PCR bias Toward the wild-type k-ras and p53 sequences: Implications for PCR detection of mutations and cancer diagnosis

BioTechniques

Volumn 25, Issue 4, 1998, Pages 684-691

  Barnard, Ross ^a   Futo, V ^a   Pecheniuk, N ^a   Slattery, M ^a   Walsh, T ^a  

^a QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P53;

AMINO ACID SEQUENCE; ARTICLE; CANCER DIAGNOSIS; DNA SEQUENCE; GENE TARGETING; OLIGONUCLEOTIDE PROBE; ONCOGENE K RAS; POINT MUTATION; POLYMERASE CHAIN REACTION;

EID: 0031667780     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98254dt08     Document Type: Article

References (20)

1. Bertina, R.M., B.P.C. Koeleman, T. Koster, F.R. Rosendaal, R.J. Dirven, H. de Ronde, P.A. van der Velden and P.H. Reitsma. 1994. Mutation in blood coagulation factor V associated with resistance to activated protein C. Nature 369:64-67.
2. Hasegawa, Y., S. Takeda, S. Ichii, K. Koizumi, M. Maruyama, A. Fujii, H. Ohta, T. Nakajima, M. Okuda, S. Baba and Y. Nakamura. 1995. Detection of k-ras mutations in DNAs isolated from feces of patients with colorectal tumors by mutant-allele-specific-amplification (MASA). Oncogene 10:1441-1445.
3. He, Q., M. Marjamaki, H. Soini, J. Mertsola and M.J. Viljanen. 1994. Primers are decisive for sensitivity of PCR. BioTechniques 17:82-87.
4. Hirao, I., Y. Nishimura, T. Naraoka, K. Watanabe, Y. Arata and K. Miura. 1989. Extraordinary stable structure of short single stranded DNA fragments containing a specific base sequence: d(GCGAAAGC). Nucleic Acids Res. 17:2223-2231.
5. Jazwinska, E.C., L.M. Cullen, F. Busfield, W.R. Pyper, S.I. Webb, L.W. Powell, C.P. Morris and T.P. Walsh. 1996. Haemochromatosis and HLA-H. Nature Genet. 14:249-251.
6. Khan, I.M. and J.M. Coulson. 1993. A novel method to stabilize antisense oligonucleotides against exonuclease degradation. Nucleic Acids Res. 21:2957-2958.
7. Liu, Q., E.C. Thorland and S.S. Sommer. 1997. Inhibition of PCR amplification by a point mutation downstream of a primer. BioTechniques 22:292-300.
8. Pecheniuk, N.M., N.A. Marsh, T.P. Walsh and J.L. Dale. 1997. Use of first nucleotide change technology to determine the frequency of factor V Leiden in a population of Australian blood donors. Blood Coagul. Fibrinolysis. 8:491-495.
9. Poort, S.R., J.S. Michiels, P.H. Reitsma and R.M. Bertina. 1994. Homozygosity for a novel missense mutation in the prothrombin gene causing a severe bleeding disorder. Thromb. Haemost. 72:819-824.
10. Raeymakers, L. 1993. Quantitative PCR: theoretical considerations with practical implications. Anal. Biochom. 214:582-585.
11. Saiki, R.K. 1989. Optimization of the polymerase chain reaction, p. 26. In H.A. Erlich, R. Gibbs and H.H. Kazazian (Eds.), Current Communications in Molecular Biology Polymerase Chain Reaction. CSH Laboratory Press, Cold Spring Harbor, NY.
12. Samadashwily, G.M., A. Dayn and S.M. Mirkin. 1993. Suicidal nucleotide sequences for DNA polymerization. EMBO J. 12:4975-4983.
13. Sidransky, D., T. Tokino, S.R. Hamilton, K.W. Kinzler, B. Levin, P. Frost and B. Vogelstein. 1992. Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors. Science 256:102-105.
14. Smith-Ravin, J., J. England, I.C. Talbot and W. Bodmer. 1995. Detection of c-Ki-ras mutations in faecal samples from sporadic colorectal cancer patients. Gut 36:81-86.
15. Stolovitsky, G. and G. Cecchi. 1996. Efficiency of DNA replication in the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 93:12947-12952.
16. Stork, P., M. Loda, S. Bosari, B. Wiley, K. Poppenhusen and H. Wolfe. 1991. Detection of K-ras mutations in pancreatic and hepatic neoplasms by non-isotopic mismatched polymerase chain reaction. Oncogene 6:857-862.
17. Van Dongen, M.J.P., M.M.W. Mooren, E.F.A. Willens, G.A. van der Marel, J.H. van Boom, S.S. Wijmenga and C.W. Hilbers. 1997. Structural features of the DNA hairpin d(ATCCTA-GTTA-TAGGAT):formation of a G-A base pair in the loop. Nucleic Acids Res. 25:1537-1547.
18. Vogelstein, B., E.R. Fearon, S.R. Hamilton, S.E. Kern, A.C. Prefsinger, M. Leppert, Y. Nakamura, R. White, A.M.M. Smits and J.L. Bos. 1988. Genetic alterations during colorectal-tumor development. N. Engl. J. Med. 319:525-532.
19. Walsh, P.S., H.A. Erlich and R. Higuchi. 1992. Preferential PCR amplification of alleles: mechanisms and solutions. PCR Methods Appl. 1:241-250.
20. Ward, R.L., F. Santiago, N.J. Hawkins, D. Coomber, T. O'Connor and A.V. Todd. 1995. A rapid PCR ELISA for the detection of activated K-ras in colorectal cancer. J. Clin. Pathol. 48:273-277.