Characterization of [125I]sauvagine binding to CRH2 receptors: Membrane homogenate and autoradiographic studies

Journal of Pharmacology and Experimental Therapeutics

Volumn 286, Issue 1, 1998, Pages 459-468

  Rominger, David H ^a   Rominger, Cynthia M ^a   Fitzgerald, Lawrence W ^a   Grzanna, Reinhard ^a   Largent, Brian L ^a   Zaczek, Robert ^a  

^a DUPONT COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CORTICOTROPIN RELEASING FACTOR RECEPTOR; GUANYLYLIMIDODIPHOSPHATE; RECOMBINANT CORTICOTROPIN RELEASING FACTOR; SAUVAGINE; UNCLASSIFIED DRUG; UROCORTIN; UROTENSIN; DIAGNOSTIC AGENT; PEPTIDE; RADIOACTIVE IODINE;

ARTICLE; AUTORADIOGRAPHY; BINDING SITE; DOSE TIME EFFECT RELATION; DRUG POTENCY; DRUG RECEPTOR BINDING; EQUILIBRIUM CONSTANT; HORMONE RECEPTOR INTERACTION; HUMAN; HUMAN CELL; INHIBITION KINETICS; MOLECULAR CLONING; PRIORITY JOURNAL; RECEPTOR AFFINITY; TISSUE DISTRIBUTION; ANIMAL; BRAIN; CELL LINE; MALE; METABOLISM; RAT; SPRAGUE DAWLEY RAT; TEMPERATURE;

ANIMALS; AUTORADIOGRAPHY; BRAIN; CELL LINE; GUANYLYL IMIDODIPHOSPHATE; HUMANS; IODINE RADIOISOTOPES; MALE; PEPTIDES; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, CORTICOTROPIN-RELEASING HORMONE; TEMPERATURE;

EID: 0031597628     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Aguilera G, Millan MA, Hauger RL and Catt KJ (1987) Corticotropin-releasing factor receptors: distribution and regulation in brain. pituitary and peripheral tissues, in The Hypothalamic-Pituitary-Adrenal Axis Reuisted, pp 48-66, Annals of the New York Academy of Sciences, New York.
2. Berkenbosch F, Schipper J and Tilders FJ (1986) Corticotropin-releasing factor immunostaining in the rat spinal cord and medulla oblongata: An unexpected form of cross-reactivity with substance P. Brain Res 399:87-96.
3. Bradfor MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principles of protein dye-binding. Anal Biochem 72:248-254.
4. Canteras NS, Simerly RB and Swanson LW (1992) Connections of the posterior nucleus of the amygdala. J Comp Neurol 324:143-179.
5. Chalmers DT, Lovenberg TW and De Souza EB (1995) "Localization of novel corticotropin-releasing factor receptor (CRF2) mRNA to specific sub-cortical nuclei in rat brain: comparison with CRF1 receptor mRNA expression. J Neurosci 15:6340-6350.
6. Danaldson CJ, Sutton SW, Perrin MH, Corrigan AZ, Lewis KA, Rivier JE, Vaughan JM and Vale WW (1996) Cloning and characterization of human urocortin. Endocrinology 137:3896.
7. De Souza EB (1987) Corticotropin-releasing factor receptors in the rat central nervous system: Characterization and regional distribution. J Neurosci 7:88-100.
8. De Souza EB and Grigoriadis DE (1995) Corticotropin-releasing factor: physiology, pharmacology, and role in central nervous system and immune disorders, in Psychopharmacology: The Fourth Generation of Progress, pp 505-517, Raven Press, New York.
9. De Souza EB and Insel TR (1990) Corticotropin-releasing factor (CRF) receptors in the rat nervous system: Autoradiographic localization studies, in CorticotropinReleasing Factor: Basic and Clinical Studies of a Neuropeptid, pp 69-90, CRC Press, Boca Raton, FL.
10. Donner DB, Casade J, Hartstein L, Martin D and Sonenberg M (1980) Characterization of the slowly dissociable human growth hormone binding component of isolated rat hepatocytes. Biochemistry 19:39-46.
11. Duggan N, De Souza EB and Grigoriadis DE (1997) Selective labeling of CRF2 receptors in the rat olfactory bulb using [125I]sauvagine: radiolabeling and autoradiographic studies. Soc Neurosci Abst 23:1765.
12. Dunn AJ and Berridge CW (1990) Physiological and behavioral responses to corticotropin-releasing factor administration: is CRF a mediator of anxiety of stress responses? Brain Res Rev 15:71-100.
13. Fitzgerald LW, Gilligan P, Culp S, Horlick R, Largent B, Aguilera G, Keim S, Krause C, Marshall A, Patterson J, Rominger C, Rominger D, Logue A, Bakthavatchalam R, Arvanitis A, Christos T, Cocuzza A and Zaczek R (1996) Identification of potent selective non-peptide antagonists for the human corticotropin-releasing hormone 1 (CRH1) receptor. Soc Neurosci Abstr 22:1546.
14. Fleming AS, Miceli M, Morreto D (1983) Lesion of the medial preoptic area prevent the facilitation of maternal behavior produced by amydala lesions. Physiol Behav 31:503-510.
15. Graeser D and Neubig RR (1992) Methods for the study of receptor/G-protein interactions, in Signal Transduction, a Practical Approach (Milligan G ed) pp 1-30, IRL Press, New York.
16. 125I-Tyro-sauvagine: A novel high affinity radioligand for the pharmacological and biochemical study of human corticotropin-releasing factor 2 alpha receptors. Mol Pharmacol 50:679-86.
17. Horlick RA, Sperle K, Breth LA, Reid CC, Shen ES, Robbing AK, Cooke GM and Largent BL (1997) Rapid generation of stable cell lines expressing corticotropin-releasing hormone receptor for drug discovery. Protein Expression Purification 9:301-308.
18. Kishimoto TR, Pearse II RV, Lin CR and Rosenfield MG (1995) A sauvagine/ corticotropin-releasing hormone factor receptor expressed in heart and skeletal muscle. Proc Natl Acad Sci USA 92:1108-1112.
19. Kostich W, Chen A, Sperle K, Horlick RA, Patterson J, Largent BL (1996) Molecular cloning and expression analysis of human CRH receptor type 2 alpha and beta isoforms. Soc Neurosci Abstr 22:1545.
20. Ladd CO, Owens MJ and Nemeroff CB (1996) Persistent changes in corticotropin-releasing factor neuronal systems induced by maternal deprivation. Endocrinology 137:1212-8.
21. Liebsch, Landgraf GR, Gertsberger R, Probst JC, Wotjak CT, Englemenn M, Holsboer F and Montkowski A (1995) Chronic infusion of a CRH1 receptor antisense oligodeoxynucleotide into the central nucleus of the amygdala reduced anxiety-related behavior in socially defeated rats. Regul Peptides 59:229-239.
22. Lovenberg TW, Liaw CW, Grigoriadis DE, Clevenger W, Chalmer DT, De Souza EB and Oltersdorf T (1995) "Cloning and characterization of a functionally distinct corticotropin-releasing factor receptor subtype from rat brain [published erratum appears in Proc Natl Acad Sci U S A 1995 Jun 6;92(12):5759]." Proc Natl Acad Sci USA 92:836-40.
23. Lundkvist, Chai JZ, Teheranian R, Hasaven H, Bartfai T, Jenck F, Widmer U and Moreau J-L (1996) A non-peptidic corticotropin releasing factor receptor antagonist attenuates fever and exhibits anxiolytic-like activity. Eur J Pharamcol 309: 195-200.
24. Munson PJ and Rodbard E (1980) A versatile computerized approach for characterization of ligand-binding systems. Anal Biochem 107:220-239.
25. Owens MJ and Nemeroff CB (1991) Physiology and pharmacology of corticotropin-releasing factor. Pharmacol Rev 43:425-473.
26. Perrin MH, Donaldson CJ, Chen R, Lewis KA and Vale WW (1993) Cloning and functional expression of a rat brain corticotropin releasing factor (CRF) receptor. Endocrinology 133:3058-3061.
27. 2 binding sites in adult rat brain. Neuropsychopharmacology 17: 308-316.
28. Raadsheer FC, Oorschot DE, Verwer RW, Tilders FJ and Swaab DF (1994) Age-related increase in the total number of corticotropin-releasing hormone neurons in the human paraventricular nucleus in controls and Alzheimer's disease: comparison of the dissector with an unfolding method. J Compar Neurol 339:447-457.
29. Rominger CM, Zhang W, Ho S, Zaczek R and Fitzgerald LW (1996) Regional distribution of putative CRH2 receptors in rat brain as determined by [125I]sauvagine receptor autoradiography. Soc Neurosci Abst 22:1546.
30. α receptors." Soc Neurosci Abstr 22:1546
31. Sakanaka M, Shibasaki T and Lederis K (1987) Corticotrpin releasing factor-like immunoreactivity in the rat brain as revealed by a cobalt-glucose oxidase-diaminobenzidine method. J Comp Neurol 260:256-298.
32. Schulz DW, Mansbach RS, Sprouse J, Baselton JP, Collins J, Corman M, Dunaikis A, Faraci S, Schmidt AW, Seeger T, Seymour P, Tingley FD., Winston EN, Chen YL and Heym J (1996) CP-154,526: A potent and selective nonpeptide antagonist of corticotropin releasing factor receptors. Proc Natl Acad Sci USA 93:10477-10482.
33. Siggins GR, Gruol D, Aldenhoff J and Pittman Q (1985) Electrophysiological actions of corticotropin-releasing factor in the central nervous system. Fed Proc 44:237-242.
34. Spina M, Merlo-Pich E, Chan RK, Basso AM, Rivier J, Vale W and Koob GF (1996) Appetite-suppressing effects of urocortin, a CRF-related neuropeptide. Science 273:1561-4.
35. Vale W, Spiess J, Rivier C and Rivier J (1981) Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin. Science 213:1394-1397.
36. Vaughan, Donaldson JC, Bittencourt J, Perrin MH, Lewis K, Sutton S, Chan R, Turnbull AV, Lovejoy D, Rivier C, Rivier J, Sawchenko PE and Vale W (1995) Urocortin, a mammalian neuropeptide related to fish urotensin 1 and to corticotropin-releasing factor. Nature 378:287-292.