The hybrid formation between Aspergillus oryzae var. oryzae and Penicillium chrysogenum by nuclear transfer and the production of alkaline protease

Korean Journal of Applied Microbiology and Biotechnology

Volumn 26, Issue 4, 1998, Pages 290-296

  Yang, Young Ki ^a   Kang, Hee Kyoung ^a   Lim, Chae Young ^a   Moon, Myeng Nim ^a  

^a Chosun University   (South Korea)

Author keywords

Alkaline protease; Aspergillus oryzae var. oryzae; Nuclear transfer; Penicillium chrysogenum

Indexed keywords

ALKALINE PROTEINASE;

ARTICLE; ASPERGILLUS ORYZAE; BACTERIUM MUTANT; DNA CONTENT; GENETIC STABILITY; HYBRID; NONHUMAN; PENICILLIUM CHRYSOGENUM; PROTEIN DEGRADATION; PROTOPLAST;

ASPERGILLUS ORYZAE; PENICILLIUM CHRYSOGENUM;

EID: 0031666639     PISSN: 02572389     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Bradshaw, R. E., K. U. Lee, and J. F. Peberdy. 1983. Aspects of genetic interaction in hybrids of Aspergillus nidulans and A. rugulosus by protoplast fusion. J. Gen. Microbiol. 129: 3525-3533.
2. Chung, K. C., K. Kawai, S. Yoshima, and Y. Eguchi. 1982. Production of cellulolytic enzymes by Penicillium verruculosum. Hakkokogaku 60: 355-357.
3. Ferenczy, L., F. Kevei, M. Franco, and I. Rojik. 1976. Factors affecting high frequency fungal protoplast fusion. Experimentia 32: 1156-1158.
4. Ferenczy, L. 1983. Current question of gene transfer via protoplast fusion in microorganism. 6th Inter. Protoplast Symposium pp. 137-142.
5. Ferenczy, L. and M. Pesti. 1982. Transfer of nuclei into protoplasts of Saccharomyces cerevisiae. Curr. Microbiol. 7: 157-160.
6. Giles, K. W. and W. Myers. 1965. An improved diphenylamine method for estimation of deoxyribonucleic acid. Nature 206: 93.
7. Hartly, B. S. 1960. Ann. Rev. Biochem. 29: 459.
8. Hong, S. W., Y. C. Hah, and H. M. Park. 1984. The conidial protoplast fusion of cellulolytic fungus, Trichoderma koningii. Kor. J. Microbiol. 22: 207-213.
9. Horikoshi, K. and T. Akiba. 1971. Production of alkaline enzymes by alkalophilic microorganisms. Part I. Alkaline protease produced by Bacillus sp. No. 221. Agric. Biol. Chem. 35: 1401-1414.
10. Jonsson, U. and B. G. Snygg. 1974. Lipase production and activity as a function of incubation time, pH and temperature of four lipolytic microorganisms. J. Appl. Bacteriol. 37: 571-581.
11. Kao, K. N. and M. R. Michayuk. 1974. A method for high frequency intergeneric fusion of plant protoplasts. Planta 115: 355-367.
12. Laemmli, U. K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
13. Lee, J. A., J. S. Maeng, P. J. Maeng, and Y. H. Rhee. 1989. Synergistic effect of substrate on the biosynthesis of cellulase and xylanase complexes from Aspergillus nidulans. Kor. J. Mycol. 17: 57-65.
14. Lorz, H. and I. Potrykus. 1978. Investigation on the transfer of isolated nuclei into protoplasts. Theor. Appl. Genet. 53: 251-256.
15. Margesin, R. and F. Schinner. 1992. Production and properties of an extracellular metalloprotease from a psychrophilic Pseudomonas fluorescens. J. Biotech. 24: 207-210.
16. Min, K. R. 1987. Isolation and analysis of intra- and interspecific hybrids in genus Trichoderma. M. S. thesis, Seoul National University.
17. Nakamura, M. 1959. J. Agri. Chem. Sci. Japan 33: 586.
18. Nakanishi, T., Y. Matsmura, N. Minamiura, and T. Yamamoto. 1974. Purification and some properties of an alkalophilic proteinase of an a Streptomyces sp. Agric. Biol. Chem. 38: 37-44.
19. Nevalainen, K. M. H. and E. T. Palva. 1978. Production of extracellular enzymes in mutants isolated from Trichoderma viride. J. Biochem. 71: 999-1007.
20. Peberdy, J. F., C. E. Buckley, D. C. Daltrey, and P. M. Moore. 1976. Factors affecting protoplast release in some filamentous fungi. Trans. Br. Mycol. Soc. 67(1): 23-26.
21. Peberdy, J. F. 1980. Protoplast Fusion: A New Approach Interspecific Genetic Manipulation and Breeding in Fungi, pp. 63-71. Pergamon Press.
22. Rho, H. S. 1991. Study on interspecific hybrid formation by nuclear transfer in Aspergillus spp. Department Biology, Graduate School, Chungnam Nat'l. Univ.
23. Svoboda, A. 1981. Polyethylene glycol induced membrane fusion in yeast protoplasts. Acta Histochemica 23: 211-217.
24. Toyama, H., K. Yamaguchi, A. Shinmyo, and H. Okada. 1984. Protoplast fusion of Trichoderma reesei using immature conidia. Appl. Environ. Microbiol. 47: 363-368.
25. Yang, Y. K., Y. Park, Y. H. Rhee, and P. J. Maeng. 1989. Study on formation of Aspergillus nidulans hybrids by protoplast fusion and nuclear transfer. Kor. J. Mycol. 17: 154-160.