Point mutations in the WD40 domain of Eed block its interaction with Ezh2

Molecular and Cellular Biology

Volumn 18, Issue 10, 1998, Pages 5634-5642

  Denisenko, Oleg ^a   Shnyreva, Maria ^a   Suzuki, Hideaki ^a   Bomsztyk, Karol ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; COMPLEX FORMATION; DNA LIBRARY; GENE EXPRESSION REGULATION; GENETIC REGULATION; MORPHOGENESIS; MULTIGENE FAMILY; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN QUATERNARY STRUCTURE; RAT; TRANSCRIPTION REGULATION;

ANIMALIA; MAMMALIA; MURINAE;

EID: 0031658231     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.18.10.5634     Document Type: Article

References (59)

1. Abel, K. J., L. C. Brody, J. M. Vlades, M. R. Erdos, D. R. McKinley, L. H. Castilla, S. D. Merajver, F. J. Couch, L. S. Friedman, E. A. Ostermeyer, E. D. Lynch, M.-C. King, P. L. Welcsh, S. Osborne-Lawrence, M. Spillman, A. M. Bowcock, F. S. Collins, and B. L. Weber. 1996. Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1. Genomics 37:161-171.
2. Bird, T. A., H. Schule, P. B. Delaney, J. E. Sims, B. Thoma, and S. K. Dower. 1992. Evidence that MAP (mitogen-activated protein) kinase activation may be a necessary but not sufficient signal for a restricted subset of responses in IL-1-treated epidermoid cells. Cytokine 4:429-440.
3. Bomsztyk, K., I. Van Seuningen, H. Suzuki, O. Denisenko, and J. Ostrowski. 1997. Diverse molecular interactions of the hnRNP K protein. FEBS Lett. 403:113-115.
4. Burd, C. G., and G. Dreyfuss. 1994. Conserved structures and diversity of functions of RNA-binding proteins. Science 265:615-621.
5. Carrington, E. A., and R. S. Jones. 1996. The Drosophila Enhancer of zeste gene encodes a chromatin protein: examination of wild-type and mutant protein distribution. Development 122:4073-4083.
6. Chen, H., C. Rossier, and S. E. Antonarakis. 1996. Cloning of a human homolog of the Drosophila Enhancer of zeste gene (EZH2) that maps to the chromosome 21q22.2. Genomics 38:30-37.
7. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
8. Denisenko, O. N., and K. Bomsztyk. 1997. The product of the murine homolog of the Drosophila extra sex comb gene displays transcriptional repressor activity. Mol. Cell. Biol. 17:4707-4717.
9. 8a. Denisenko, O. N., and K. Bomsztyk. Unpublished data.
10. Eissenberg, J. C., T. C. James, D. M. Foster-Harnett, T. Harnett, T. Ngan, and S. C. R. Elgin. 1990. Mutation in a heterochromatin-specific chromosomal protein is associated with suppression of position-effect variegation in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 87:9923-9927.
11. Fields, S., and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340:245-246.
12. Gatti, M., and B. S. Baker. 1989. Genes controlling essential cell-cycle functions in Drosophila melanogaster. Genes Dev. 3:438-453.
13. Gillis, S., and J. Watson. 1980. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J. Exp. Med. 152:1709-1719.
14. Gunster, M., D. Satijn, K. Hamer, J. den Blaauwen, D. de Bruijn, M. Alkema, M. van Lohuizen, R. van Driel, and A. Otte. 1997. Identification and characterization of interactions between the vertebrate polycomb-group protein BMI1 and human homologs of polyhomeotic. Mol. Cell. Biol. 17:2326-2335.
15. Gurevich, V. V., I. D. Pokrovskaya, T. A. Obukhova, and S. A. Zozulya. 1991. Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerase. Anal. Biochem. 195:207-213.
16. Gutjahr, T., E. Frei, C. Spicer, S. Baumgartner, R. A. H. White, and M. Noll. 1995. The polycomb-group gene, extra sex combs, encodes a nuclear member of the WD-40 repeat family. EMBO J. 14:4296-4306.
17. Hubert, O., I. Sures, T. Ciossek, M. Fuchs, and A. Ullrich. 1996. Isolation and developmental expression analysis of Enx-1, a novel mouse Polycomb group gene. Mech. Dev. 55:171-184.
18. Ito, N., S. E. Phillips, C. Stevens, Z. B. Ogel, M. J. McPherson, J. N. Keen, K. D. Yadav, and P. F. Knowles. 1991. Novel thioether bond revealed by a 1.7 A crystal structure of galactose oxidase. Nature 350:87-90.
19. Johnson, R. J., H. Iida, C. E. Alpers, M. W. Majesky, S. M. Schwartz, P. Pritzl, K. Gordon, and A. M. Gwon. 1991. Expression of smooth muscle cell phenotype by rat mesangial cells in immune complex nephritis: α-smooth muscle actin is a marker of mesangial cell proliferation. J. Clin. Invest. 87:847-858.
20. Jones, C. A., J. Ng, A. J. Peterson, K. Morgan, J. Simon, and R. S. Jones. 1998. The Drosophila esc and E(z) proteins are direct partners in polycomb group-mediated repression. Mol. Cell. Biol. 18:2825-2834.
21. Jones, R. S., and W. M. Gelbart. 1993. The Drosophila Polycomb-group gene Enhancer of zeste contains a region with sequence similarity to trithorax. Mol. Cell. Biol. 13:6357-6366.
22. Jones, R. S., and W. M. Gelbart. 1990. Genetic analysis of the enhancer of zeste locus and its role in gene regulation in Drosophila melanogaster. Genetics 126:185-199.
23. Kalisch, W. E., and B. Rasmuson. 1974. Changes of zeste phenotype induced by autosomal mutations in Drosophila melanogaster. Hereditas 78:97-104.
24. Kluxen, F. W., and H. Lubbert. 1993. Maximal expression of recombinant cDNAs in COS cells for use in expression cloning. Anal. Biochem. 208:352-356.
25. Laible, G., A. Wolf, R. Dorn, G. Reuter, C. Nislow, A. Lebersorger, D. Popkin, L. Pillus, and T. Jenuwein. 1997. Mammalian homologues of the Polycomb-group gene Enhancer of zeste mediate gene silencing in Drosophila heterochromatin and at S. cerevisiae telomeres. EMBO J. 16:3219-3232.
26. LaJeunesse, D., and A. Shearn. 1996. E(z): a polycomb group gene or a trithorax group gene? Development 122:2189-2197.
27. Lambright, D. G., J. Sondek, A. Bohm, N. P. Skiba, H. E. Hamm, and P. B. Sigler. 1996. The 2.0 A crystal structure of a heterotrimeric G protein. Nature 379:311-319.
28. Locke, J., M. A. Kotarski, and K. D. Tartof. 1988. Dosage-dependent modofiers of position effect variegation in Drosophila and a mass action model that explains their effect. Genetics 120:181-198.
29. McCall, K., and W. Bender. 1996. Probes for chromatin accessibility in the Drosophila bithorax complex respond differently to Pofycomb-mediated repression. EMBO J. 15:569-580.
30. Ng, J., R. Li, K. Morgan, and J. Simon. 1997. Evolutionary conservation and predicted structure of the Drosophila extra sex combs repressor protein. Mol. Cell. Biol. 17:6663-6672.
31. Paige, C. J., P. W. Kincaide, and P. Ralph. 1978. Murine B cell leukemia line with inducible surface immunoglobulin expression. J. Immunol. 121:641-647.
32. Paro, R. 1990. Imprinting the determined state into the chromatin of Drosophila melanogaster. Trends Genet. 6:416-421.
33. Paro, R., S. Messmer, Moehrle, V. Orlando, and D. Zink. 1993. Regulation of stable gene expression at the higher-order chromatin level. Genetics and the Understanding of Life. 17th International Congress of Genetics, Birmingham, United Kingdom.
34. Phillips, M. D., and A. Shearn. 1990. Mutations in polycombeotic, a Drosophila polycomb-group gene, cause a wide range of maternal and zygotic phenotypes. Genetics 125:91-101.
35. Pirrotta, V. 1995. Chromatin complexes regulating gene expression in Drosophila. Curr. Opin. Genet. Dev. 5:466-472.
36. Pirrotta, V. 1997. PcG complexes and chromatin silencing. Curr. Opin. Genet. Dev. 7:249-258.
37. Platero, J. S., E. J. Sharp, P. N. Adler, and J. C. Eissenberg. 1996. In vivo assay for protein-protein interactions using Drosophila chromosomes. Chromosoma 104:393-404.
38. Rachie, N. A., R. Seger, M. A. Valentine, J. Ostrowski, and K. Bomsztyk. 1993. Identification of an inducible 85-kDa nuclear protein kinase. J. Biol. Chem. 268:22143-22149.
39. Rastelli, L., C. S. Chen, and V. Pirrotta. 1993. Related chromosome binding sites for zeste, suppressors of zeste and Polycomb group proteins in Drosophila and their dependence on Enhancer of zeste function. EMBO J. 12:1513-1522.
40. Reuter, G., M. Giarre, J. Farah, J. Gausz, A. Spierer, and P. Spierer. 1990. Dependence of position-effect variegation in Drosophila on dose of a gene encoding an unusual zinc-finger protein. Nature 344:219-223.
41. Reuter, G., and P. Spierer. 1992. Position effect variegation and chromatin structure. Bioessays 14:605-612.
42. Richardson, C. A., K. L. Gordon, W. G. Couser, and K. Bomsztyk. 1995. IL-1β increases laminin B2 chain mRNA levels and activates NF-κB in rat glomerular epithelial cells. Am. J. Physiol. 268: F273-F278.
43. Sadowski, I., and M. Ptashne. 1989. A vector for expressing GAL4 (1-147) fusions in mammalian cells. Nucleic Acids Res. 17:7539.
44. Sathe, S. S., and P. J. Harte. 1995. The Drosophila extra sex combs protein contains WD motifs essential for its function as a repressor of homeotic genes. Mech. Dev. 52:77-87.
45. Sathe, S. S., and P. J. Harte. 1995. The extra sex combs protein is highly conserved between Drosophila virilis and Drosophila melanogaster. Mech. Dev. 52:225-232.
46. Schumacher, A., C. Faust, and T. Magnuson. 1996. Positional cloning of a global regulator of anterior-posterior patterning in mice. Nature 383:250-253.
47. Sewalt, R. G. A. B., J. van der Vlag, M. J. Gunster, K. M. Hamer, J. L. den Blaauwen, D. P. E. Satijn, T. Hendrix, R. van Driel, and A. P. Otte. 1998. Characterization of interaction between the mammalian Polycomb-group proteins Enx1/Ezh2 and Eed suggests the existence of different mammalian Polycomb-group protein complexes. Mol. Cell. Biol. 18:3586-3595.
48. Simon, J. 1995. Locking in stable states of gene expression: transcriptional control during Drosophila development. Curr. Opin. Cell Biol. 7:376-385.
49. Simon, J., D. Bornemann, K. Lunde, and C. Schwartz. 1995. The extra sex combs product contains WD40 repeats and its time of action implies a role distinct from other Polycomb group products. Mech. Dev. 53:197-208.
50. Simon, J., A. Chiang, W. Bender, M. J. Shimell, and M. O'Connor. 1993. Elements of the Drosophila bithorax complex that mediate repression by Polycomb group products. Development 158:131-144.
51. Singh, P. B. 1994. Molecular mechanisms of cellular determination: their relation to chromatin structure and parental imprinting. J. Cell Sci. 107: 2653-2668.
52. Struhl, G. 1981. A gene product required for correct initiation of segmental determination in Drosophila. Nature 293:36-41.
53. + gene product in ensuring the selective expression of segment-specific homeotic genes in Drosophila. J. Embryol. Exp. Morphol. 76:297-331.
54. + gene product in the determination of segments in Drosophila. Cell 31:285-292.
55. Tschiersch, B., A. Hofmann, V. Krauss, R. Dorn, G. Korge, and G. Reuter. 1994. The protein encoded by the Drosophila position-effect variegation suppressor gene Su(var)3-9 combines domains of antagonistic regulators of homeotic gene complexes, EMBO J. 13:3822-3831.
56. van Lohuizen, M., M. Tijms, J. W. Voncken, A. Schumacher, T. Magnuson, and E. Wientjens. 1998. Interaction of mouse Polycomb-group (PcG) proteins Enx1 and Enx2 with Eed: indication for separate PcG complexes. Mol. Cell. Biol. 18:3572-3579.
57. Vojtek, A. B., S. M. Hollenberg, and J. A. Cooper. 1993. Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell 74:205-214.
58. White, R. A. H., and R. Lehmann. 1986. A gap gene, hunchback, regulates the spatial expression of Ultrabithorax. Cell 47:311-321.
59. Zhang, C.-C., and M. Bienz. 1992. Segmental determination in Drosophila conferred by hunchback (hb), a repressor of the homeotic gene Ultrabothorax (Ubx). Proc Natl. Acad. Sci. USA 89:7511-7515.