New trimethoprim-resistant dihydrofolate reductase cassette, dfrXV, inserted in a class 1 integron

Antimicrobial Agents and Chemotherapy

Volumn 42, Issue 9, 1998, Pages 2221-2224

  Adrian, Peter V ^a,c   Du Plessis, Mignon ^a   Klugman, Keith P ^a   Amyes, Sebastian G B ^b  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c NATIONAL HEALTH LABORATORY SERVICE   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROFOLATE REDUCTASE; SPECTINOMYCIN; SULFONAMIDE; TRIMETHOPRIM;

AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; ESCHERICHIA COLI; INTEGRON; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

EID: 0031664163     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/aac.42.9.2221     Document Type: Article

References (32)

1. Adrian, P. V., C. J. Thomson, K. P. Klugman, and S. G. B. Amyes. 1995. A novel dihydrofolate reductase cassette inserted in an integron borne on a Tn21-like element abstr. C87, p. 55. In Program and abstracts of the 35th Interscience Conference on Antimicrobial Agents and Chemotherapy. American Society for Microbiology, Washington, D.C.
2. Adrian, P. V., C. J. Thomson, K. P. Klugman, and S. G. B. Amyes. 1995. Prevalence of trimethoprim resistant dihydrofolate reductase genes identified with oligonucleotide probes in plasmids from South Africa isolates of commensal faecal flora. J. Antimicrob. Chemother. 35:497-508.
3. Adrian, P. V., C. J. Thomson, K. P. Klugman, and S. G. B. Amyes. 1995. Prevalence and genetic location of non-tranferable trimethoprim resistant dihydrofolate reductase genes in South Africa commensal faecal flora. Epidemiol. Infect. 115:255-267.
4. Amyes, S. G. B., and I. M. Gould. 1984. Trimethoprim resistance plasmids in faecal bacteria. Ann. Microbiol. (Inst. Pasteur). 135B.177-186.
5. Amyes, S. G. B., and J. T. Smith. 1974. R-factor trimethoprim resistance mechanism: an insusceptible target site. Biochem. Biophys. Res. Commun. 58:412-418.
6. Amyes, S. G. B., and J. T. Smith. 1976. The purification properties of the trimethoprim-resistant dihydrofolate reductase mediated by the R-factor, R388. Eur. J. Biochem. 61:597-603.
7. Amyes, S. G. B., K. J. Towner, G. I. Carter, C. J. Thomson, and H.-K. Young. 1989. The type VII dihydrofolate reductase: a novel plasmid-encoded trimethoprim-resistant enzyme from gram-negative bacteria isolated in Britain. J. Antimicrob. Chemother. 24:111-119.
8. Burchall, J. J., and G. H. Hitchings. 1965. Inhibitor binding analysis of dihydrofolate reductases from various species. Mol. Pharmacol. 1:126-136.
9. Collis, C. M., G. Grammaticopoulos, J. Briton, H. W. Stokes, and R. M. Hall. 1993. Site-specific insertion of gene cassettes into integrons. Mol. Microbiol. 9:41-52.
10. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16: 6127-6145.
11. Fling, M. E., and C. Richards. 1983. Nucleotide sequence of the trimethoprim resistant dihydrofolate reductase gene harboured by Tn7. Nucleic Acids Res. 11:5147-5158.
12. Hall, R. M., and C. Vockler. 1987. The region of the IncN plasmid R46 coding for resistance to β-lactam antibiotics, streptomycin/spectinomycin and sulphonamides is closely related to antibiotic resistance segments found in IncW plasmids and in Tn21-like transposons. Nucleic Acids Res. 15:7491-7501.
13. Hollingshead, S., and D. Vapnek. 1985. Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenyltransferase. Plasmid 13: 17-30.
14. Huovinen, P., L. Sundström, G. Swedburg, and O. Sköld. 1995. Trimethoprim and sulfonamide resistance. Antimicrob. Agents Chemother. 39:279-289.
15. Lévesque, C., S. Brassard, J. Lapointe, and P. H. Roy. 1994. Diversity and relative strength of tandem promoters for the antibiotic-resistance genes of several integrons. Gene 142:49-54.
16. Martinez, E., and F. de la Cruz. 1988. Transposon Tn21 encodes a RecA-independent site-specific integration system. Mol. Gen. Genet. 211:320-325.
17. Novak, P., D. Stone, and J. J. Burchall. 1983. R-plasmid dihydrofolate reductase with a dimeric subunit structure. J. Biol. Chem. 258:10956-10959.
18. Osborn, M. J., and F. M. Huennekens. 1958. Enzymatic reduction of dihydrofolic acid. J. Biol. Chem. 233:969-974.
19. Ouellette, M., and P. H. Roy. 1987. Homology of ORFs from Tn2603 and from R46 to site-specific recombinases. Nucleic Acids Res. 15:10055.
20. Pattishall, K. H., J. Acar, J. Burchall, F. W. Goldstein, and R. J. Harvey. 1977. Two distinct types of trimethoprim-resistant dihydrofolate reductase specified by R-plasmids of different compatibility groups. J. Biol. Chem. 252: 2319-2323.
21. Rådstrom, P., O. Sköld, G. Swedberg, J. Flensburg, P. H. Roy, and L. Sundström. 1994. Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements. J. Bacteriol. 176:3257-3268.
22. Recchia, G. D., and R. M. Hall. 1995. Gene cassettes: a new class of mobile element. Microbiology 141:3015-3027.
23. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Sundström, L., P. Rådström, G. Swedberg, and O. Sköld. 1988. Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sull and a recombination active locus of Tn21. Mol. Gen. Genet. 213:191-201.
25. Sundström, L., and O. Sköld. 1990. The dhfrI trimethoprim resistance gene of Tn7 can be found at specific sites in other genetic surroundings. Antimicrob. Agents Chemother. 34:642-650.
26. Sundström, L., G. Swedberg. and O. Sköld. 1993. Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance. J. Bacteriol. 175:1796-1805.
27. Thomson, C. J., and S. G. B. Amyes. 1988. Biochemical properties of the type V plasmid-encoded trimethoprim resistant dihydrofolate reductase. J. Pharm. Pharmacol. 40:21.
28. Wylie, B. A., S. G. B. Amyes, H.-K. Young, and H. J. Koornhof. 1988. Identification of a novel plasmid-encoded dihydrofolate reductase mediating high-level resistance to trimethoprim. J. Antimicrob. Chemother. 22:429-435.
29. Wylie, B. A., and H. J. Koornhof. 1991. Nucleotide sequence of dihydrofolate reductase type VI. J. Med. Microbiol. 35:214-218.
30. Young, H.-K., and S. G. B. Amyes. 1985. Characterisation of a new transposon-mediated trimethoprim-resistant dihydrofolate reductase. Biochem. Pharmacol. 34:4334-4337.
31. Young, H.-K., M. J. Qumsieh, and M. L. McIntosh. 1994. Nucleotide sequence and genetic analysis of the type Ib trimethoprim-resistant, Tn4132-encoded dihydrofolate reductase. J. Antimicrob. Chemother. 34:715-725.
32. Zühlsdorf, M. T., and B. Wiedemann. 1993. Functional and physiological characterisation of the Tn21 cassette for resistance genes in Tn2426. J. Gen. Microbiol. 139:995-1002.