In vitro evidence that rabbit distal colonic muscularis mucosae has a Clostridium difficile toxin A receptor

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 275, Issue 3 38-3, 1998, Pages

  Percy, W H ^a   Burakoff, R ^b,c   Rose, K ^b,c   Desai, H P ^b,c   Pothoulakis, C ^d   Eglow, R ^b,c  

^a UNIVERSITY OF SOUTH DAKOTA   (United States)

^b WINTHROP UNIVERSITY HOSPITAL   (United States)

^c STONY BROOK UNIVERSITY   (United States)

^d University Hospital   (United States)

Author keywords

Antibiotic associated colitis; Circular muscle; Contraction; Immunohistochemistry; Lectin; Longitudinal muscle

Indexed keywords

ACETYLCHOLINE; ATROPINE; CELL RECEPTOR; CLOSTRIDIUM DIFFICILE TOXIN A; INDOMETACIN; LECTIN; POTASSIUM ION; TETRODOTOXIN;

ANIMAL TISSUE; ARTICLE; BACTERIAL OVERGROWTH; BACTERIUM ADHERENCE; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; DESCENDING COLON; IMMUNOHISTOCHEMISTRY; INTESTINE MUSCLE; MALE; MUSCLE CONTRACTION; NONHUMAN; PRIORITY JOURNAL; PSEUDOMEMBRANOUS COLITIS; RABBIT;

EID: 0031657156     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1998.275.3.g402     Document Type: Article

References (35)

1. Bartlett, J. G., N. Moon, T. W. Chang, N. Taylor, and A. B. Onderdonk. Role of Clostridium difficile in antibiotic-associated colitis. Gastroenterology 75: 778-782, 1978.
2. Burakoff, R., L. Zhao, A. J. Celifarco, K. L. Rose, V. Donovan, C. Pothoulakis, and W. H. Percy. Effects of purified Clostridium difficile toxin A on rabbit distal colon. Gastroenterology 109: 348-354, 1995.
3. Castagliuolo, I., J. T. LaMont, R. Letourneau, C. Kelly, J. C. O'Keane, A. Jaffer, T. C. Theoharides, and C. Pothoulakis. Neuronal involvement in the intestinal effects of Clostridium difficile toxin A and Vibrio cholerae enterotoxin in rat ileum. Gastroenterology 107: 657-665, 1994.
4. Castagliuolo, I., J. T. LaMont, B. S. Qiu, S. T. Nikulasson, and C. Pothoulakis. A receptor decoy inhibits the enterotoxic effects of Clostridium difficile toxin Ain rat ileum. Gastroenterology 111: 433-438, 1996.
5. Desai, H., J. Goldhill, and R. Burakoff. C. difficile toxin A causes fluid secretion in the rabbit distal colon in vivo without neutrophil infiltration (Abstract). Gastroenterology 104: A690, 1993.
6. Eglow, R., C. Pothoulakis, S. Itzkowitz, E. J. Israel, C. J. O'Keane, D. Dahai, N. Gao, Y. L. Xu, W. A. Walker, and J. T. LaMont. Diminished Clostridium difficile toxin A sensitivity in newborn rabbit ileum is associated with decreased toxin A receptor. J. Clin. Invest. 90: 822-829, 1992.
7. Gilbert, R. J., C. Pothoulakis, and J. T. LaMont. Effect of purified Clostridium difficile toxins on intestinal smooth muscle. II. Toxin B. Am. J. Physiol. 256 (Gastrointest. Liver Physiol. 19): G767-G772, 1989.
8. Gilbert, R. J., C. Pothoulakis, J. T. LaMont, and M. Yakubovich. Clostridium difficile toxin B activates calcium influx required for actin disassembly during cytotoxicity. Am. J. Physiol. 268 (Gastrointest. Liver Physiol. 31): G487-G495, 1995.
9. Gilbert, R. J., G. Triadafilopoulos, C. Pothoulakis, C. Giampolo, and J. T. LaMont. Effect of purified Clostridium difficile toxins on intestinal smooth muscle. I. Toxin A. Am. J. Physiol. 256 (Gastrointest Liver Physiol. 19): G759-G766, 1989.
10. Goldhill, J. M., R. Burakoff, V. Donovan, K. Rose, and W. H. Percy. Defective modulation of colonic secretomotor neurons in a rabbit model of colitis. Am. J. Physiol. 264 (Gastrointest. Liver Physiol. 27): G671-G677, 1993.
11. Hecht, G., A. Koutsouris, C. Pothoulakis, J. T. Lamont, and J. L. Madara. Clostridium difficile toxin B disrupts the barrier function of T84 monolayers. Gastroenterology 102: 416-423, 1992.
12. Hecht, G., C. Pothoulakis, J. T. LaMont, and J. Madara. Clostridium difficile toxin A perturbs cytoskeletal structure and tight junction permeability of cultured human intestinal epithelial monolayers. J. Clin. Invest. 82: 1516-1524, 1988.
13. Hnatowitch, D. J., F. Virzi, and M. Rusckowski. Investigations of avidin and biotin for imaging applications. J. Nucl. Med. 28: 1294-1302, 1987.
14. Justus, P. G., J. L. Martin, D. A. Goldberg, N. S. Taylor, J. G. Bartlett, R. W. Alexander, and J. R. Mathias. Myoelectric effects of Clostridium difficile: motility-altering factors distinct from its cytotoxin and enterotoxin in rabbits. Gastroenterology 83: 836-843, 1982.
15. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970.
16. Lawson, L. D., and D. W. Powell. Bradykinin-stimulated eicosanoid synthesis and secretion by rabbit ileal components. Am. J. Physiol. 252 (Gastrointest. Liver Physiol. 15): G783-G790, 1987.
17. Lima, A. A. M., D. M. Lyerly, T. D. Wilkins, D. J. Innes, and R. L. Guerrant. Effects of Clostridium difficile toxins A and B on rabbit small and large intestine in vivo and on cultured cells in vitro. Infect. Immun. 56: 582-588, 1988.
18. Lyerly, D. M., D. E. Lockwood, S. H. Richardson, and T. D. Wilkins. Biological activities of toxins A and B of Clostridium difficile. Infect. Immun. 35: 1147-1150, 1982.
19. Mathias, J. R., J. Nogueira, J. L. Martin, G. M. Carlson, and R. A. Giannella. Escherichia coli heat stable toxin: its effect on motility of the small intestine. Am. J. Physiol. 242 (Gastrointest. Liver Physiol. 5): G360-G363, 1982.
20. Mitchell, T. J., J. M. Ketley, S. C. Haslam, J. Stephen, D. W. Burdon, D. C. W. Candy, and R. Daniel. Effect of toxin A and B of Clostridium difficile on rabbit ileum and colon. Gut 27: 78-85, 1986.
21. Percy, W. H., M. B. Burton, F. Fallick, and R. Burakoff. A comparison in vitro of human and rabbit distal colonic muscle responses to inflammatory mediators. Gastroenterology 99: 1324-1332, 1990.
22. Percy, W. H., M. B. Burton, F. Fallick, and R. Burakoff. Rat colonie motor responses to inflammatory mediators in vitro: a poor model for the human colon. J. Gastrointest. Motil. 3: 229-236, 1991.
23. Percy, W. H., M. B. Burton, W. R. Glaws, K. Rose, and R. Burakoff. Pharmacological basis of contractile effects of peptido-leukotrienes on rabbit colonie muscularis mucosae. Am. J. Physiol. 264 (Gastrointest. Liver Physiol. 27): G81-G85, 1993.
24. Percy, W. H., M. B. Burton, Y. Jacobowitz, and R. Burakoff. An investigation in vitro of the properties of the individual muscle layers of the rabbit colon in an induced colitis. In: Effects of Immune Cells and Inflammation on Smooth Muscle and Enteric Nerves, edited by W. J. Snape and S. M. Collins. Boca Raton, FL: CRC, 1991, p. 95-108.
25. Percy, W. H., and J. Christensen. Antibiotic depression of evoked and spontaneous responses of opossum distal colonic muscularis mucosae in vitro: a factor in antibiotic-associated colitis? Gastroenterology 88: 964-970, 1985.
26. Percy, W. H., and J. Christensen. Pharmacological characterization of opossum distal colonie muscularis mucosae in vitro. Am. J. Physiol. 250 (Gastrointest. Liver Physiol. 13): G98-G102, 1986.
27. Percy, W. H., K. Rose, and M. B. Burton. Pharmacologic characterization of the muscularis mucosae in three regions of the rabbit colon. J. Pharmacol. Exp. Ther. 261: 1136-1142, 1992.
28. Pothoulakis, C., J. T. LaMont, R. E. Eglow, N. G. Gao, J. B. Rubins, T. C. Theoharides, and B. F. Dickey. Characterization of rabbit ileal receptors for Clostridium difficile toxin A: evidence for a receptor-coupled G protein. J. Clin. Invest. 88: 119-125, 1991.
29. Pothoulakis, C., R. Sullivan, D. A. Melnick, G. Triadafilopoulos, A.-S. Gadenne, T. Mesulam, and J. T. Lamont. Clostridium difficile toxin A stimulates intracellular calcium release and chemotactic response in human granulocytes. J. Clin. Invest. 81: 1741-1745, 1988.
30. Riegler, M., R. Sedivy, C. Pothoulakis, G. Hamilton, J. Zacheri, G. Bischof, E. Cosentini, W. Feil, R. Schiessel, J. T. LaMont, and E. Wenzl. Clostridium difficile toxin B is more potent than toxin A in damaging human colonic epithelium in vitro. J. Clin. Invest. 95: 2004-2011, 1995.
31. Sinar, D. R., L. G. Charles, and T. W. Burns. Migrating action potential complex activity in absence of fluid production is produced by B subunit of cholera enterotoxin. Am. J. Physiol. 242 (Gastrointest. Liver Physiol. 5): G47-G51, 1982.
32. Sullivan, N. M., S. Pellet, and T. D. Wilkins. Purification and characterization of toxin A and B from Clostridium difficile. Infect. Immun. 35: 1032-1040, 1982.
33. Vantrappen, G., J. Janssens, J. Hellemans, and Y. Ghoos. The interdigestive motor complex of normal subjects and patients with bacterial overgrowth of the small intestine. J. Clin. Invest. 59: 1158-1166, 1977.
34. Viscidi, R., B. C. Laughton, R. Yolkenm, P. Bo-Linn, T. Moengh, R. W. Ryder, and J. G. Bartlett. Serum antibody response to toxins A and B of Clostridium difficile. J. Infect. Dis. 148: 93-100, 1983.
35. 1-4GlcNAc as a functional receptor. Microecol. Ther. 19: 225-227, 1989.