Detection of TLS/FUS-CHOP fusion transcripts in myxoid and round cell liposarcomas by nested reverse transcription-polymerase chain reaction using archival paraffin-embedded tissues

Diagnostic Molecular Pathology

Volumn 7, Issue 2, 1998, Pages 96-101

  Hisaoka, Masanori ^a   Tsuji, Seiji ^a   Morimitsu, Yosuke ^a   Hashimoto, Hiroshi ^a   Shimajiri, Shohei ^a   Komiya, Setsuro ^b   Ushijima, Masahiro ^c  

^a UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH   (Japan)

^b KURUME UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c Kitakyushu Municipal Hospital   (Japan)

Author keywords

Fusion gene; Myxoid liposarcoma; Nested reverse transcription polymerase chain reaction; Paraffin embedded tissue; Translocation

Indexed keywords

BETA ACTIN; MESSENGER RNA; PARAFFIN;

ADOLESCENT; ADULT; AGED; ARTICLE; CHROMOSOME ANALYSIS; CONTROLLED STUDY; FEMALE; GENE; GENETIC TRANSCRIPTION; HUMAN; HUMAN TISSUE; LIPOSARCOMA; MALE; MYXOSARCOMA; PRIORITY JOURNAL; RECIPROCAL CHROMOSOME TRANSLOCATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADOLESCENT; ADULT; CCAAT-ENHANCER-BINDING PROTEINS; CHROMOSOMES, HUMAN, PAIR 12; CHROMOSOMES, HUMAN, PAIR 16; FEMALE; HUMANS; LIPOSARCOMA; LIPOSARCOMA, MYXOID; MALE; MIDDLE AGED; NEOPLASM PROTEINS; NUCLEAR PROTEINS; ONCOGENE PROTEINS, FUSION; PARAFFIN EMBEDDING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, NEOPLASM; RNA-BINDING PROTEIN FUS; SOFT TISSUE NEOPLASMS; TRANSCRIPTION FACTOR CHOP; TRANSLOCATION, GENETIC; TUMOR MARKERS, BIOLOGICAL;

EID: 0031665689     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/00019606-199804000-00006     Document Type: Article

References (29)

1. Adams V, Hany MA, Schmid M, Hassam S, Briner J, Niggli FK. Detection of t(11;22)(q24;q12) translocation breakpoint in paraffin-embedded tissue of the Ewing's sarcoma family by nested reverse transcription-polymerase chain reaction. Diagn Mol Pathol 1996;5:107-13
2. Aman P, Ron D, Mandahl N, et al. Rearrangement of the transcription factor gene CHOP in myxoid liposarcoma with t(12;16)(q13;p11). Genes Chromosom Cancer 1992;5:278-85.
3. Aoki T, Hisaoka M, Kouho H, Hashimoto H, Nakata H. Interphase cytogenetic analysis of myxoid soft tissue tumors by fluorescence in situ hybridization and DNA flow cytometry using paraffin-embedded tissue. Cancer 1997;79:284-93.
4. Dal Cin P, Sciot R, Panagopoulos I, et al. Additional evidence of a variant translocation t(12;22) with EWS/CHOP fusion in myxoid liposarcoma: clinicopathological features. J Pathol 1997;182:437-41.
5. Delattre O, Zucman J, Plougastel B, et al. Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours. Nature 1992;359:162-5.
6. Dorsey BV, Benjamin LE, Rauscher FR, Klencke B, Benook AP, Warren RS, Weidner N. Intra-abdominal desmoplastic small round-cell tumor: expansion of the pathologic profile. Mod Pathol 1996;9:703-9.
7. Edwards RH, Chatten J, Xiong Q-B, Barr FG. Detection of gene fusions in rhabdomyosarcoma by reverse transcriptase-polymerase chain reaction assay of archival samples. Diagn Mol Pathol 1997; 6:91-7.
8. Enzinger FM, Weiss SW. Liposarcoma. In: Enzinger FM, Weiss SW, eds. Soft tissue tumors, 3rd ed. St Louis: Mosby, 1995:431-66.
9. Finke J, Fritzen R, Ternes P, Lange W, Dölken G. An improved strategy and a useful housekeeping gene for RNA analysis from formalin-fixed, paraffin-embedded tissues by PCR. Biotechniques 1993;14:448-53.
10. Fletcher CDM, Akerman M, DalCin P, et al. Correlation between clinicopathological features and karyotype in lipomatous tumors. A report of 178 cases from the chromosomes and morphology (CHAMP) collaborative study group. Am J Pathol 1996;148:623-30.
11. Fligman I, Lonardo F, Jhanwar SC, Gerald WL, Woodruff J, Ladanyi M. Molecular diagnosis of synovial sarcoma and characterization of a variant SYT-SSX2 fusion transcript. Am J Pathol 1995; 147:1592-9.
12. Foss RD, Guha-Thakurta N, Conran RM, Gutman P. Effects of fixative and fixation time on the extraction and polymerase chain reaction amplification of RNA from paraffin-embedded tissue. Comparison of two housekeeping gene mRNA controls. Diagn Mol Pathol 1994;3:148-55.
13. Hisaoka M, Hashimoto H, Murao T. Peripheral primitive neuroectodermal tumour with ganglioneuroma-like areas arising in the cauda equina. Virchows Arch 1997;431:365-9.
14. Knight JC, Renwick PJ, Dal Cin P, Van den Berghe H, Fletcher CDM. Translocation t(12;16)(q13;p11) in myxoid liposarcoma and round cell liposarcoma: molecular and cytogenetic analysis. Cancer Res 1995;55:24-7.
15. Kuroda M, Ishida T, Horiuchi H, et al. Chimeric TLS/FUS-CHOP gene expression and the heterogeneity of its junction in human myxoid and round cell liposarcoma. Am J Pathol 1995;147: 1221-7.
16. Kuroda M, Ishida T, Takahashi M, et al. Oncogenic transformation and inhibition of adipocytic conversion of preadipocytes by TLS/ FUS-CHOP type II chimeric protein. Am J Pathol 1997;151:735-44.
17. Labelle Y, Zucman J, Stenman G, et al. Oncogenic conversion of a novel orphan nuclear receptor by chromosome translocation. Hum Mol Genet 1995;4:2219-26.
18. Ladanyi M, Gerald W. Fusion of the EWS and WT1 genes in the desmoplastic small round cell tumor. Cancer Res 1994;54:2837-40.
19. Mazzella F, Johnson C, Tallini G, Crotty PL. Lack of expression of SYT-SSX1 mRNA in spindle cell sarcoma other than synovial sarcoma. Mod Pathol 1996;9:10A.
20. Panagopoulos I, Hoglund M, Mertens F, Mandahl N, Mitelman F, Aman P. Fusion of the EWS and CHOP genes in myxoid liposarcoma. Oncogene 1996;12:489-94.
21. Panagopoulos I, Mandahl N, Ron D, et al. Characterization of the CHOP breakpoints and fusion transcripts in myxoid liposarcomas with the 12;16 translocation. Cancer Res 1994;54:6500-3.
22. Panagopoulos I, Lassen C, Isaksson M, Mitelman F, Mandahl N, Aman P. Characteristic sequence motif at the breakpoints of the hybrid genes FUS/CHOP, EWS/ERG in myxoid liposarcoma and acute myeloid leukemia. Oncogene 1997;15:1357-62.
23. Ron D, Habener JF. CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant negative inhibitor of gene transcription. Genes Dev 1992;6:439-53.
24. Sanchez GI, Rabbitts TH. Transcription activation by TAL1 and FUS-CHOP proteins expressed in acute malignancies as a result of chromosomal abnormalities. Proc Natl Acad Sci USA 1994;91: 7869-73.
25. Scotlandi K, Serra M, Manara MC, et al. Immunostaining of the p30/32MIC2 antigen and molecular detection of EWS rearrangements for the diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumor. Hum Pathol 1996;27:408-16
26. Tallini G, Akerman M, Dal Cin P, et al. Combined morphologic and karyotypic study of 28 myxoid liposarcomas. Implications for a revised morphologic typing. A report from the CHAMP group. Am J Surg Pathol 1996;20:1047-55.
27. Zinszner H, Albalat R, Ron D. A novel effector domain from the RNA-binding protein TLS or EWS is required for oncogenic transformation by CHOP. Genes Dev 1994;8:2513-26.
28. Zucman J, Delattre O, Desmaze C, et al. EWS and ATF-1 gene fusion induced by t(12;22) translocation in malignant melanoma of soft parts. Nature Genet 1993;4:341-5.
29. Zucman J, Melot T, Desmaze C, et al. Combinatorial generation of variable fusion proteins in the Ewing family of tumours. EMBO J 1993;12:4481-7.