Compartmentation of [14C]glutamate and [14C]glutamine oxidative metabolism in the rat hippocampus as determined by microdialysis

Journal of Neurochemistry

Volumn 71, Issue 3, 1998, Pages 1315-1320

  Zielke, H Ronald ^a,b   Collins Jr , Roger M ^a   Baab, Peter J ^a   Huang, Yinyin ^a   Zielke, Carol L ^a   Tyson Tildon, J ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

Author keywords

Brain energy metabolism; Glutamate; Glutamine; Microdialysis; Oxidation

Indexed keywords

CARBON 14; CARBON DIOXIDE; GLUCOSE; GLUTAMIC ACID; GLUTAMINE; CARBON;

AEROBIC METABOLISM; AMINO ACID METABOLISM; ANIMAL EXPERIMENT; ARTICLE; CEREBROSPINAL FLUID; CONTROLLED STUDY; ENERGY METABOLISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIPPOCAMPUS; MALE; MICRODIALYSIS; NONHUMAN; PRIORITY JOURNAL; RAT; ANIMAL; EXTRACELLULAR SPACE; METABOLISM; OXIDATION REDUCTION REACTION;

ANIMALIA; HIPPOCAMPUS HIPPOCAMPUS;

ANIMALS; CARBON DIOXIDE; CARBON RADIOISOTOPES; EXTRACELLULAR SPACE; GLUTAMIC ACID; GLUTAMINE; HIPPOCAMPUS; MALE; MICRODIALYSIS; OXIDATION-REDUCTION; RATS;

EID: 0031595166     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1998.71031315.x     Document Type: Article

References (28)

1. Balázs R., Patel J. J., and Richter D. (1973 ) Metabolic compartments in the brain: their properties and relations to morphological structures, in Metabolic Compartmentation in the Brain ( Balázs R. and Cremer J. E., eds). pp. 167-184. Macmillan, London.
2. Berl S. and Clarke D. D. (1969) Metabolic compartmentation of glutamate in the CNS, in Handbook of Neurochemistry: Chemical and Cellular Architecture, Vol. 1 (Lajtha A., ed), pp. 447-472. Plenum Press, New York.
3. Bittar P. G., Charnay Y., Pellerin L., Bouras C., and Magistretti P. J. (1996) Selective distribution of lactate dehydrogenase isoenzymes in neurons and astrocytes of human brain. J. Cereb. Blood Flow Metab. 16, 1079-1089.
4. Butterworth R. F. (1983) Metabolism of glutamate and related amino acids in insulin hypoglycemia, in Glutamine, Glutamate and GABA in the Central Nervous System (Hertz L., Kvamme E., McGeer E. G., and Schousboe A., eds), pp. 595-608. Alan R. Liss, Inc., New York.
5. Cremer J. E. (1981) Nutrients for the brain: problems in supply. Early Hum. Dev. 5, 117-132.
6. 13C] glucose. J. Neurochem. 63, 1377-1385.
7. Hawkins R. A., Williamson D. H., and Krebs H. A. (1971) Ketone body utilization by adult and suckling rat brain in vivo. Biochem. J. 122, 13-18.
8. Hertz L. and Schousboe A. (1988) Metabolism of glutamate and glutamine in neurons and astrocytes in primary cultures, in Glutamine and Glutamate in Mammals, Vol. II (Kvamme K., ed), pp. 39-55. CRC Press, Boca Raton, Florida.
9. 2 by brain microdialysis. Dev. Neurosci. 15, 233-239.
10. Itoh T. and Quastel J. H. (1970) Acetoacetate metabolism in infant and adult rat brain in vitro. Biochem. J. 116, 641-655.
11. McKenna M. C., Sonnenwald U., Huang X., Stevenson J. H., and Zielke H. R. (1996a) Exogenous glutamate concentration regulates the metabolic fate of glutamate in astrocytes. J. Neurochem. 66, 386-393.
12. McKenna M. C., Tildon J. T., Stevenson J. H., and Huang X. (1996b) New insights into the compartmentation of glutamate and glutamine in cultured rat brain astrocytes. Dev. Neurosci. 18, 380-390.
13. Nicklas W. J. (1986) Glial-neuronal interrelationships in the metabolism of excitatory amino acids, in Excitatory Amino Acids (Roberts P. J., Storm-Mathisen J., and Bradford H., eds), pp. 595-608. Macmillan Press, London.
14. Norenberg M. D. and Martinez-Hernandez A. (1979) Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res. 161, 303-310.
15. Pellerin L. and Magistretti P. J. (1994) Glutamate uptake into astrocytes stimulates aerobic glycolysis: a mechanism coupling neuronal activity to glucose utilization. Proc. Natl Acad. Sci. USA 91, 10625-10629.
16. Roeder L. M., Tildon J. T., and Holman D. C. (1984) Competition among oxidizable substrates in brains of young and adult rats. Dissociated cells. Biochem. J. 219, 131-135.
17. Schousboe A., Drejer J., and Hertz L. (1988) Uptake and release of glutamine in neurons and astrocytes in primary cultures, in Glutamine and Glutamate in Mammals, Vol. II (Kvamme K., ed), pp. 21-38. CRC Press, Boca Raton, Florida.
18. Sendelbeck L. (1987) Recipe for preparation of artificial CSF, in Special Delivery, Vol. 8(3) (Ray N., ed), p. 4. ALZA Corp., Palo Alto, California.
19. Shank R. P. and Campbell G. L. (1983) Glutamate, in Handbook of Neurochemistry: Metabolism in the Nervous System, Vol. 3 (Lajtha A., ed), pp. 381-404. Plenum Press, New York.
20. 13C] glucose metabolism. J. Neurochem. 61, 315-323.
21. Sokoloff L. (1960) The metabolism of the central nervous system in vivo, in Handbook of Physiology-Neurophysiology, Vol. 3 (Field J., Magoun H. W., and Hall V. E., eds), pp. 1843-1864. American Physiological Society, Washington, D.C.
22. Tildon J. T. (1983) Glutamine: a possible energy source for the brain, in Glutamine, Glutamate and GABA in the Central Nervous System (Hertz L., Kvamme E., McGeer E. G., and Schousboe A., eds). pp. 415-430. Alan R. Liss, Inc., New York.
23. Waniewski R. A. and Martin D. L. (1986) Exogenous glutamate is metabolized to glutamine and exported by rat primary astrocyte cultures. J. Neurochem. 47, 304-313.
24. 14C-labeled glutamate in astrocytes in primary cultures. J. Neurochem. 39, 954-960.
25. 14C] glutamine in mouse cerebral neurons in primary cultures. J. Neurosci. Res. 11, 351-357.
26. Zielke H. R., Tildon J. T., Landry M. E., and Max S. R. (1990) Effect of 8-bromo-cAMP and dexamethasone on glutamate metabolism in rat astrocytes. Neurochem. Res. 15, 1115-1122.
27. Zielke H. R., Tildon J. T., and Zielke C. L. (1996) Use of intracellular versus extracellular specific activities in calculation of glutamine metabolism in astrocytes: effect of dibutyryl cyclic AMP. Dev. Neurosci. 18, 224-230.
28. Zielke H. R., Huang Y., Baab P. J., Collins R. M. Jr., Zielke C. L., and Tildon J. T. (1997) Effect of α-ketoisocaproate and leucine on the in vivo oxidation of glutamate and glutamine in the rat brain. Neurochem. Res. 22, 1159-1164.