Gentamicin uptake by LLCPK1 cells: Effect of intracellular and extracellular pH changes

Canadian Journal of Physiology and Pharmacology

Volumn 76, Issue 2, 1998, Pages 155-160

  Lui, Eric Chi Chung ^a   Bendayan, Reina ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Gentamicin; LLCPK1 cells; Nephrotoxicity; pH

Indexed keywords

CIMETIDINE; GENTAMICIN; QUINIDINE; QUININE; SODIUM AZIDE; VERAPAMIL; ANTIINFECTIVE AGENT; ENZYME INHIBITOR;

ACTIVE TRANSPORT; APICAL MEMBRANE; ARTICLE; CELL MEMBRANE; CELL PH; CONTROLLED STUDY; DRUG ACCUMULATION; DRUG TRANSPORT; DRUG UPTAKE; ENDOCYTOSIS; HUMAN; HUMAN CELL; IONIZATION; KIDNEY EPITHELIUM; KIDNEY PROXIMAL TUBULE; NEPHROTOXICITY; PRIORITY JOURNAL; PROTON TRANSPORT; TEMPERATURE DEPENDENCE; ANIMAL; CELL CULTURE; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; DRUG INTERACTION; METABOLISM; PH; SWINE; TEMPERATURE;

ANIMALS; ANTI-BACTERIAL AGENTS; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG INTERACTIONS; ENZYME INHIBITORS; GENTAMICINS; HYDROGEN-ION CONCENTRATION; KIDNEY TUBULES, PROXIMAL; SODIUM AZIDE; SWINE; TEMPERATURE;

EID: 0031595321     PISSN: 00084212     EISSN: None     Source Type: Journal    
DOI: 10.1139/cjpp-76-2-155     Document Type: Article

References (23)

1. 1 cells. J. Am. Soc. Nephrol. 5: 75-84.
2. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
3. Chiu, P.J.S., Miller, G.H., Long, J.F., and Waitz, J.A. 1979. Renal uptake and nephrotoxicity of gentamicin during urinary alkalinization in rats. Clin. Exp. Pharmacol. Physiol. 6: 317-326.
4. Holohan, P.D., and Ross, C.R. 1981. Mechanisms of organic cation transport in kidney plasma membrane vesicles. 2. ΔpH studies. J. Pharmacol. Exp. Ther. 216: 294-298.
5. 1). J. Pharmacol. Exp. Ther. 261(3): 1200-1205.
6. Hsu, C.H., Kurtz, T.W., Easterling, R.E., and Weller, J.M. 1974. Potentiation of gentamicin nephrotoxicity by metabolic acidosis. Proc. Soc. Exp. Biol. Med. 146: 894-897.
7. 1). Am. J. Physiol. 254: C251-C257.
8. 31P-NMR spectroscopy. Biochim. Biophys. Acta, 927: 203-212.
9. Josepovitz, C., Pastoriza-Munoz, E., Timmerman, D., Scott, M., Feldman, S., and Kaloyanides, G.J. 1982. Inhibition of gentamicin uptake in rat renal cortex in vivo by aminoglycosides and organic polycations. J. Pharmacol. Exp. Ther. 223: 314-321.
10. Lipsky, J.J., Cheng, L., Sacktor, B., and Lietman, P.S. 1980. Gentamicin uptake by renal tubule brush border membrane vesicles. J. Pharmacol. Exp. Ther. 215: 390-393.
11. Moore, R.D., Smith, C.R., Lipsky, J.J., Mellits, D.E., and Lietman, P.S. 1984. Risk factors for nephrotoxicity in patients treated with aminoglycosides. Ann. Intern. Med. 100: 352-357.
12. 1). J. Pharmacol. Exp. Ther. 238: 1071-1076.
13. Sastrasinh, M., Knauss, T.C., Weinberg, J.M., and Humes, H.D. 1982. Identification of the aminoglycoside binding site in rat renal brush border membranes. J. Pharmacol. Exp. Ther. 222(2): 350-358.
14. Schacht, J. 1979. Isolation of an aminoglycoside receptor from guinea pig inner ear tissues and kidney. Arch. Otorhinolaryngol. 224: 129-134.
15. 1) cells in culture. J. Pharmacol. Exp. Ther. 236: 254-262.
16. Smith, T.R., and Smith, C.R. 1982. Risk factors for aminoglycoside nephrotoxicity. In The aminoglycosides: microbiology, clinical use and toxicology. Edited by A. Welton. Marcel Dekker Inc., New York.
17. Sokol, P.P., Huiatt, K.R., Holohan, P.D., and Ross, C.R. 1989. Gentamicin and verapamil compete for a common transport mechanism in renal brush border membrane vesicles. J. Pharmacol. Exp. Ther. 251:927-942.
18. Takano, M., Inui, K.I., Okano, T., Saito, H., and Hori, R. 1984. Carrier mediated transport systems of tetraethylammonium in rat renal brush-border and basolateral membrane vesicles. Biochim. Biophys. Acta, 773: 113-124.
19. 1 kidney epithelial cell line. J. Pharmacol. Exp. Ther. 268(2): 669-674.
20. Walker, R.J., and Duggan, G.G. 1988. Drug nephrotoxicity. Annu. Rev. Pharmacol. Toxicol. 28: 331-345.
21. Williams, P.D. 1989. The application of renal cells in culture in studying drug-induced nephrotoxicity. In Vitro Cell Dev. Biol. 25(9): 800-805.
22. +-adenosine triphosphatase by gentamicin. J. Pharmacol. Exp. Ther. 231: 248-253.
23. Williams, P.D., Bennett, D.B., Gleason, C.R., and Hottendorf, G.H. 1987. Correlation between renal membrane binding and nephrotoxicities of aminoglycosides. Antimicrob. Agents Chemother. 31(4): 570-574.