Mutations at lipid-expossed residues of the acetylcholine receptor affect its gating kinetics

Molecular Pharmacology

Volumn 54, Issue 1, 1998, Pages 146-153

  Bouzat, Cecilia ^a,b   Roccamo, Ana M ^a   Garbus, Ingrid ^a   Barrantes, F J ^a  

^a UNIVERSIDAD NACIONAL DEL SUR   (Argentina)

^b Instituto de Investigaciones Bioquimicas Bahia Blanca   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA BUNGAROTOXIN; CARBACHOL; CHOLINERGIC RECEPTOR; ALANINE; MUSCARINIC M4 RECEPTOR; MUSCARINIC RECEPTOR;

AMINO ACID SUBSTITUTION; ARTICLE; CHANNEL GATING; HUMAN; HUMAN CELL; KIDNEY CELL; PRIORITY JOURNAL; RECEPTOR BINDING; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; ANIMAL; CELL CULTURE; CHEMISTRY; GENETICS; LIPID BILAYER; METABOLISM; MOLECULAR GENETICS; MOUSE; MUTATION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

ALANINE; AMINO ACID SEQUENCE; ANIMALS; CELLS, CULTURED; ION CHANNEL GATING; LIPID BILAYERS; MICE; MOLECULAR SEQUENCE DATA; MUTATION; RECEPTOR, MUSCARINIC M4; RECEPTORS, CHOLINERGIC; RECEPTORS, MUSCARINIC; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0031595748     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.54.1.146     Document Type: Article

References (33)

1. Baenziger JE and Méthot N (1995) Fourier transform infrared and hydrogen/deuterium exchange reveal an exchange-resistant core of α-helical peptide hydrogens in the nicotinic acetylcholine receptor. J Biol Chem 270:29129-29137.
2. Barrantes FJ (1993) Protein-lipid interactions, in New Comprehensive Biochemistry 26 (Watts A, ed) pp 231-257, Elsevier, Amsterdam.
3. Barrantes FJ (1997) Physical state of the nicotinic acetylcholine receptor membrane and modulation of the receptor channel by the lipid environment, in From Ion Channels to Cell-to-Cell Conversations (Latorre R and Saenz JC, eds) pp 199-216, Plenum Press, New York.
4. Blanton MP and Cohen JB (1992) Mapping the lipid-exposed regions in the Torpedo californica nicotinic acetylcholine receptor. Biochemistry 31:3738-3750.
5. Blanton MP and Cohen JB (1994) Identifying the lipid-protein interface of the Torpedo nicotinic acetylcholine receptor: secondary structure implications. Biochemistry 33:2859-2872.
6. Bouzat C and Barrantes FJ (1993) Acute exposure of nicotinic acetylcholine receptors to the synthetic glucocorticoid dexamethasone alters single-channel gating properties. Mol Neuropharm 3:109-116.
7. Bouzat C, Bren N, and Sine S (1994) Structural basis of the different gating kinetics of fetal and adult acetylcholine receptors. Neuron 13:1395-1402.
8. Bouzat C and Barrantes FJ (1996) Modulation of muscle nicotinic acetylcholine receptors by the glucocorticoid hydrocortisone: possible allosteric mechanism of channel blockade. J Biol Chem 271:25835-25841.
9. Bouzat C and Barrantes FJ (1997) Assigning functions to residues in the acetylcholine receptor channel region. Mol Membr Biol 14:167-177.
10. Cockroft VB, Osguthorpe DJ, Barnard EA, Friday AE, and Lunt GG (1992) Ligandgated ion channels: homology and diversity. Mol Neurobiol 4:129-169.
11. Fersht AR, Shi J-P, Knill-Jones J, Lowe DM, Wilkinson AJ, Blow DM, Brick P, Carter P, Waye MMY, and Winter G (1985) Hydrogen bonding and biological specificity analyzed by protein engineering. Nature (Lond) 314:235-238.
12. Filatov GN and White MM (1995) The role of conserved leucines in the in the M2 domain of the acetylcholine receptor in channel gating. Mol Pharmacol 48:379-384.
13. + channel pore through mutant cycles with a peptide inhibitor. Science (Washington DC) 268:307-310.
14. Horovitz A and Fersht AR (1990) Strategy for analysing the co-operativity of intramolecular interactions in peptides and proteins. J Mol Biol 214:613-617.
15. Hucho F, Tsetlin VI, and Machold J (1996) The emerging three-dimensional structure of a receptor. Eur J Biochem 239:539-557.
16. Jackson M (1993) Thermodynamics of Membrane Receptors and Channels. CRC Press, Boca Raton, FL.
17. Kyte J and Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105-132.
18. Lasalde JA, Tamamizu S, Butler DH, Vibat CRT, Hung B, and McNamee MG (1996) Tryptophan substitutions at the lipid-exposed transmembrane segment M4 of Torpedo californica acetylcholine receptor govern channel gating. Biochemistry 35:14139-14148.
19. Lee Y-H, Li L, Lasalde J, Rojas L, McNamee M, Ortiz-Miranda SI, and Pappone P (1994) Mutations in the M4 domain of Torpedo californica acetylcholine receptor dramatically alter ion channel function. Biophys J 66:646-653.
20. Li L, Schuchard M, Palma A, Pradier L, and McNamee MG (1990) Functional role of the Cys-451 thiol group in the M4 helix of the γ subunit of Torpedo californica acetylcholine receptor. Biochemistry 29:5428-5436.
21. Marsh D and Barrantes FJ (1978) Immobilized lipid in acetylcholine receptor-rich membranes from Torpedo marmorata. Proc Natl Acad Sci USA 75:4329-4333.
22. Orteils MO, Barrantes GE, Wood C, Lunt GG, and Barrantes FJ (1997) Molecular modelling of the nicotinic acetylcholine receptor transmembrane region in the open state. Prot Eng 10:511-517.
23. Ortells MO, Barrantes GE, and Barrantes FJ (1998) Molecular modelling of the nicotinic acetylcholine receptor, in The Nicotinic Acetylcholine receptor: Current Views and Future Trends (Barrantes FJ, ed.). pp 85-108, Neuroscience Intelligence Unit, Landes Publishing, Austin, TX.
24. Orteils MO and Lunt GG (1995) Evolutionary history of the ligand gated ion channel superfamily. Trends Neurosci 18:121-127.
25. Ortells MO and Lunt GG (1996) A β-sheet/α-helix model for the transmembrane region of the nicotinic acetylcholine receptor. Prot Eng 9:51-59.
26. Prince R and Sine SM (1998) The ligand binding domains of the nicotinic acetylcholine receptor, in The Nicotinic Acetylcholine receptor: Current Views and Future Trends (Barrantes FJ, ed.). pp 31-59, Neuroscience Intelligence Unit, Landes Publishing, Austin, TX.
27. Sigworth F and Sine SM (1987) Data transformations for improved display and fitting of single-channel dwell time histograms. Biophys J 52:1047-1052.
28. Sine SM (1993) Molecular dissection of subunit interfaces in the acetylcholine receptor: identification of residues that determine curare selectivity. Proc Nat Acad Sci USA 90:9436-9440.
29. Sine SM, Quiram P, Papanikolaou F, Kreienkamp HJ, and Taylor P (1994) Conserved tyrosines in the α subunit of the nicotinic acetylcholine receptor stabilize quaternary ammonium groups of agonist and curariform antagonists. J Biol Chem 269:8808-8816.
30. Sine SM and Taylor P (1979) Functional consequences of agonist-induced state transitions in the cholinergic receptor: studies in culture muscle cells. J Biol Chem 254:3315-3325.
31. Unwin N (1993) The nicotinic acetylcholine receptor at 9 Åresolution. J Membr Biol 229:1101-1124.
32. Unwin N (1995) Acetylcholine receptor channel imaged in the open state. Nature (Lond) 373:37-43.
33. Zhang Y, Chen J, and Auerbach A (1995) Activation of recombinant mouse acetylcholine receptors by acetylcholine, carbamylcholine and tetramethylammonium. J Physiol (Lond) 486.1:189-206.