Characterization of CHO-K1 Cells Stably Expressing PDE-IV Enzymes: Whole-Cell cAMP Determinations vs Broken-Cell Enzymatic Assays

Cell Biochemistry and Biophysics

Volumn 29, Issue 1-2, 1998, Pages 159-178

  Pon, D J ^a   Plant, M ^a   Tkach, J ^a   Boulet, L ^a   Muise, E ^a   Allen, R A ^b   Rodger, I W ^a  

^a MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

^b UCB PHARMA   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

3',5' CYCLIC NUCLEOTIDE PHOSPHODIESTERASE; CYCLIC AMP; PHOSPHODIESTERASE INHIBITOR; PHOSPHODIESTERASE IV; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CATALYSIS; CHEMISTRY; CHO CELL; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ACTIVATION; ENZYMOLOGY; EOSINOPHIL; GENETIC TRANSFECTION; GENETICS; GUINEA PIG; HAMSTER; HUMAN; HYDROLYSIS; METABOLISM;

3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE; ANIMALS; CATALYSIS; CHO CELLS; CRICETINAE; CYCLIC AMP; ENZYME ACTIVATION; EOSINOPHILS; GUINEA PIGS; HUMANS; HYDROLYSIS; PHOSPHODIESTERASE INHIBITORS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 0031626726     PISSN: 10859195     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02737834     Document Type: Article

References (16)

1. Schudt, C., Tenor, H., and Hatzelmann, A. (1995) PDE isoenzymes as targets for anti-asthma drugs. Eur. Respir. J. 8, 1179-1183.
2. Ortiz, J. L., Valles, J. M., Marti-Cabrera, M., Cortijo, J., and Morcillo, E. J. (1996) Effects of selective phosphodiesterase inhibitors on platelet-activating factor- and antigen-induced airway hyperreactivity, eosinophil accumulation, and microvascular leakage in guinea pigs. Arch. Pharmacol. 353, 200-206.
3. Lagente, V., Moodley, I., Perrin, S., Mottin, G., and Junien, J. L. (1994) Effects of isozyme-selective phosphodiesterase inhibitors on eosinophil infiltration in the guinea-pig lung. Eur. J. Pharmacol. 255, 253-256.
4. Boie, Y., Rushmore, T. H., Darmongoodwin, A., Grigorczyk, R., Slipetz, D. M., Metters, K. M., and Abramovitz, M. (1994) Cloning and expression of a cDNA for the human prostanoid IP receptor. J. Biol Chem. 269, 12,173-12,178.
5. Kroegel, C., Giembycz, M. A., and Barnes, P. J. (1990) Characterization of eosinophil cell activation by peptides. J. Immunol. 145, 2581-2587.
6. Perry, M. J., O'Connell, J., Walker, C., Crabbe, T., Baldock, D., Russell, et al. CDP 840, a novel inhibitor of phosphodiesterase Type 4 (submitted).
7. Cheng, H. C., Kemp, B. E., Pearson, R. B., Smith, A. J., Misconi, L., Van Patten, S. M., and Walsh, D. A. (1986) A Potent synthetic peptide inhibitor of the cAMP-dependent protein kinase. J. Biol. Chem. 261, 989-992.
8. Schneider, H. H., Schmlechen, R., Brezinski, M., and Seidler, J. (1986) Stereospecific binding of the anti-depressant rolipram to brain protein structures. Eur. J. Pharm. 127, 105-115.
9. Corbin, J. D., Sugden, P. H., Lincoln, T. M., and Keely, S. L. (1977) Compartmentalization of Adenosine 3′5′-monophosphate and adenosine 3′5′-monophosphate-dependent protein kinase in heart tissue. J. Biol. Chem. 11, 3854-3861.
10. Barnes, P. J. (1995) Cyclic nucleotides and phosphodiesterases and airway function. Eur. Respir. J. 8, 457-462.
11. Robinson, G. A., Butcher, R. W., and Sutherland, E. W. (1971) Cyclic AMP. Academic, New York.
12. Souness, J. E., Maslen, C., Webber, S., Foster, M., Raeburn, D., Palfreyman, M. N., Ashton, M. J., and Karlsson, J. A. (1995) Suppression of eosinophil function by RP-73401, a potent and selective inhibitor of cyclic AMP-specific phosphodiesterase - comparison with rolipram. Br. J. Pharmacol. 115, 39-46.
13. Alvarez, R., Sette, C., Yang, D., Eglen, R. M., Wilhelm, R., Shelton, E. R., and Conti, M. (1995) Activation and selective inhibition of a cyclic AMP-specific phosphodiesterase, PDE-4D3. Mol. Pharm. 48, 616-622.
14. Barnette, M. S., Grous, M., Cieslinski, L. B., Burman, M., Christensen, S. B., and Torphy, T. J. (1995) Inhibitors of phosphodiesterase IV (PDE IV) increase acid secretion in rabbit isolated gastric glands: xorrelationb etween function and interaction with a high-affinity rolipram binding site. J. Pharmacol. Exper. Ther. 273, 1396-1402.
15. Schneider, H. H., Yamaguchi, M., Andrews, J. S., and Stephens, D. N. (1995) Discriminative stimulus properties of the steroisomers of the phosphdiesterase inhibitor rolipram. Pharmacol. Biochem. Behav. 50, 211-217.
16. Souness, J. E., Griffin, M., Maslen, C., Ebsworth, K., Scott, L. C., Pollock, K., et al. (1996) Evidence that cyclic AMP phosphodiesterase inhibitors suppress TNFα generation from human monocytes by interacting with a "low-affinity" phosphodiesterase 4 conformer. Br. J. Pharmacol. 118, 649-658.