Phospholipase A2 relieves phosphatidylcholine inhibition of micellar cholesterol absorption and transport by human intestinal cell line Caco-2

Journal of Lipid Research

Volumn 39, Issue 6, 1998, Pages 1197-1209

  Homan, Reynold ^a   Hamelehle, Katherine L ^a  

^a PFIZER INC   (United States)

Author keywords

Bile acid; Enterocyte; Fatty acid; FPL67047XX; Lysophosphatidylcholine; Micelle; Monoacylglycerol; Phospholipid; Retinol; Triacylglycerol

Indexed keywords

LYSOPHOSPHATIDYLCHOLINE; PHOSPHATIDYLCHOLINE; PHOSPHOLIPASE A2; 4 AMINOBUTYRIC ACID; ACYLGLYCEROL; BENZHYDRYL DERIVATIVE; CHOLESTEROL; DRUG DERIVATIVE; ENZYME INHIBITOR; FPL 67047XX; GLYCEROL OLEATE; OLEIC ACID; PHOSPHOLIPASE A; RETINOL; TAUROCHOLIC ACID;

ARTICLE; CHOLESTEROL ESTERIFICATION; CHOLESTEROL METABOLISM; CHOLESTEROL TRANSPORT; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTESTINE ABSORPTION; LIPID COMPOSITION; LIPID SOLUBILITY; LIPOLYSIS; MICELLE; PRIORITY JOURNAL; CELL STRAIN CACO 2; DRUG ANTAGONISM; DRUG EFFECT; INTESTINE MUCOSA; METABOLISM; PHYSIOLOGY;

BENZHYDRYL COMPOUNDS; CACO-2 CELLS; CHOLESTEROL; ENZYME INHIBITORS; GAMMA-AMINOBUTYRIC ACID; GLYCERIDES; HUMANS; INTESTINAL ABSORPTION; INTESTINAL MUCOSA; LYSOPHOSPHATIDYLCHOLINES; MICELLES; OLEIC ACID; PHOSPHATIDYLCHOLINES; PHOSPHOLIPASES A; TAUROCHOLIC ACID; VITAMIN A;

EID: 0031595208     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (46)

1. Chijiiwa, K. 1987. Distribution and partitioning of cholesterol and beta-sitosterol in micellar bile salt solutions. Am. J. Physiol. 253: G268-G273.
2. Grundy, S. M. 1983. Absorption and metabolism of dietary cholesterol. Annu. Rev. Nutr. 3: 71-96.
3. Thomson, A. B. R., and J. M. Dietschy. 1981. Intestinal lipid absorption: major extracellular and intracellular events. In Physiology of the Gastrointestinal Tract. L. R. Johnson, editor. Raven Press, New York. 1147-1220.
4. Thomson, A. B. R., M. Keelan, M. L. Garg, and M. T. Clandinin. 1989. Intestinal aspects of lipid absorption: in review. Can. J. Physiol. Pharmacol. 67: 179-191.
5. Carey, M. C., and D. M. Small. 1978. The physical chemistry of cholesterol solubility in bile. J. Clin. Invest. 61: 998-1026.
6. Rampone, A. J. 1973. The effect of lecithin on intestinal cholesterol uptake by rat intestine in vitro. J. Physiol. 229: 505-514.
7. Rodgers, J. B., and P. J. O'Connor. 1975. The effects of phosphatidylcholine on fatty acid and cholesterol absorption from mixed micellar solutions. Biochim. Biophys. Acta. 409: 192-200.
8. Rampone, A. J., and L. R. Long. 1977. The effect of phosphatidylcholine and lysophosphatidylcholine on the absorption and mucosal metabolism of oleic acid and cholesterol in vitro. Biochim. Biophys. Acta. 486: 500-510.
9. Thomson, A. B. R., and L. Cleland. 1981. Intestinal cholesterol uptake from phospholipid vesicles and from simple and mixed micelles. Lipids. 16: 881-887.
10. Rampone, A. J., and C. M. Machida. 1981. Mode of action of lecithin in suppressing cholesterol absorption. J. Lipid Res. 22: 744-752.
11. Reynier, M. O., H. Lafont, C. Crotte, P. Sauve, and A. Gerolami. 1985. Intestinal cholesterol uptake: comparison between mixed micelles containing lecithin or lysolecithin. Lipids. 20: 145-150.
12. Hollander, D., and D. Morgan. 1980. Effect of plant sterols, fatty acids and lecithin on cholesterol absorption in vivo in the rat. Lipids. 15: 395-400.
13. Proulx, P., H. Aubry, I. Brglez, and D. G. Williamson. 1984. The effect of phosphoglycerides on the incorporation of cholesterol into isolated brush-border membranes from rabbit small intestine. Biochim. Biophys. Acta. 775: 341-346.
14. Kinugasa, K., Y. Yamane, K. Adachi, K. Kataoka, M. Kato, T. Sasaki, H. Yorizumi, A. Adachi, Y. Horii, O. Morinaga, Y. Inada, K. Kashima, and T. Takino. 1988. Effect of phosphatidylcholine on uptake of lipids into isolated rat enterocytes from mixed micelles. J. Kyoto Pref. Univ. Med. 97: 651-659.
15. Arnesjö, B., Å. Nilsson, J. Barrowman, and B. Borgström. 1969. Intestinal digestion and absorption of cholesterol and lecithin in the human. Scand. J. Gastroenterol. 4: 653-665.
16. Clark, S. B. 1978. Chylomicron composition during duodenal triglyceride and lecithin infusion. Am. J. Physiol. 235: E183-E190.
17. Rodgers, J. B., J. D. Fondacaro, and J. Kot. 1977. The effect of synthetic diether phospholipid on lipid absorption in the rat. J. Lab. Clin. Med. 89: 147-152.
18. Greten, H., H. Raetzer, A. Stiehl, and G. Schettler. 1980. The effect of polyunsaturated phosphatidylcholine on plasma lipids and fecal sterol excretion. Atherosclerosis. 36: 81-88.
19. Kesäniemi, Y. A., and S. M. Grundy. 1986. Effects of dietary polyenylphosphatidylcholine on metabolism of cholesterol and triglycerides in hypertriglyceridemic patients. Am. J. Clin. Nutr. 43: 98-107.
20. Beil, F. U., and S. M. Grundy. 1980. Studies on plasma lipoproteins during absorption of exogenous lecithin in man. J. Lipid Res. 21: 525-536.
21. Levy, E., M. Mehran, and E. Seidman. 1995. Caco-2 cells as a model for intestinal lipoprotein synthesis and secretion. FASEB J. 9: 626-635.
22. Field, F. J., and S. N. Mathur. 1995. Intestinal lipoprotein synthesis and secretion. Prog. Lipid Res. 34: 185-198.
23. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
24. Slayback, J. R. B., L. W. Y. Cheung, and R. P. Beyer. 1977. Quantitative extraction of microgram amounts of lipid from cultured human cells. Anal. Biochem. 83: 372-384.
25. Christie, W. W. 1985. Rapid separation and quantification of lipid classes by high performance liquid chromatography and mass (light-scattering) detection. J. Lipid Res. 26: 507-512.
26. Kessler, M. J. 1989. Liquid Scintillation Analysis. Packard Instrument Company, Meriden, CT.
27. Homan, R., and M. K. Anderson. 1998. Rapid separation and quantitation of combined neutral and polar lipid classes by high-performance liquid chromatography and evaporative light-scattering mass detection. J. Chromatogr. In press.
28. Roda, A., C. Cerrè, P. Simoni, C. Polimeni, C. Vaccari, and A. Pistillo. 1992. Determination of free and amidated bile acids by high-performance liquid chromatography with evaporative light-scattering mass detection. J. Lipid Res. 33: 1393-1402.
29. Staggers, J. E., O. Hernell, R. J. Stafford, and M. C. Carey. 1990. Physical-chemical behavior of dietary and biliary lipids during intestinal digestion and absorption. 1. Phase behavior and aggregation states of model lipid systems patterned after aqueous duodenal contents of healthy adult human beings. Biochemistry. 29: 2028-2040.
30. Cohen, D. E., and M. C. Carey. 1990. Rapid (1 hour) high performance gel filtration chromatography resolves coexisting sample micelles, mixed micelles, and vesicles in bile. J. Lipid Res. 31: 2103-2112.
31. Blomhoff, R., M. H. Green, T. Berg, and K. R. Norum. 1990. Transport and storage of vitamin A. Science. 250: 399-404.
32. Hernell, O., J. E. Staggers, and M. C. Carey. 1990. Physical-chemi-cal behavior of dietary and biliary lipids during intestinal digestion and absorption. 2. Phase analysis and aggregation states of luminal lipids during duodenal fat digestion in healthy adult human beings. Biochemistry. 29: 2041-2056.
33. 2. J. Med. Chem. 37: 557-559.
34. Krause, B. R., M. Anderson, C. L. Bisgaier, T. Bocan, R. Bousley, P. DeHart, A. Essenburg, K. Hamelehle, R. Homan, K. Kieft, W. McNally, R. Stanfield, and R. S. Newton. 1993. In vivo evidence that the lipid-regulating activity of the ACAT inhibitor CI-976 in rats is due to inhibition of both intestinal and liver ACAT. J. Lipid Res. 34: 279-294.
35. Mathur, S. N., E. Born, S. Murthy, and F. J. Field. 1996. Phosphatidylcholine increases the secretion of triacylglycerol-rich lipoproteins by Caco-2 cells. Biochem. J. 314: 569-575.
36. Field, F. J., E. Born, H. Chen, S. Murthy, and S. N. Mathur. 1994. Regulation of apolipoprotein B secretion by biliary lipids in Caco-2 cells. J. Lipid Res. 35: 749-762.
37. Field, F. J., E. Born, H. Chen, S. Murthy, and S. N. Mathur. 1994. Lysophosphatidylcholine increases the secretion of cholesteryl ester-poor triacylglycerol-rich lipoproteins by CaCo-2 cells. Biochem. J. 304: 35-42.
38. Phillips, M. C., W. J. Johnson, and G. H. Rothblat. 1987. Mechanisms and consequences of cellular cholesterol exchange and transfer. Biochim. Biophys. Acta. 906: 223-276.
39. Bittman, R. 1993. A review of the kinetics of cholesterol movement between donor and acceptor bilayer membranes. In Cholesterol in Membrane Models. L. Finegold, editor. CRC Press, Boca Raton. 46-61.
40. Pownall, H. J., D. L. Hickson, and L. C. Smith. 1983. Transport of biological lipophiles: effect of lipophile structure. J. Am. Chem. Soc. 105: 2440-2445.
41. Lütjohann, D., I. Björkhem, U. F. Beil, and K. von Bergmann. 1995. Sterol absorption and sterol balance in phytosterolemia evaluated by deuterium-labeled sterols: effect of sitostanol treatment. J. Lipid Res. 36: 1763-1773.
42. Kan, C-C., and R. Bittman. 1991. Spontaneous rates of sitosterol and cholesterol exchange between phospholipid vesicles and between lysophospholipid dispersions: evidence that desorption rate is impeded by the 24α-ethyl group of sitosterol. J. Am. Chem. Soc. 113: 6650-6656.
43. Homan, R., and B. R. Krause. 1997. Established and emerging strategies for inhibition of cholesterol absorption. Curr. Pharm. Design. 3: 29-44.
44. Northfield, T. C., and A. F. Hofmann. 1975. Biliary lipid output during three meals and an overnight fast. Gut. 16: 1-17.
45. 2-facilitated cholesterol uptake into intestinal Caco-2 cells. J. Biol. Chem. 272: 13380-13389.
46. O'Connor, P. J., T. A. Loiudice, W. Bochenek, and J. B. Rodgers. 1978. Effect of diester and diether phosphatidylcholine on intestinal absorption of neutral and acidic sterols. Digest. Dis. 23: 316-320.