Giardia infestinalis: Characterization of a NADP-dependent glutamate dehydrogenase

Experimental Parasitology

Volumn 88, Issue 2, 1998, Pages 131-138

  Park, Jeong Hyun ^a,b,c   Schofield, Philip J ^a   Edwards, Michael R ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

^c UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 OXOGLUTARIC ACID; GLUTAMATE DEHYDROGENASE (NADP); REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANIMAL EXPERIMENT; ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; GIARDIA LAMBLIA; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; ANIMAL; CHEMISTRY; ENZYMOLOGY; GEL CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANIMALIA; GIARDIA; GIARDIA INTESTINALIS; PROTOZOA;

ANIMALS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GIARDIA LAMBLIA; GLUTAMATE DEHYDROGENASE (NADP+); HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0031594465     PISSN: 00144894     EISSN: None     Source Type: Journal    
DOI: 10.1006/expr.1998.4199     Document Type: Article

References (36)

1. Allmaier, G., Schäffer, C., Messner, P., Rapp, U., and Mayer-Posner, F. J. 1995. Accurate determination of the molecular weight of the major surface layer protein isolated from Clostridium thermosaccharolyticum by time-of-flight mass spectrometry. Journal of Bacteriology 177, 1402-1404.
2. Beavis, R. C., and Chait, B. T. 1989. Cinnamic acid derivatives as matrices for ultraviolet laser desorption mass spectrometry of proteins. Rapid Communications in Mass Spectrometry 3, 432-435.
3. Blum, J. J. 1994. Energy metabolism in Leishmania. Journal of Bioenergetics and Biomembranes 26, 147-155.
4. Brun, A., Chalot, M., Botton, B., and Martin, F. 1992. Purification and characterization of glutamine synthetase and NADP-glutamate dehydrogenase from the ectomycorrhizal fungus Laccaria laccata. Plant Physiology 99, 938-944.
5. Bursell, J. D. H., Kirk, J., Hall, S. T., Gero, A. M., and Kirk, K. 1996. Volume-regulatory amino acid release form the protozoan parasite Crithidia luciliae. Journal of Membrane Biology 154, 131-141.
6. Carneiro, V. T. C., and Caldas, R. A. 1983. Regulatory studies of L-glutamate dehydrogenase from Trypanosoma cruzi epimastigotes. Comparative Biochemistry and Physiology 75B, 61-64.
7. Cazzulo, J. J., Nowicki, C., Santome, J. A., Wernstedt, C., and Hellman, U. 1988. Amino acid composition and N-terminal sequence of the NADP-linked glutamate dehydrogenase from Trypanosoma cruzi. FEMS Microbiology Letter 56, 215-220.
8. Cleland, W. W. 1979. Statistical analysis of enzyme kinetic data. Methods in Enzymology 63, 103-138.
9. Consalvi, V., Chiaraluce, R., Politi, L., Gambacorta, A., De Rosa, M., and Scandurra, R. 1991a. Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus. European Journal of Biochemistry 196, 459-467.
10. Consalvi, V., Chiaraluce, R., Politi, L., Vaccaro, R., De Rosa, M., and Scandurra, R., 1991b. Extremely thermostable glutamate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus furiosus. European Journal of Biochemistry 202, 1189-1196.
11. Cronshaw, A. D., Fothergill-Gilmore, L. A., and Hulmes, D. J. S. 1995. The proteolytic processing site of the precursor of lysyl oxidase. Biochemical Journal 206, 279-284.
12. Darling, T. N., Burrows, C. M., and Blum, J. J. 1990. Rapid shape change and release of ninhydrin-positive substances by Leishmania major promastigotes in response to hypo-osmotic stress. The Journal of Protozoology 37, 493-499.
13. +-dependent glutamate dehydrogenase from the facultative methylotroph Hyphomicrobium X. FEMS Microbiology Letter 48, 133-137.
14. Duschak, V. G., and Cazzulo, J. J. 1991. Subcellular localization of glutamate dehydrogenase and alanine aminotransferase in epimastigotes of Trypanosoma cruzi. FEMS Microbiology Letter 83, 131-136.
15. Edwards, M. R., Gilroy, F. V., Jimenez, B. M., and O'Sullivan, W. J. 1989. Alanine is a major end product of metabolism by Giardia lamblia: A proton nuclear magnetic resonance study. Molecular and Biochemical Parasitology 37, 19-26.
16. Gore, M. G. 1981. L-Glutamate dehydrogenase. International Journal of Biochemistry 13, 879-886.
17. Hudson, R. C., and Daniel, R. M. 1993. L-Glutamate dehydrogenases: Distribution, properties and mechanism. Comparative Biochemistry and Physiology 106B, 767-792.
18. Karas, M., and Bahr, U. 1991. Matrix-assisted laser desorption ionization mass spectrometry. Mass Spectrometry Reviews 10, 335-357.
19. Knodler, L. A., Edwards, M. R., and Schofield, P. J. 1994. The intracellular amino acid pools of Giardia intestinalis, Trichomonas vaginalis and Crithidia luciliae. Experimental Parasitology 79, 117-125.
20. Knodler, L. A., Edwards, M. R., and Schofield, P. J. 1995. Arginine transport and metabolism in Giardia intestinalis support its position as a transition between the prokaryotic and eukaryotic kingdoms. Microbiology 141, 2063-2070.
21. +-dependent glutamate dehydrogenases in Nitrobacter agilis. Journal of General Microbiology 130, 967-973.
22. Lowry, O. H., Rosebrough, N. H., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Polin phenol reagent. Journal of Biological Chemistry 193, 265-275.
23. O'Daly, J. A., Serrano, L. E., and Rodrigues, M. B. 1983. Free amino acid pool and proteolytic enzymes in Trypanosoma cruzi cultured in vitro. International Journal for Parasitology 13, 433-440.
24. Op Den Camp, H. J. M., Liem, K. D., Meesters, P., Hermans, J. M. H., and Van Der Drift, C. 1989. Purification and characterization of the NADP-dependent glutamate dehydrogenase from Bacillus fastidiosus. Antonie van Leeuwenhoek 55, 303-311.
25. Paget, T. A., Kelly, M. L., Jarroll, E. L., Lindmark, D. G., and Lloyd, D. 1993. The effects of oxygen on fermentation in Giardia lamblia. Molecular and Biochemical Parasitology 57, 65-72.
26. Park, J. H., Schofield, P J., and Edwards, M. R. 1995. The role of alanine in the acute response of Giardia intestinalis to hypo-osmotic shock. Microbiology 141, 2455-2462.
27. Park, J. H., Schofield, P. J., and Edwards, M. R. 1997. Giardia intestinalis: Volume recovery in response to cell swelling. Experimental Parasitology 86, 19-28.
28. Schofield, P. J., Edwards, M. R., Matthews, J., and Wilson, J. R. 1992. The pathway of arginine catabolism in Giardia intestinalis. Molecular and Biochemical Parasitology 51, 29-36.
29. Simon, M. W., Jayasimhulu, K, and Mukkada, A. J. 1983. The free amino acid pool in Leishmania tropica promastigotes. Molecular and Biochemical Parasitology 9, 47-59.
30. Sogin, M. L., Gunderson, J. H., Elwood, H. J., Alonso, R. A., and Peattie, D. A. 1989. Phylogenetic meaning of the kingdom concept: An unusual ribosomal RNA from Giardia lamblia. Science 243, 75-77.
31. Turner, A. C., and Lushbaugh, W. B. 1988. Trichomonas vaginalis: Characterisation of its glutamate dehydrogenase. Experimental Parasitology 67, 47-53.
32. Walter, R. D., and Ebert, F. 1979. Evidence for NADH- and NADPH-linked glutamate dehydrogenase in Trypanosoma cruzi epimastigotes. The Journal of Protozoology 26, 653-656.
33. Whitaker, J. R., and Granum, P. E. 1980. An absolute method for protein determination based on difference in absorbance at 235 and 280 nm. Analytical Biochemistry 109, 156-159.
34. Yamamoto, I., Abe, A., Saito, H., and Ishimoto, M. 1984. The pathway of ammonia assimilation in Bacteroides fragilis. Journal of General and Applied Microbiology 30, 499-508.
35. Yamamoto, I., Abe, A., and Ishimoto, M. 1987. Properties of glutamate dehydrogenase purified from Bacteroides fragilis. Journal of Biochemistry 101, 1391-1397.
36. Yee, J., and Dennis, P. P. 1992. Isolation and characterization of a NADP-dependent glutamate dehydrogenase gene from the primitive eucaryote Giardia lamblia. Journal of Biological Chemistry 267, 7539-7544.