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Volumn 64, Issue 10, 1998, Pages 4021-4027

Purification and substrate specificities of two α-L- arabinofuranosidases from Aspergillus awamori IFO 4033

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA ARABINOFURANOSIDASE; ARABINAN; ARABINOSE; ARABINOXYLAN; OLIGOSACCHARIDE; POLYSACCHARIDE; UNCLASSIFIED DRUG;

EID: 0031662792     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.10.4021-4027.1998     Document Type: Article
Times cited : (58)

References (49)
  • 1
    • 84940981726 scopus 로고
    • Structure and function of plant cell walls
    • J. Preiss (ed.), Academic Press, San Diego, Calif.
    • Bacic, A., P. J. Harris, and B. A. Stone. 1988. Structure and function of plant cell walls, p. 297-371. In J. Preiss (ed.), The biochemistry of plants, vol. 14. Academic Press, San Diego, Calif.
    • (1988) The Biochemistry of Plants , vol.14 , pp. 297-371
    • Bacic, A.1    Harris, P.J.2    Stone, B.A.3
  • 4
    • 0029903563 scopus 로고    scopus 로고
    • Cloning and expression of the alpha-L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae
    • Crous, J. M., I. S. Pretorius, and W. H. van Zyl. 1996. Cloning and expression of the alpha-L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 46:256-260.
    • (1996) Appl. Microbiol. Biotechnol. , vol.46 , pp. 256-260
    • Crous, J.M.1    Pretorius, I.S.2    Van Zyl, W.H.3
  • 6
    • 0027772796 scopus 로고
    • Cloning and characterization of the abfB gene coding for the major α-L-arabinofuranosidase (ABF B) of Aspergillus niger
    • Flipphi, M. J. A., M. van Heuvel, P. van der Veen, J. Visser, and L. H. de Graaff. 1993. Cloning and characterization of the abfB gene coding for the major α-L-arabinofuranosidase (ABF B) of Aspergillus niger. Curr. Genet. 24: 525-532.
    • (1993) Curr. Genet. , vol.24 , pp. 525-532
    • Flipphi, M.J.A.1    Van Heuvel, M.2    Van Der Veen, P.3    Visser, J.4    De Graaff, L.H.5
  • 8
    • 0028151030 scopus 로고
    • Arabinase gene expression in Aspergillus niger: Indications for coordinated regulation
    • Flipphi, M. J. A., J. Visser, P. van der Veen, and L. H. de Graaff. 1994. Arabinase gene expression in Aspergillus niger: indications for coordinated regulation. Microbiology 140:2673-2682.
    • (1994) Microbiology , vol.140 , pp. 2673-2682
    • Flipphi, M.J.A.1    Visser, J.2    Van Der Veen, P.3    De Graaff, L.H.4
  • 9
    • 77955764774 scopus 로고
    • A rapid permethylation of glycolipid and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide
    • Hakomori, S. 1964. A rapid permethylation of glycolipid and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J. Biochem. 55: 205-208.
    • (1964) J. Biochem. , vol.55 , pp. 205-208
    • Hakomori, S.1
  • 10
    • 0001496526 scopus 로고
    • α-L-Arabinofuranosidase from radish (Raphanus sativus L.) seeds
    • Hata, K., M. Tanaka, Y. Tsumuraya, and Y. Hashimoto. 1992. α-L-Arabinofuranosidase from radish (Raphanus sativus L.) seeds. Plant Physiol. 100: 388-396.
    • (1992) Plant Physiol. , vol.100 , pp. 388-396
    • Hata, K.1    Tanaka, M.2    Tsumuraya, Y.3    Hashimoto, Y.4
  • 11
    • 0029929874 scopus 로고    scopus 로고
    • Updating the sequence-based classification of glycosyl hydrolases
    • Henrissat, B., and A. Bairoch. 1996. Updating the sequence-based classification of glycosyl hydrolases. Biochem. J. 316:695-696.
    • (1996) Biochem. J. , vol.316 , pp. 695-696
    • Henrissat, B.1    Bairoch, A.2
  • 13
    • 0014959631 scopus 로고
    • Purification, crystallization and amino acid composition of α-L-arabinofuranosidase from Aspergillus niger
    • Kaji, A., and K. Tagawa. 1970. Purification, crystallization and amino acid composition of α-L-arabinofuranosidase from Aspergillus niger. Biochim. Biophys. Acta 207:456-464.
    • (1970) Biochim. Biophys. Acta , vol.207 , pp. 456-464
    • Kaji, A.1    Tagawa, K.2
  • 14
    • 0014666480 scopus 로고
    • Properties of purified α-L-arabinofuranosidase from Aspergillus niger
    • Kaji, A., K. Tagawa, and T. Ichimi. 1969. Properties of purified α-L-arabinofuranosidase from Aspergillus niger. Biochim. Biophys. Acta 171:186-188.
    • (1969) Biochim. Biophys. Acta , vol.171 , pp. 186-188
    • Kaji, A.1    Tagawa, K.2    Ichimi, T.3
  • 15
    • 0038760938 scopus 로고
    • Studies on the enzymes acting on araban. VIII. Purification and properties of arabanase produced by Aspergillus niger
    • Kaji, A., K. Tagawa, and K. Matsubara. 1967. Studies on the enzymes acting on araban. VIII. Purification and properties of arabanase produced by Aspergillus niger. Agric. Biol. Chem. 31:1023-1028.
    • (1967) Agric. Biol. Chem. , vol.31 , pp. 1023-1028
    • Kaji, A.1    Tagawa, K.2    Matsubara, K.3
  • 16
    • 0029397813 scopus 로고
    • Substrate specificity of α-L-arabinofuranosidase from Bacillus subtilis 3-6 toward arabinofuranooligosaccharides
    • Kaneko, S., and I. Kusakabe. 1995. Substrate specificity of α-L-arabinofuranosidase from Bacillus subtilis 3-6 toward arabinofuranooligosaccharides. Biosci. Biotechnol. Biochem. 59:2132-2133.
    • (1995) Biosci. Biotechnol. Biochem. , vol.59 , pp. 2132-2133
    • Kaneko, S.1    Kusakabe, I.2
  • 17
    • 0027636874 scopus 로고
    • Purification and some properties of intracellular α-L-arabinofuranosidase from Aspergillus niger 5-16
    • Kaneko, S., T. Shimasaki, and I. Kusakabe. 1993. Purification and some properties of intracellular α-L-arabinofuranosidase from Aspergillus niger 5-16. Biosci. Biotechnol. Biochem. 57:1161-1165.
    • (1993) Biosci. Biotechnol. Biochem. , vol.57 , pp. 1161-1165
    • Kaneko, S.1    Shimasaki, T.2    Kusakabe, I.3
  • 18
    • 0028168108 scopus 로고
    • Purification and some properties of α-L-arabinofuranosidase from Bacillus subtilis 3-6
    • Kaneko, S., M. Sano, and I. Kusakabe. 1994. Purification and some properties of α-L-arabinofuranosidase from Bacillus subtilis 3-6. Appl. Environ. Microbiol. 60:3425-3428.
    • (1994) Appl. Environ. Microbiol. , vol.60 , pp. 3425-3428
    • Kaneko, S.1    Sano, M.2    Kusakabe, I.3
  • 19
    • 0028968328 scopus 로고
    • The core trisaccharide of α-L-arabinofuranan: Synthesis of methyl 3,5-di-O-α-L-arabinofuranosyl-α-L-arabinofuranoside
    • Kaneko, S., Y. Kawabata, T. Ishii, Y. Gama, and I. Kusakabe. 1995. The core trisaccharide of α-L-arabinofuranan: synthesis of methyl 3,5-di-O-α-L-arabinofuranosyl-α-L-arabinofuranoside. Carhohydr. Res. 268:307-311.
    • (1995) Carhohydr. Res. , vol.268 , pp. 307-311
    • Kaneko, S.1    Kawabata, Y.2    Ishii, T.3    Gama, Y.4    Kusakabe, I.5
  • 20
    • 0028893958 scopus 로고
    • Synthesis of regioisomeric methyl α-L-arabinofuranobiosides
    • Kawabata, Y., S. Kaneko, I. Kusakabe, and Y. Gama. 1995. Synthesis of regioisomeric methyl α-L-arabinofuranobiosides. Carbohydr. Res. 267:39-47.
    • (1995) Carbohydr. Res. , vol.267 , pp. 39-47
    • Kawabata, Y.1    Kaneko, S.2    Kusakabe, I.3    Gama, Y.4
  • 21
    • 0001349462 scopus 로고
    • Preparation of some aryl α-L-arabinofuranosides as substrates for arabinofuranosidase
    • Kelly, M. A., M. L. Sinnott, and D. Widdows. 1988. Preparation of some aryl α-L-arabinofuranosides as substrates for arabinofuranosidase. Carbohydr. Res. 181:262-266.
    • (1988) Carbohydr. Res. , vol.181 , pp. 262-266
    • Kelly, M.A.1    Sinnott, M.L.2    Widdows, D.3
  • 22
    • 0027907989 scopus 로고
    • Mode of action of (1→4)-β-D-arabinoxylan arabinofuranohydrolase (AXH) and α-L-arabinofuranosidases on alkali-extractable wheat-flour arabinoxylan
    • Kormelink, F. J. M., H. Gruppen, and A. G. J. Voragen. 1993. Mode of action of (1→4)-β-D-arabinoxylan arabinofuranohydrolase (AXH) and α-L-arabinofuranosidases on alkali-extractable wheat-flour arabinoxylan. Carbohydr. Res. 249:345-353.
    • (1993) Carbohydr. Res. , vol.249 , pp. 345-353
    • Kormelink, F.J.M.1    Gruppen, H.2    Voragen, A.G.J.3
  • 23
    • 0029947087 scopus 로고    scopus 로고
    • Release of feruric acid from sugar-beet pulp by using arabinanase, arabinofuranosidase and an esterase from Aspergillus niger
    • Kroon, P. A., and G. Williamson. 1996. Release of feruric acid from sugar-beet pulp by using arabinanase, arabinofuranosidase and an esterase from Aspergillus niger. Biotechnol. Appl. Biochem. 23:263-267.
    • (1996) Biotechnol. Appl. Biochem. , vol.23 , pp. 263-267
    • Kroon, P.A.1    Williamson, G.2
  • 24
    • 0000089019 scopus 로고
    • Structures of the arabinoxylo-oligosaccharides from the hydrolytic products of corn cob arabinoxylan by a xylanase from Streptomyces
    • Kusakabe, I., S. Ohgushi, T. Yasui, and T. Kobayashi. 1983. Structures of the arabinoxylo-oligosaccharides from the hydrolytic products of corn cob arabinoxylan by a xylanase from Streptomyces. Agric. Biol. Chem. 47:2713-2723.
    • (1983) Agric. Biol. Chem. , vol.47 , pp. 2713-2723
    • Kusakabe, I.1    Ohgushi, S.2    Yasui, T.3    Kobayashi, T.4
  • 25
    • 85007706673 scopus 로고
    • Some properties of araban degrading enzymes produced by microorganisms and enzymatic preparation of arabinose from sugar beet pulp
    • Kusakabe, I., T. Yasui, and T. Kobayashi. 1975. Some properties of araban degrading enzymes produced by microorganisms and enzymatic preparation of arabinose from sugar beet pulp. Nippon Nogeikagaku Kaishi 49:295-305.
    • (1975) Nippon Nogeikagaku Kaishi , vol.49 , pp. 295-305
    • Kusakabe, I.1    Yasui, T.2    Kobayashi, T.3
  • 26
    • 85007662210 scopus 로고
    • Preparation of crystalline xylooligosaccharides from xylan
    • Kusakabe, I., T. Yasui, and T. Kobayashi. 1975. Preparation of crystalline xylooligosaccharides from xylan. Nippon Nogeikagaku Kaishi 49:383-385.
    • (1975) Nippon Nogeikagaku Kaishi , vol.49 , pp. 383-385
    • Kusakabe, I.1    Yasui, T.2    Kobayashi, T.3
  • 27
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of baeteriophage T4
    • Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of baeteriophage T4. Nature (London) 227:680-685.
    • (1970) Nature (London) , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 28
    • 0025890863 scopus 로고
    • Purification of a sugar beet pectin modifying enzyme system from Aspergillus niger by antibody affinity column chromatography
    • Matthew, J. A., G. M. Wyatt, D. B. Archer, and M. R. A. Morgan. 1991. Purification of a sugar beet pectin modifying enzyme system from Aspergillus niger by antibody affinity column chromatography. Carbohydr. Polym. 16: 381-390.
    • (1991) Carbohydr. Polym. , vol.16 , pp. 381-390
    • Matthew, J.A.1    Wyatt, G.M.2    Archer, D.B.3    Morgan, M.R.A.4
  • 29
    • 0000612787 scopus 로고
    • Comparison of endolytic hydrolases that depolymerize 1,4-β-D-mannan, 1,5-α-L-arabinan, and 1,4-β-D-galactan
    • G. F. Leatham and M. E. Himmel (ed.), American Chemical Society, Washington, D.C.
    • McCleary, B. V. 1991. Comparison of endolytic hydrolases that depolymerize 1,4-β-D-mannan, 1,5-α-L-arabinan, and 1,4-β-D-galactan, p. 437-449. In G. F. Leatham and M. E. Himmel (ed.), Enzymes in biomass conversion. American Chemical Society, Washington, D.C.
    • (1991) Enzymes in Biomass Conversion , pp. 437-449
    • McCleary, B.V.1
  • 30
    • 0027423432 scopus 로고
    • Arabinan degrading enzymes from Aspergillus nidulans: Induction and purification
    • Ramón, D., P. van der Veen, and J. Visser. 1993. Arabinan degrading enzymes from Aspergillus nidulans: induction and purification. FEMS Microbiol. Lett. 113:15-22.
    • (1993) FEMS Microbiol. Lett. , vol.113 , pp. 15-22
    • Ramón, D.1    Van Der Veen, P.2    Visser, J.3
  • 31
    • 0002816842 scopus 로고
    • The arabinanases of Aspergillus niger - Purification and characterisation of two α-L-arabinofuranosidases and an endo-1,5-α-L-arabinanase
    • Rombouts, F. M., A. G. Voragen, M. F. Searl-van Leeuwen, C. C. J. M. Geraeds, H. A. Schols, and W. Plinik. 1988. The arabinanases of Aspergillus niger - purification and characterisation of two α-L-arabinofuranosidases and an endo-1,5-α-L-arabinanase. Carbohydr. Polym. 9:25-47.
    • (1988) Carbohydr. Polym. , vol.9 , pp. 25-47
    • Rombouts, F.M.1    Voragen, A.G.2    Searl-van Leeuwen, M.F.3    Geraeds, C.C.J.M.4    Schols, H.A.5    Plinik, W.6
  • 32
    • 0030561471 scopus 로고    scopus 로고
    • Expression in a wine yeast strain of the Aspergillus niger abfB gene
    • Sanchez-Torres, P., L. Gonzalez-Candelas, and D. Ramon. 1996. Expression in a wine yeast strain of the Aspergillus niger abfB gene. FEMS Microbiol. Lett. 145:189-194.
    • (1996) FEMS Microbiol. Lett. , vol.145 , pp. 189-194
    • Sanchez-Torres, P.1    Gonzalez-Candelas, L.2    Ramon, D.3
  • 33
    • 0029822445 scopus 로고    scopus 로고
    • L-Arabinose selectively inhibits intestinal sucrase in an uncompetitive manner and reduces glycaemic response after sucrose ingestion in animals
    • Seri, K., K. Sanai, N. Matsuo, K. Kawakubo, C.-Y. Xue, and S. Inoue. 1996. L-Arabinose selectively inhibits intestinal sucrase in an uncompetitive manner and reduces glycaemic response after sucrose ingestion in animals. Metabolism 45:1368-1374.
    • (1996) Metabolism , vol.45 , pp. 1368-1374
    • Seri, K.1    Sanai, K.2    Matsuo, N.3    Kawakubo, K.4    Xue, C.-Y.5    Inoue, S.6
  • 35
    • 33750423631 scopus 로고
    • Notes on sugar determination
    • Somogyi, M. 1952. Notes on sugar determination. J. Biol. Chem. 195:19-23.
    • (1952) J. Biol. Chem. , vol.195 , pp. 19-23
    • Somogyi, M.1
  • 36
    • 0344466983 scopus 로고
    • Kinetic studies of α-L-arabinofuranosidase from Aspergillus niger. I. General kinetic properties of the enzyme
    • Tagawa, K. 1970. Kinetic studies of α-L-arabinofuranosidase from Aspergillus niger. I. General kinetic properties of the enzyme. J. Ferment. Technol. 48: 730-739.
    • (1970) J. Ferment. Technol. , vol.48 , pp. 730-739
    • Tagawa, K.1
  • 37
    • 0344466982 scopus 로고
    • Kinetic studies of α-L-arabinofuranosidase from Aspergillus niger. II. Stability of the enzyme
    • Tagawa, K. 1970. Kinetic studies of α-L-arabinofuranosidase from Aspergillus niger. II. Stability of the enzyme. J. Ferment. Technol. 48:740-746.
    • (1970) J. Ferment. Technol. , vol.48 , pp. 740-746
    • Tagawa, K.1
  • 38
    • 49849127595 scopus 로고
    • Preparation of L-arabinose-containing polysaccharides and the action of an α-L-arabinofuranosidase on these polysaccharides
    • Tagawa, K., and A. Kaji. 1969. Preparation of L-arabinose-containing polysaccharides and the action of an α-L-arabinofuranosidase on these polysaccharides. Carbohydr. Res. 11:293-301.
    • (1969) Carbohydr. Res. , vol.11 , pp. 293-301
    • Tagawa, K.1    Kaji, A.2
  • 39
    • 0013493859 scopus 로고
    • Studies on the enzymes acting on araban. IX. Culture conditions and process of arabanase production by Aspergillus niger
    • Tagawa, K., and G. Terui. 1968. Studies on the enzymes acting on araban. IX. Culture conditions and process of arabanase production by Aspergillus niger. J. Ferment. Technol. 46:693-700.
    • (1968) J. Ferment. Technol. , vol.46 , pp. 693-700
    • Tagawa, K.1    Terui, G.2
  • 40
    • 0019879662 scopus 로고
    • Substrate specificity of α-L-arabinofuranosidase from plant Scopolia japonica calluses and a suggestion with reference to the structure of beet arabinan
    • Tanaka, M., A. Abe, and T. Uchida. 1981. Substrate specificity of α-L-arabinofuranosidase from plant Scopolia japonica calluses and a suggestion with reference to the structure of beet arabinan. Biochim. Biophys. Acta 658:377-386.
    • (1981) Biochim. Biophys. Acta , vol.658 , pp. 377-386
    • Tanaka, M.1    Abe, A.2    Uchida, T.3
  • 41
    • 0028598691 scopus 로고
    • Extracellular arabinases in Aspergillus nidulans: The effect of different ere mutations on enzyme levels
    • van der Veen, P., H. N. Arst, Jr., M. J. A. Flipphi, and J. Visser. 1994. Extracellular arabinases in Aspergillus nidulans: the effect of different ere mutations on enzyme levels. Arch. Microbiol. 162:433-440.
    • (1994) Arch. Microbiol. , vol.162 , pp. 433-440
    • Van Der Veen, P.1    Arst H.N., Jr.2    Flipphi, M.J.A.3    Visser, J.4
  • 42
    • 0025987758 scopus 로고
    • Induction, purification and characterization of arabinanases produced by Aspergillus niger
    • van der Veen, P., M. J. A. Flipphi, A. G. J. Voragen, and J. Visser. 1991. Induction, purification and characterization of arabinanases produced by Aspergillus niger. Arch. Microbiol. 157:23-28.
    • (1991) Arch. Microbiol. , vol.157 , pp. 23-28
    • Van Der Veen, P.1    Flipphi, M.J.A.2    Voragen, A.G.J.3    Visser, J.4
  • 43
    • 0027264360 scopus 로고
    • Induction of extracellular arabinases on monomeric substrates in Aspergillus niger
    • van der Veen, P., M. J. A. Flipphi, A. G. J. Voragen, and J. Visser. 1993. Induction of extracellular arabinases on monomeric substrates in Aspergillus niger. Arch. Microbiol. 159:66-71.
    • (1993) Arch. Microbiol. , vol.159 , pp. 66-71
    • Van Der Veen, P.1    Flipphi, M.J.A.2    Voragen, A.G.J.3    Visser, J.4
  • 44
    • 0000113565 scopus 로고
    • Determination, by methylation analysis, of the glycosyl-linkage compositions of microgram quantities of complex carbohydrates
    • Waeghe, T. J., A. G. Darvill, M. McNeil, and P. Albersheim. 1983. Determination, by methylation analysis, of the glycosyl-linkage compositions of microgram quantities of complex carbohydrates. Carbohydr. Res. 123:281-304.
    • (1983) Carbohydr. Res. , vol.123 , pp. 281-304
    • Waeghe, T.J.1    Darvill, A.G.2    McNeil, M.3    Albersheim, P.4
  • 45
    • 0001412964 scopus 로고
    • Structure of plant cell walls. IX. Purification and partial characterization of a wall degrading endoarabanase and arabinosidase from Bacillus subtilis
    • Weinstein, L., and P. Albersheim. 1979. Structure of plant cell walls. IX. Purification and partial characterization of a wall degrading endoarabanase and arabinosidase from Bacillus subtilis. Plant Physiol. 63:425-432.
    • (1979) Plant Physiol. , vol.63 , pp. 425-432
    • Weinstein, L.1    Albersheim, P.2
  • 46
    • 0029984854 scopus 로고    scopus 로고
    • Arabinoxylan-degrading enzyme system of the fungus Aspergillus awamori: Purification and properties of an alpha-L-arabinofuranosidase
    • Wood, T. M., and S. I. McCrae. 1996. Arabinoxylan-degrading enzyme system of the fungus Aspergillus awamori: purification and properties of an alpha-L-arabinofuranosidase. Appl. Microbiol. Biotechnol. 45:538-545.
    • (1996) Appl. Microbiol. Biotechnol. , vol.45 , pp. 538-545
    • Wood, T.M.1    McCrae, S.I.2
  • 47
    • 77957029755 scopus 로고
    • Isolation and characterization of plant cell walls and cell wall components
    • York, W. S., A. G. Darvill, M. McNeil, and P. Albersheim. 1985. Isolation and characterization of plant cell walls and cell wall components. Methods Enzymol. 118:3-40.
    • (1985) Methods Enzymol. , vol.118 , pp. 3-40
    • York, W.S.1    Darvill, A.G.2    McNeil, M.3    Albersheim, P.4
  • 48
    • 0344035847 scopus 로고
    • Detection of α-L-arabinofuranosidase activity in isoelectric focused gels using 6-bromo-2-naphthyl-α-L-arabinofuranoside
    • Yoshida, S., Y. Kawabata, S. Kaneko, Y. Nagamoto, and I. Kusakabe. 1994. Detection of α-L-arabinofuranosidase activity in isoelectric focused gels using 6-bromo-2-naphthyl-α-L-arabinofuranoside. Biosci. Biotechnol. Biochem. 58:580-581.
    • (1994) Biosci. Biotechnol. Biochem. , vol.58 , pp. 580-581
    • Yoshida, S.1    Kawabata, Y.2    Kaneko, S.3    Nagamoto, Y.4    Kusakabe, I.5
  • 49
    • 0025381008 scopus 로고
    • Structure of rice-straw arabinoglucuronoxylan and specificity of Streptomyces xylanase toward the xylan
    • Yoshida, S., I. Kusakabe, N. Matsuo, K. Shimizu, T. Yasui, and K. Murakami. 1990. Structure of rice-straw arabinoglucuronoxylan and specificity of Streptomyces xylanase toward the xylan. Agric. Biol. Chem. 54:449-457.
    • (1990) Agric. Biol. Chem. , vol.54 , pp. 449-457
    • Yoshida, S.1    Kusakabe, I.2    Matsuo, N.3    Shimizu, K.4    Yasui, T.5    Murakami, K.6


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