Deletion of the alternative sigma factor σ(B) in Staphylococcus aureus reveals its function as a global regulator of virulence genes

Journal of Bacteriology

Volumn 180, Issue 18, 1998, Pages 4814-4820

  Kullik, Ines ^a,b   Giachino, Philipp ^a   Fuchs, Thomas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b Pharmacia and Upjohn AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CYTOPLASM PROTEIN; NUCLEASE; SIGMA FACTOR;

ARTICLE; BACTERIAL GENETICS; BACTERIAL GROWTH; BACTERIAL VIRULENCE; BACTERIUM MUTANT; GENE DELETION; OPERON; PRIORITY JOURNAL; PROTEIN EXPRESSION; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 0031659829     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.18.4814-4820.1998     Document Type: Article

References (36)

1. Ausubel, F. M., R. Brent, R. F. Kingston, D. D. Moore, J. G. Seidmann, J. A. Smith, and K. Struhl. 1987. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
2. Cheung, A. L., J. M. Koomey, C. A. Butler, S. J. Projan, and V. A. Fischetti. 1992. Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr. Proc. Natl. Acad. Sci. USA 89:6462-6466.
3. Cheung, A. L., and S. J. Projan. 1994. Cloning and sequencing of sarA of Staphylococcus aureus, a gene required for the expression of agr. J. Bacteriol. 176:4168-4172.
4. Cheung, A. L., and P. Ying. 1994. Regulation of α-and β-hemolysins by the sar locus of Staphylococcus aureus. J. Bacteriol. 176:580-585.
5. Cone, J. L., C. L. Cusumano, H. Taniuchi, and C. B. Anfinsen. 1971. Staphylococcal nuclease (Foggi strain). II. The amino acid sequence. J. Biol. Chem. 246:3103-3110.
6. Davis, A., I. B. Moore, D. S. Parker, and H. Taniuchi. 1977. Nuclease B. A possible precursor of nuclease A, an extracellular nuclease of Staphylococcus aureus. J. Biol. Chem. 252:6544-6553.
7. Degteva, G. K., and T. A. Malakhova. 1979. Quantitative indices of the activity and the electrophoretic characteristics of staphylococcal catalase. Zh. Mikrobiol. Epidemiol. Immunobiol. 6:31-34.
8. SB of Staphylococcus aureus: characterization and role in transcription of the global regulatory locus sar. J. Bacteriol. 179:6355-6359.
9. Duthie, E. S., and L. L. Lorenz. 1952. Staphylococcal coagulase: mode of action and antigenicity. J. Gen. Microbiol. 6:95-107.
10. B-dependent catalase in Bacillus subtilis. J. Bacteriol. 177:5598-5605.
11. Foster, T. J., and D. McDevitt. 1994. Surface-associated proteins of Staphylococcus aureus: their possible roles in virulence. FEMS Microbiol. Lett. 118:199-205.
12. Francois, P., P. Vaudaux, T. J. Foster, and D. P. Lew. 1996. Host-bacteria interactions in foreign body infections. Infect. Control Hosp. Epidemiol. 17:514-520.
13. Götz, F., and B. Schumacher. 1987. Improvements of protoplast transformation in Staphylococcus aureus. FEMS Microbiol. Lett. 40:285-288.
14. Hecker, M., W. Schumann, and U. Voelker. 1996. Heat-shock and general stress response in Bacillus subtilis. Mol. Microbiol. 19:417-428.
15. Ivanova, A., C. Miller, G. Glinsky, and A. Eisenstark. 1994. Role of rpoS (katF) in OxyR-independent regulation of hydroperoxidase I in Escherichia coli. Mol. Microbiol. 12:571-578.
16. Kanafani, H., and S. E. Martin. 1985. Catalase and superoxide dismutase activities in virulent and nonvirulent Staphylococcus aureus isolates. J. Clin. Microbiol. 21:607-610.
17. Kato, F., T. Hino, A. Nakaji, M. Tanaka, and Y. Koyama. 1995. Carotenoid synthesis in Streptomyces setonii ISP5395 is induced by the gene crtS, whose product is similar to a sigma factor. Mol. Gen. Genet. 247:387-390.
18. Kovacevic, S., L. E. Veal, H. M. Hsiung, and J. R. Miller. 1985. Secretion of staphylococcal nuclease by Bacillus subtilis. J. Bacteriol. 162:521-528.
19. Kreiswirth, B. N., S. Löfdahl, M. J. Betley, M. O'Reilly, P. M. Schlievert, M. S. Bergdoll, and R. P. Novick. 1983. The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305:709-712.
20. B in Staphylococcus aureus: regulation of the sigB operon in response to growth phase and heat shock. Arch. Microbiol. 167:151-159.
21. Kuroda, M., T. Ohta, and H. Hayashi. 1995. Isolation and the gene cloning of an alkaline shock protein in methicillin resistant Staphylococcus aureus. Biochem. Biophys. Res. Commun. 207:978-984.
22. Mandell, G. L. 1975. Catalase, superoxide dismutase, and virulence of Staphylococcus aureus. In vitro and in vivo studies with emphasis on staphylococcal-leucocyte interaction. J. Clin. Invest. 55:561-566.
23. McDevitt, D., P. Francois, P. Vaudaux, and T. J. Foster. 1994. Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus. Mol. Microbiol. 11:237-248.
24. Mekalanos, J. J. 1992. Environmental signals controlling expression of virulence determinants in bacteria. J. Bacteriol. 174:1-7.
25. Mukhopadhyay, S., and H. E. Schellhorn. 1994. Induction of Escherichia coli hydroperoxidase I by acetate and other weak acids. J. Bacteriol. 176:2300-2307.
26. National Committee for Clinical Laboratory Standards. 1997. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A4. National Committee for Clinical Laboratory Standards, Villanova, Pa.
27. Peschel, A., J. Augustin, T. Kupke, S. Stevanovic, and F. Götz. 1993. Regulation of epidermin biosynthetic genes by EpiQ. Mol. Microbiol. 9:31-39.
28. Peschel, A., B. Ottenwälder, and F. Götz. 1996. Inducible production and cellular location of the epidermin biosynthetic enzyme EpiQ using an improved staphylococcal expression system. FEMS Microbiol. Lett. 137:279-284.
29. Projan, S. J., and R. P. Novick. 1997. The molecular basis of pathogenicity, p. 55-81. In K. B. Crossley and G. L. Archer (ed.), The staphylococci in human diseases. Churchill Livingstone, New York, N.Y.
30. Recsei, P., B. Kreiswirth, M. O'Reilly, P. Schlievert, A. Gruss, and R. P. Novick. 1986. Regulation of exoprotein gene expression in Staphylococcus aureus by AgaR. Mol. Gen. Genet. 202:58-61.
31. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
32. B. J. Bacteriol. 177:114-122.
33. B of Bacillus subtilis in response to environmental and metabolic stresses. J. Bacteriol. 177:3771-3780.
34. Wieland, B., C. Feil, E. Gloria-Maerker, G. Thumm, M. Lechner, J.-M. Bravo, K. Poralla, and F. Götz. 1994. Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4′-diaponeurosporene of Staphylococcus aureus. J. Bacteriol. 176:7719-7726.
35. Wieland, K. P., and F. Götz. 1996. Unpublished data.
36. Wu, S., H. de Lencastre, and A. Tomasz. 1996. Sigma-B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase: molecular cloning and DNA sequencing. J. Bacteriol. 178:6036-6042.