, H. (1987). Biosynthesis of peptide antibiotics. Annu Rev Microbiol 41, 259-289.
11. Lewandoski, M. & Smith, I. (1988). Use of a versatile lacZ vector to analyze the upstream region of the Bacillus subtilis spoOF gene. Plasmid 20, 148-154.
12. McCuen, R. W. & Thorne, C. B. (1971). Genetic mapping of genes concerned with glutamyl polypeptide production by Bacillus licheniformis and a study of their relationship to the development of competence for transformation. J Bacterial 107, 636-645.
13. 70 consensus promoters in Psendomonas aeruginosa and Escherichia coli. Can J Microbiol 43, 981-985.
14. Meima, R., Venema, G. & Bron, S. (1996). A positive selection vector for the analysis of structural plasmid instability in Bacillus subtilis. Plasmid 35, 14-30.
15. Miller, J. H. (1972). Experiments in Molecular Genetics, pp. 352-355. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
16. Nakamura, K. & lnouye, M. (1982). Construction of versatile expression cloning vehicles using the lipoprotetn gene of Escherichia coli. EMBO J 1, 771-775.
17. Olsen, R. H., DeBusscher, G. & McCrombie, W. R. (1982). Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol 150, 60-69.
18. Perego, M. (1993). Integrational vectors for genetic manipulation in Bacillus subtilis. In Bacillus subtilis and Other Gram-Positive Bacteria, pp. 615-624. Edited by A. L. Sonenshein, J. A. Hoch & R. Losick. Washington, DC: American Society for Microbiology.
19. Prágai, Z. (1994). Transposon mutagenesis and protoplast transformation of Bacillus licheniformis. PhD thesis, Hungarian Academy of Sciences.
20. Prágai, Z., Holczinger, A. & Sík, T. (1994). Transformation of Bacillus licheniformis protoplasts by plasmid DNA. Microbiology 140, 305-310.
21. Prágai, Z., Tjalsma, H., Bolhuis, A., van Dijl, J. M., Venema, G. & Bron, S. (1997). The signal peptidase II (lsp) gene of Bacillus subtilis. Microbiology 143, 1327-1333.
22. Ruther, U., Koenen, M., Otto, K. & Muller-Hill, B. (1981). pUR222, a vector for cloning and rapid chemical sequencing of DNA. Nucleic Acids Res 9, 4087-4098.
23. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
24. Shimotsu, H. & Henner, D. J. (1986). Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 43, 85-94.
25. Sík, T., Horváth, J. & Chatterjee, S. (1980). Generalized transduction in Rhizobium meliloti. Mol Gen Genet 178, 511-516.
26. Slauch, J. M. & Silhavy, T. J. (1991). Genetic fusions as experímental tools. Methods Enzymol 204, 213-248.
27. Tangney, M., Jørgensen, P. L., Diderichsen, B. & Jørgensen, S. T. (1995). A new method for integration and stable DNA amplification in poorly transformable bacilli. FEMS Microbiol Lett 125, 107-144.
28. Thorne, C. B. & Kowalski, J. B. (1976). Temperate bacteriophages for Bacillus licheniformis. In Microbiology, pp. 303-314. Edited by D. Schlessinger. Washington, DC: American Society for Microbiology.