Fungal protoplasts: Relics or modern objects of molecular research?

Microbiological Research

Volumn 153, Issue 2, 1998, Pages 97-104

  Wostemeyer A ^a   Wostemeyer J ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Cell wall; Fungal protoplasts; Fusion; Karyotype; Transformation

Indexed keywords

CELL SURFACE; CHROMOSOME ISOLATION; FUNGAL GENETICS; GENETIC MANIPULATION; GENETIC RECOMBINATION; ISOLATION PROCEDURE; MICROBIAL ACTIVITY; PROTOPLAST;

EID: 0031655388     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/s0944-5013(98)80026-8     Document Type: Article

References (99)

1. Archer, D. B. (1977): Chitin biosynthesis in protoplasts and subcellular fractions of Aspergillus fumigatus. Biochem. J. 164, 653-658.
2. Arnau, J., Housego, A. P., Oliver, R. P. (1994): The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum. Curr. Genet. 25, 438-444.
3. Arnau, J., Jepsen, L. P., Stroman, P. (1991): Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides. Mol. Gen. Genet. 225, 193-198.
4. Barreto, C. C., Alves, L. C., Aragao, F. J. L., Rech, E., Vainstein, M. H. (1997): High frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus. FEMS Microbiol. Lett. 156, 95-99.
5. Becher, D., Wedler, H., Schulze, H., Bode, R., Kasüske, A., Samsonova, I. (1991): Correlation of biochemical blocks and genetic lesions in leucine auxotrophic strains of the imperfect yeast Candida maltosa. Mol. Gen. Genet. 227, 361-368.
6. Belcour, L. (1975): Cytoplasmic mutations isolated from protoplasts of Podospora anserina. Genet. Res. 25, 155-161.
7. Benito, E. P., Campuzano, V., Lopez-Matas, M. A., De Vicente, J.I., Eslava, A.P. (1995): Isolation, characterization and transformation by autonomous replication of Mucor circinelloides OMPdecase deficient mutants. Mol. Gen. Genet. 248, 126-135.
8. Benito, E. P., Diaz-Minguez, J. M., Itturiaga, E. A., Campuzano, V., Eslava, A. P. (1992): Cloning and sequence analysis of the Mucor circinelloides pyrG gene encoding orotidine-5'-monophosphate decarboxylase: use of pyrG for homologous transformation. Gene 116, 59-67.
9. Birren, B. W., Lai, E., Clark, S. M., Hood, L., Simon, M. I. (1988): Optimized conditions for pulsed field gel electrophoretic separations of DNA. Nucl. Ac. Res. 16, 7563-7582.
10. Burmester, A., Wöstemeyer, J. (1994): Variability in genome organization in the zygomycete Parasitella parasitica. Curr. Genet. 26, 456-460.
11. Burmester, A., Wöstemeyer, A., Wöstemeyer, J. (1990): Integrative transformation of a zygomycete, Absidia glauca, with vectors containing repetitive DNA. Curr. Genet. 17, 155-161.
12. 2+ mobilization pathways at the vacuolar membrane of Candida albicans. J. Biol Chem. 270, 7272-7280.
13. Cardinali, G., Pellegrini, L., Martini, A. (1995): Improvement of chromosomal DNA extraction from different yeast species by analysis of single preparation steps. Yeast 11, 1027-1029.
14. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., Prasker, D. C. (1994): Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
15. Clement, D. J., Stanley, M. S., Attwell, N. A., Clipson, N. J. W., Fincham, D. A., Hooley, P. (1996): Evidence for sltA1 as a salt-sensitive allele of the arginase gene (agaA) in the ascomycete Aspergillus nidulans. Curr. Genet. 29, 462-467.
16. De Vries, O. M., Wessels, J. G. (1975): Chemical analysis of cell wall regeneration and reversion of protoplasts from Schizophyllum commune. Arch. Microbiol. 102, 209-218.
17. Dickman, M.B. (1988): Whole cell transformation of the alfalfa fungal pathogen Colletotrichum trifolii. Curr Genet. 14, 241-246.
18. Durand, N., Reymond, P., Fevre, M. (1993): Randomly amplified polymorphic DNAs assess recombination following an induced parasexual cycle in Penicillium roqueforti. Curr. Genet. 24, 417-420.
19. Elorza, M. V., Rico, H., Gozalbo, D., Sentandreu, R. (1983): Cell wall composition and protoplast regeneration in Candida albicans. Antonie van Leeuwenhoek 49, 457-469.
20. Elorza, M. V., Marcilla, A., Sanjuan, R., Mormeneo, S., Sentandreu, R. (1994): Incorporation of specific wall proteins during yeast and mycelial protoplast regeneration in Candida albicans. Arch. Microbiol. 161, 145-151.
21. Errampalli, D., Kohn, L. M. (1996): Electrophoretic karyotype of Sclerotinia sclerotiorum. Appl. Environ. Microbiol. 62, 4247-4251.
22. Ersek, T., English, J. T., Schoelz, J. E. (1995): Creation of species hybrids of Phytophthora with modified host ranges by zoospore fusion. Phytopathol. 85, 1343-1347.
23. Ferenczy, L. (1981): Microbial protoplast fusion. Symposia of the Soc. for Gen. Microbiol. 31, 1-34.
24. Ferenczy, L., Maraz, A. (1977): Transfer of mitochondria by protoplast fusion in Saccharomyces cerevisiae. Nature 268, 524-525.
25. Ferenczy, L., Kevei, F., Zsolt, J. (1974): Fusion of fungal protoplasts. Nature 248, 793-794.
26. Fincham, J. R. S. (1989): Transformation in fungi. Microbiol. Rev. 53, 148-170.
27. Fischer, M., Durand, R., Fevre, M. (1995): Characterization of the "promoter region" of the enolase-encoding gene enol from the anaerobic fungus Neocallimastix frontalis. sequence and promoter analysis. Curr. Genet. 28, 80-86.
28. Fukuda, M., Harada, Y., Imahori, S., Fukumasa-Nakai, Y., Hayashi, Y. (1995): Inheritance of mitochondrial DNA in sexual crosses and protoplast cell fusions in Lentinula edodes. Curr. Genet. 27, 550-554.
29. Gil., M. L., Casanova, M., Martinez, J. P. (1994): Changes in the cell wall glycoprotein composition of Candida albicans associated to the inhibition of germ tube formation by EDTA. Arch. Microbiol. 161, 489-494.
30. Gonzales-Hernandez, G. A., Herrera-Estrella, L., Rocha-Ramirez, V., Roncero, M. I. G., Gutierrez-Corona, J. F. (1997): Biolistic transformation of Mucor circinelloides. Mycol. Res. 101, 953-956.
31. Groves, D. P., Oliver, S. G. (1984): Formation of intergeneric hybrids of yeast by protoplast fusion of Yarrowia and Klyveromyces species. Curr. Genet. 8, 49-55.
32. Gruber, F., Visser, J., Kubicek, C. P., de Graaff, L. H. (1990): Cloning of the Trichoderma reesei pyrG gene and its use as a homologous marker for a high frequency transformation system. Curr. Genet. 18, 447-451.
33. Gupthar, A. S., Garnett, H. M. (1987): Hybridization of Pichia stipitis with its presumptive imperfect partner Candida shehatae. Curr. Genet. 12, 199-204.
34. Hayes, C. K., Harman, G. E., Woo, S. L., Gullino, M. L., Lorito, M. (1993): Methods for electrophoretic karyotyping of filamentous fungi in the genus Trichoderma. Anal. Biochem. 209, 176-182.
35. Hinnen, A., Hicks, J. B., Fink, G. R. (1978): Transformation of yeast. Proc. Natl. Acad. Sci. USA 75, 1929-1933.
36. Housset, P., Nagy, M., Schwencke, J. (1975): Protoplasts of Schizosaccharomyces pombe. an improved method for their preparation and the study of their guanine uptake. J. Gen. Microbiol. 90, 260-264.
37. Itoh, H., Fukuda, Y., Murata, K., Kimura, A. (1983): Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168.
38. Jeanjean, R., Bedu, S., Rocca-Serra, J., Foucault, C. (1984): Phosphate uptake in the yeast Candida tropicalis: purification of phosphate-binding protein and investigations about its role in phosphate uptake. Arch. Microbiol. 137, 215-219.
39. Jefferson, R. A., Burgess, S. M., Hirsch, D. (1986): ß-glucuronidase from Escherichia coli as a gene-fusion marker. Proc. Natl. Acad. Sci. USA 83, 8447-8451.
40. Johnston, S. A., Anziano, P. Q., Shark, K., Sanford, J. C., Burow, R.A. (1988): Mitochondrial transformation in yeast by bombardment with microprojectiles. Science 240, 1538-1541.
41. Johri, B. N., Alurralde, J. D., Klein, J. (1990): Lipase production by free and immobilized protoplasts of Sporotrichum (Chrysosporium) thermophile Apinis. Appl. Microbiol. Biotechnol. 33, 367-371.
42. Kawamoto, S., Sasaki, T., Itahashi, S., Hatsuyama, Y., Ohno, T. (1993): A mutant allele skt5 affecting protoplast regeneration and killer toxin resistance has double mutations in its wild-type structural gene in Saccharomyces cerevisiae. Biosci. Biotech. Biochem. 57, 1391-1393.
43. Kayser, T., Wöstemeyer, J. (1991): Electrophoretic karyotype of the zygomycete Absidia glauca: evidence for differences between mating types. Curr. Genet. 19, 279-284.
44. Keller, U. (1983): Highly efficient mutagenesis of Claviceps purpurea by using protoplasts. Appl. Environ. Microbiol. 46, 580-584.
45. Kevei, F., Toth, B., Coenen, A., Hamari, Z., Varga, J., Croft, J. H. (1997): Recombination of mitochondrial DNAs following transmission of mitochondria among incompatible strains of black Aspergilli. Mol. Gen. Genet. 254, 379-388.
46. Kitamoto, K., Kimura, K., Gomi, K., Kumagai, C. (1994): Electrophoretic karyotype and gene assignment to chromosomes of Aspergillus oryzae. Biosci. Biotech. Biochem. 58, 1467-1470.
47. Klein, T. M., Fromm, M., Weissinger, A., Tomes, D., Schaaf, S., Sletten, M., Sanford, J. C. (1988): Transfer of foreign genes into intact maize cells with high-velocity microprojectiles. Proc. Natl. Acad. Sci. USA 85, 4305-4309.
48. Kobori, H., Toda, T., Yaguchi, H., Toya, M., Yanagida, M., Osumi, M. (1994): Fission yeast protein kinase C gene homologues are required for protoplast regeneration: a functional link between cell wall formation and cell shape. J. Cell Sci. 107, 1131-1136.
49. Kopecka, M., Gabriel, M. (1995): Actin cortical cytoskeleton and cell wall synthesis in regenerating protoplasts of the Saccharomyces cerevisiae actin mutant DBY 1693. Microbiol. 141, 1289-1299.
50. Kottutz, E., Rapp, P. (1990): 1,3-ß-glucan synthase in cell-free extracts from mycelium and protoplasts of Sclerotium glucanicum. J. Gen. Microbiol. 136, 1517-1523.
51. Le Chevanton, L., Leblon, G., Lebilcot, S. (1989): Duplications created by transformation in Sordaria macrospora are not inactivated during meiosis. Mol. Gen. Genet. 218, 390-396.
52. Lew, R. R., Garrill, A., Covic, L., Heath, I. B., Serlin, B. S. (1992): Novel ion channels in the protists, Mougeotia and Saprolegnia, using sub-gigaseals. FEBS Lett. 310, 219-222.
53. Marcilla, A., Mormeneo, S., Elorza, M. V., Manclus, J. J., Sentandreu, R. (1993): Wall formation by Candida albicans yeast cells: synthesis, secretion and incorporation of two types of mannoproteins. J. Gen. Microbiol. 139, 2985-2993.
54. Mc Cluskey, K., Russell, B.W., Mills, D. (1990): Electrophoretic karyotyping without the need for generating protoplasts. Curr. Genet. 18, 385-386.
55. Mönke, E., Schäfer, W. (1993): Transient and stable expression in the fungal maize pathogen Cochliobolus heterostrophus after transformation with the beta-glucuronidase (GUS) gene. Mol. Gen. Genet. 241, 73-80.
56. Morgan, A. J. (1983): Yeast strain improvement by protoplast fusion and transformation. Experientia Suppl. 46, 155-166.
57. Motoyama, T., Sudoh, M., Horiuchi, H., Ohta, A., Takagi, M. (1994): Isolation and characterization of two chitin synthase genes of Rhizopus oligosporus. Biosci. Biotech. Biochem. 58, 1685-1693.
58. Murakami, K., Aikawa, J., Horinouchi, S., Beppu, T. (1993): Characterization of an aspartic proteinase of Mucor pusillus expressed in Aspergillus oryzae. Mol. Gen. Genet. 241, 312-318.
59. Nagy, A., Vagvölgyi, C. S., Balla, E., Ferenczy, L. (1994): Electrophoretic karyotype of Mucor circinelloides. Curr. Genet. 26, 45-48.
60. Nara, F., Watanabe, I., Serizawa, N. (1993); Cloning and sequencing of the 3-phosphoglycerate kinase (PGK) gene from Penicillium citrinum and its application to heterologous gene expression. Curr. Genet. 23, 134-140.
61. Noel, T., Labarere, J. (1994): Homologous transformation of the edible basidiomycete Agrocybe aegerita with the URA1 gene: characterization of integrative events and of rearranged free plasmids in transformants. Curr. Genet. 25, 432-437.
62. Oh, K., Matsuoka, H.,Teraoka, T., Sumita, O., Takatori, K., Kurata, H. (1993): Effects of antimycotics on the biosynthesis of cellular macromolecules in Aspergillus niger protoplasts. Mycopathologia 122, 135-141.
63. Ohgi, K., Shiratori, Y., Nakajima, A., Iwama, M., Kobayashi, H., Inokuchi, N., Koyama, T., Köck, M., Löffler, A., Glund, K., Irie, M. (1997): The base specificities of tomato ribonuclease (RNase LE) and its asp-44 mutant enzyme expressed from yeast cells. Biosci. Biotech. Biochem. 61, 431-438.
64. Osiewac, H.D., Ridder, R. (1991): Genome analysis of imperfect fungi: electrophoretic karyotyping and characterization of the nuclear gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) of Curvularia lunata. Curr. Genet. 20, 151-155
65. Ozeki, K., Kyoya, F., Hizume, K., Kanda, A., Hamachi, M., Nunokawa, Y. (1994): Transformation of intact Aspergillus niger by electroporation. Biosci. Biotech. Biochem. 58, 2224-2227.
66. Parker, D. M., Hilber, U. W., Bodmer, M., Smith, F. D., Yao, C., Köller, W. (1995): Production and transformation of conidia of Venturia inaequalis. Phytopathol. 85, 87-91.
67. Peberdy, J. F. (1987): Developments in protoplast fusion in fungi. Microbiol Sci. 4, 108-114.
68. Pendland, J. C., Hung, S.-Y., Boucias, D. G. (1993): Evasion of host defense by in vivo-produced protoplast-like cells of the insect mycopathogen Beauveria bassiana. J. Bacteriol. 175, 5962-5969.
69. Perkins, D. D., Kinsey, J. A., Asch, D. K., Frederick, G. D. (1993): Chromosome rearrangements recovered following transformation of Neurospora crassa. Genetics 134, 729-736.
70. Pieterse, C. M., van't Klooster, J., van den Berg-Velthuis, G. C., Covers, F. (1995): NiaA, the structural nitrate reductase gene of Phytophthora infestans: isolation, characterization and expression analysis in Aspergillus nidulans. Curr. Genet. 27, 359-366.
71. Pitt, D., Barnes, J. C. (1993): Calcium homeostasis, signalling and protein phosphorylation during calcium-induced conidiation in Penicillium notatum. J. Gen. Microbiol. 139, 3053-3063.
72. Plummer, K. M., Howlett, B. J. (1995): Inheritance of chromosomal length polymorphisms in the ascomycete Leptosphaeria maculons. Mol Gen. Genet. 247, 416-422.
73. Provost, A., Bourguignon, C., Fournier, P., Ribet, A. M., Heslot, H. (1978): Intergeneric hybridization in yeasts through protoplast fusion. FEMS Microbiol Lett. 3, 309-312.
74. Revuelta, J. L., Jayaram, M. (1986): Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid. Proc. Natl. Acad. Sci. USA 83, 7344-7347.
75. Sarachek, A., Lovchik, J. A. (1989): Unidirectional internuclear transfer of linked genes in heterokaryons of Candida albicans. J. Basic Microbiol. 29, 527-535.
76. Sietsma, J. H., Beth Din, A., Ziv, V., Sjollema, K. A., Yarden, O. (1996): The localization of chitin synthase in membraneous vesicles (chitosomes) in Neurospora crassa. Microbiol. 142, 1591-1596.
77. Skory, C. D., Horng, J. S., Pestka, J. J., Linz, J. E. (1990): Transformation of Aspergillus parasiticus with a homologous gene (pyrG) involved in pyrimidine biosynthesis. Appl. Environ. Microbiol. 56, 3315-3320.
78. Sone, T., Abe, T., Suto, M., Tomita, F. (1997): Identification and characterization of a karyotypic mutation in Magnaporthe grisea. Biosci. Biotech. Biochem. 61, 81-86.
79. Streatfield, S. J., Roberts, C. F. (1993): Disruption of the 3'phosphoglycerate kinase (pgk) gene in Aspergillus nidulans. Curr. Genet. 23, 123-128.
80. Stunnenberg, H. G., van den Broek, H. W. J. (1982): Effect of alpha-amanitin and actinomycin D on RNA synthesis in the protoplasts of Aspergillus nidulans. Microbios. 35, 197-207.
81. Takaya, N., Yanai, K., Horiuchi, H., Ohta, A., Takagi, M. (1994): Cloning and characterization of two 3-phosphoglycerate kinase genes of Rhizopus niveus and heterologous gene expression using their promoters. Curr. Genet. 25, 524-530.
82. Taylor, J. L., Borgmann, I., Seguin-Swartz, G. (1991): Electrophoretic karyotyping of Leptosphaeria maculans differentiates highly virulent from weakly virulent isolates. Curr. Genet. 19, 273-277.
83. Timberlake, W. E., Marshall, M. A. (1989): Genetic engineering of filamentous fungi. Science 244, 1313-1317.
84. Torrez-Guzman, J. C., Arreola-Garcia, G. A., Zazueta-Sandoval, R., Carrillo-Rayas, T., Martinez-Cadena, G., Gutierrez-Corona, F. (1994): Genetic evidence for independence between fermentative metabolism (ethanol accumulation) and yeast-cell development in the dimorphic fungus Mucor rouxii. Curr. Genet. 26, 166-171.
85. Tsuchiya, K., Nagashima, T., Yamamoto, Y., Gomi, K., Kitamoto, K., Kumagai, C., Tamura, G. (1994): High level secretion of calf chymosin using a glucoamylase-prochymosin fusion gene in Aspergillus oryzae. Biosci. Biotech. Biochem. 58, 895-899.
86. Ugalde, U. O., Virto, M. D., Pitt, D. (1990): Calcium binding and induction of conidiation in protoplasts of Penicillium cyclopium. Antonie van Leeuwenhoek 57, 43-49.
87. Ugalde, U. O., Hernandez, A., Galindo, I., Pitt, D., Barnes, J. C., Wakley, G. (1992): Preparation of right-side-out plasma membrane vesicles from Penicillium cyclopium: a critical assessment of markers. J. Gen. Microbiol. 138, 2205-2212.
88. Vagvölgyi, C., Ferenczy, L. (1991): Isolation of nuclei from Aspergillus nidulans protoplasts. FEMS Microbiol Lett. 82, 247-251.
89. Van Heeswijck, R., Roncero, M. I. G. (1984): High frequency transformation of Mucor with recombinant plasmid DNA. Carlsberg Res. Commun. 49, 691-702.
90. Van Zyl, W.-H., Lodolo, E. J., Gericke, M. (1993): Conversion of homothallic yeast to heterothallism through HO gene disruption. Curr. Genet. 23, 290-294.
91. Villa, T. G., Notario, V., Benitez, T., Villanueva, J. R. (1975): Synthesis of ß-glucanase and other extracellular proteins by cells and protoplasts of the yeast Pichia polymorpha. Arch. Microbiol. 105, 335-337.
92. Wada, M., Beppu, T., Horinouchi, S. (1996): Integrative transformation of the zygomycete Rhizomucor pusillus by homologous recombination. Appl. Microbiol. Biotechnol. 45, 652-657.
93. Watanabe, M., Gomi, S., Tohyama, H., Ohtsuka, K., Shibahara, S., Inouye, S., Kobayashi, H., Suzuki, S., Kondo, S., Takeuchi, T., Yamaguchi, H. (1996): Binding of benanomicin A to fungal cells in reference to its fungicidal action J. Antibiot. 49, 366-373.
94. Weber, G., Leitner, E. (1994): Disruption of the cyclosporin synthetase gene of Tolypocladium niveum. Curr. Genet. 26, 461-467.
95. Wemars, K., Goosen, T., Wennekes, L. M., Visser, J., Bos, C. J., van den Broek, H. W., van Gorcom, R. F., van den Hondel, C. A., Pouwels, P. H. (1985): Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene. Curr. Genet. 9, 361-368.
96. Wilmanska, D., Milczarek, K., Rumijowska, A., Bartnicka, K., Sedlaczek, L. (1992): Elimination of by-products in 11 beta-hydroxylation of substance S using Curvularia lunata clones regenerated from NTG-treated protoplasts. Appl. Microbiol. Biotechnol. 37, 626-630.
97. Wöstemeyer, J., Burmester, A., Weigel, C. (1987): Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca. Curr. Genet. 12, 625-627.
98. Xuei, X., Skatrud, P. L. (1994): Molecular karyotype alterations induced by transformation in Aspergillus nidulans are mitotically stable. Curr. Genet. 26, 225-227.
99. Zhou, X.-L., Stumpf, M. A., Hoch, H. C., Kung, C. (1991): A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces. Science 253, 1415-1417.