A new design for large, dilute sample loading in capillary electrophoresis

Journal of Capillary Electrophoresis and Microchip Technology

Volumn 5, Issue 1-2, 1998, Pages 1-7

  Begona Barroso, M ^a   De Jong, Ad P ^b  

^a UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

^b NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

Author keywords

Capillary electrophoresis; In line preconcentration; Large volume injection; Peptides; Sample fractionation; Transient isotachophoresis; Trapping column

Indexed keywords

ELECTROLYTE; ORGANIC SOLVENT; PEPTIDE;

ARTICLE; CAPILLARY ZONE ELECTROPHORESIS; CONCENTRATION (PARAMETERS); DIAGNOSTIC VALUE; ELUTION; FLOW KINETICS; FRACTIONATION; HYDRODYNAMICS; ISOTACHOPHORESIS; MAJOR HISTOCOMPATIBILITY COMPLEX; MASS SPECTROMETRY; NANOPARTICLE; PROTEIN ANALYSIS; SEPARATION TECHNIQUE; AMINO ACID SEQUENCE; CAPILLARY ELECTROPHORESIS; METHODOLOGY; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; ELECTROPHORESIS, CAPILLARY; MOLECULAR SEQUENCE DATA; PEPTIDES;

EID: 0031598233     PISSN: 10795383     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Castagnola M, Cassiano L, Messana I, Nocca G, Rabino R, Rosseti DV, Giardina B. Capillary zone electrophoresis of peptides: prediction of the electrophoretic mobility and resolution. J Chromatogr B: Biomed Appl 1994; 656:87-97.
2. Cifuentes A, Poppe H. Simulation and optimization of peptide separation by capillary electrophoresis. J Chromatogr A 1994; 680:321-40.
3. Oda RP, Madden BJ, Morris JC, Spelsberg TC, Landers JP. Multiple-buffer-additive strategies for enhanced capillary electrophoretic separation of peptides. J Chromatogr A 1994; 680:341-51.
4. Righetti PG, Gelfi C. Capillary electrophoresis of DNA for molecular diagnostics. Electrophoresis 1997; 18:1709-14.
5. Righetti PG, Gelfi C. Recent advances in capillary electrophoresis of DNA fragments and PCR products. Biochem Soc Trans 1997; 25:267-73.
6. Ng CL, Ong CP, Lee HK, Li SFY. Determination of pharmaceuticals and related impurities by capillary electrophoresis. J Chromatogr A 1994; 680:579-86.
7. Holland LA, Chetwyn NP, Perkins MD, Lunte SM. Capillary electrophoresis in pharmaceutical analysis. Pharm Res 1997; 14:372-87.
8. Frokiaer H, Moller P, Sorensen H, Sorensen S. High-performance capillary electrophoresis for characterization of hapten-protein conjugates used for production of antibodies against soyasaponin I. J Chromatogr 1994; 680:437-45.
9. Reif OW, Freitag R. Control of the cultivation process of antithrombin III and its characterization by capillary electrophoresis. J Chromatogr 1994; 680:383-94.
10. Wynia GS, Windhorst G, Post PC, Maris FA. Development and validation of a capillary electrophoresis method within a pharmaceutical control environment and comparison with high-performance liquid chromatography. J Chromatogr A 1997; 773:339-50.
11. Cheicante RL. Capillary electrophoretic techniques used in the 1st provisional secretariat interlaboratory comparison test. In: Berg DA, ed. Proceedings of the 1996 ERDEC Scientific Conference on Chemical and Biological Research, 19-22 Nov 1996. Performr: Edgewood Research Development and Engineering Center, Aberdeen Proving Ground, MD: National Technical Information Service, 1996:711-7.
12. Capillary electrophoresis: theory and practice. In: Grossman PD, Colburn JC, eds. New York: Academic Press, 1992.
13. Handbook of capillary electrophoresis. In: Landers JP, ed. Boca Raton, FL: CRC Press, 1997.
14. Hofstadler SA, Swanek FD, Gale DC, Ewing AG, Smith RD. Capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for direct analysis of cellular proteins. Anal Chem 1995; 67:1477-80.
15. Kurian E, Prendergast FG, Tomlinson AJ, Holmes MW, Naylor S. Identifying sites of protein modification by using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and on-line membrane preconcentration-capillary electrophoresis-tandem mass spectrometry. J Am Soc Mass Spectrom 1997; 8:8-14.
16. Shimura K, Karger BL. Affinity probe capillary electrophoresis: analysis of recombinant human growth hormone with a fluorescent labeled antibody fragment. Anal Chem 1994; 66:9-15.
17. Tomlinson AJ, Guzman NA, Naylor S. Enhancement of concentration limits of detection in CE and CE-MS: a review of on-line sample extraction, cleanup, analyte preconcentration, and microreactor technology. J Cap Elec 1995; 2(6):247-66.
18. Burgi DS, Chien RL. Optimization in sample stacking for high-performance capillary electrophoresis. Anal Chem 1991; 63:2042-7.
19. Burgi DS, Chien RL. Sample stacking of an extremely large injection volume in high-performance capillary electrophoresis. Anal Chem 1992; 64:1046-50.
20. Shihabi ZK. Peptide stacking by acetonitrile-salt mixtures for capillary zone electrophoresis. J Chromatogr A 1996; 744:231-40.
21. Mazzeo JR, Martineau JA, Krull IS. Peptide mapping using EOF-driven capillary isoelectric focusing. Anal Biochem 1993; 208:323-9.
22. Clarke NJ, Tomlinson AJ. On-line desalting of physiologically derived fluids in conjunction with capillary isoelectric focusing-mass spectrometry. J Am Soc Mass Spectrom 1997; 8:743-8.
23. Bocek P, Gebauer P, Dolnik V, Foret F. Recent developments in isotachophoresis. J Chromatogr 1985; 334:157-95.
24. Foret F, Szoko E, Karger BL. On-column transient and coupled column isotachophoretic preconcentration of protein samples in capillary zone electrophoresis. J Chromatogr 1992; 608:3-12.
25. Kasicka V, Prusik Z, Smekal O, Hlavacek J, Barth T, Weber G, Wagner H. Application of capillary and free-flow zone electrophoresis and isotachophoresis to the analysis and preparation of the synthetic tetrapeptide fragment of growth hormone-releasing peptide. J Chromatogr B: Biomed Appl 1994; 656:99-106.
26. Foret F, Szoko E, Karger BL. Trace analysis of proteins by CZE with on-column transient isotachophoretic preconcentration. Electrophoresis 1997; 14:417-28.
27. Bergmann J, Jaehde U, Mazereeuw M, Tjaden UR, Schunack W. Potential of on-line isotachophoresis-capillary zone electrophoresis with hydrodynamic counterflow in the analysis of various basic proteins and recombinant human interleukin-3. J Chromatogr A 1996; 734:381-9.
28. Chien RL, Burgi DS. Field amplified sample injection in high-performance capillary electrophoresis. J Chromatogr 1991; 559:141-52.
29. Chien RL, Burgi DS. Field-amplified polarity switching sample injection in high-performance capillary electrophoresis. J Chromatogr 1991; 559:153-61.
30. Chien RL, Burgi DS. On-line sample concentration using field amplification in CZE. Anal Chem 1992; 64:489A-96A.
31. Huang X, Zare RN. Continuous sample collection in capillary zone electrophoresis by coupling the outlet of a capillary to a moving surface. J Chromatogr 1990; 516:185-9.
32. Guzman NA, Trebilcock MA, Advis JP. The use of a concentration step to collect urinary components separated by capillary electrophoresis and further characterization of collected analytes by mass spectrometry. J Liq Chromatogr 1991; 14:997-1015.
33. Lee HG, Desiderio DM. Preparative capillary zone electrophoresis of synthetic peptides conversion of an autosampler into a fraction collector. J Chromatogr 1994; 686:309-17.
34. Muller O, Foret F, Karger BL. Design of a high-precision fraction collector for capillary electrophoresis. Anal Chem 1995; 67:2974-80.
35. Strausbauch MA, Madden BJ, Wettstein PJ, Landers JP. Sensitivity enhancement and second-dimensional information from solid-phase extraction-capillary electrophoresis of entire high-performance liquid chromatography fractions. Electrophoresis 1995; 16:5418.
36. Beattie JH, Self R, Richards MP. The use of solid-phase concentrators for on-line preconcentration of metallothionein prior to isoform separation by capillary zone electrophoresis. Electrophoresis 1995; 16:3228.
37. Strausbauch MA, Xu SJ, Ferguson JE, Nuñez ME, Machacek D, Lawson GM, Wettstein PJ, Landers JP. Concentration and separation of hypoglycemic drugs using solid-phase extraction-capillary electrophoresis. J Chromatogr A 1995; 717:279-91.
38. Strausbauch MA, Landers JP, Wettstein PJ. Mechanism of peptide separations by solid-phase extraction capillary electrophoresis at low pH. Anal Chem 1996; 68:306-14.
39. Moseley MA, Deterding LJ, Tomer KB, Jorgenson W. Nanoscale packed-capillary liquid chromatography coupled with mass spectrometry using a coaxial continuous-flow fast atom bombardment interface. Anal Chem 1991; 63:1467-73.
40. Swartz ME, Merion M. On-line sample preconcentration on a packed-inlet capillary for improving the sensitivity of capillary electrophoretic analysis of pharmaceuticals. J Chromatogr 1993; 632:209-13.
41. Tomlinson AJ, Benson LM, Braddock WD, Oda RP, Naylor S. On-line preconcentration-capillary electrophoresis-mass spectrometry (PC-CE-MS). J High Res Chromatogr 1994; 17:729-32.
42. Tomlinson AJ, Naylor S. Systematic development of on-line membrane preconcentration capillary electrophoresis-mass spectrometry for the analysis of peptide mixtures. J Cap Elec 1995; 2:225-33.
43. Tomlinson AJ, Naylor S. A strategy for sequencing peptides from dilute mixtures at the low femtomole level using membrane pre-concentration-capillary electrophoresis-tandem mass spectrometry (mPC-CE-MS/MS). J Liq Chromatogr 1995; 18:3591-615.
44. Tomlinson AJ, Benson LM, Braddock WD, Oda RP, Naylor S. Improved on-line membrane preconcentration-capillary electrophoresis (mPC-CE). J High Res Chromatogr 1995; 18:381-3.
45. Tomlinson AJ, Naylor S. Enhanced performance membrane pre-concentration-capillary electrophoresis-mass spectrometry (mPC-CE-MS) in conjunction with transient isotachophoresis for analysis of peptide mixtures. J High Res Chromatogr 1995; 18:384-6.
46. Tomlinson AJ, Benson LM, Oda RP, Naylor S. Novel modifications and applications of preconcentration-capillary electrophoresis-mass spectrometry. J Cap Elec 1995; 2:97-104.
47. Tomlinson AJ, Benson LM, Jameson S, Johnson DH, Naylor S. Utility of membrane preconcentration capillary electrophoresis mass spectrometry in overcoming limited sample loading for analysis of biologically derived drug metabolites, peptides and proteins. J Am Soc Mass Spectrom 1997; 8:15-24.