Immunolocalization of myosin Iβ in the hair cell's hair bundle

Cell Motility and the Cytoskeleton

Volumn 39, Issue 2, 1998, Pages 159-165

  Metcalf, Anne B ^a,b  

^a ROCKEFELLER UNIVERSITY   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Adaptation; Auditory system; Molecular motor; Vestibular system

Indexed keywords

MYOSIN; POLYCLONAL ANTIBODY;

ACOUSTICS; ADAPTATION; ARTICLE; AUDITORY SYSTEM; HAIR CELL; HUMAN; HUMAN CELL; IMMUNOELECTRON MICROSCOPY; IMMUNOREACTIVITY; PRIORITY JOURNAL; PROTEIN LOCALIZATION; VESTIBULAR SYSTEM; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; MOLECULAR GENETICS; RABBIT; ULTRASTRUCTURE;

AMINO ACID SEQUENCE; ANIMALS; HAIR CELLS; MOLECULAR SEQUENCE DATA; MYOSINS; RABBITS;

EID: 0031594148     PISSN: 08861544     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0169(1998)39:2<159::AID-CM6>3.0.CO;2-1     Document Type: Article

References (20)

1. Assad, J.A., and Corey D.P. (1992): An active motor model for adaptation by vertebrate hair cells. J. Neurosci. 12:3291-3309.
2. Fisher, A.J., Smith, C.A., Thoden, J., Smith, R., Sutoh, K., Holden, H.M., and Rayment, I. (1995): Structural studies of myosin: Nucleotide complexes: A revised model for the molecular basis of muscle contraction. Biophys. J. 68, 19s-28s.
3. Gillespie, P.G., and Hudspeth, A.J. (1993): Adenine nucleotide diphosphates block adaptation of mechanoelectrical transduction in hair cells. Proc. Natl. Acad, Sci. U.S.A. 90:2710-2714.
4. Gillespie, P.C., Wagner, M.C., and Hudspeth, A.J. (1993): Identification of a 120 kD hair-bundle myosin located near stereociliary tips. Neuron 11:581-594.
5. Hasson, T., Gillespie, P.G., Garcia, J.A., MacDonald, R.B., Zhao, Y.-D., Yea, A.C., Mooseker, M.S., and Corey, D.P. (1997): J. Cell Biol. 137:1287-1307.
6. Hirokawa, N., and Tilney, L.G. (1982): Interactions between actin filaments and between actin filaments and membranes in quick-frozen and deeply etched hair cells of the chick ear. J. Cell Biol. 95:249-261.
7. Howard, J., and Hudspeth A.J. (1987); Mechanical relaxation of the hair bundle mediates adaptation in mechanoelectrical transduction by the bullfrog's saccular hair cell. Proc. Natl. Acad. Sci. U.S.A. 84:3064-3068.
8. Howard, J., and Hudspeth A.J. (1988): Compliance of the hair bundle associated with the gating of mechanoelectrical transduction channels in the bullfrog's saccular hair cell. Neuron 1:189-199.
9. Hudspeth, A.J., and Gillespie, P.G. (1994): Pulling springs to tune transduction: adaptation by hair cells. Neuron 12:1-9.
10. Jacobs, R.A., and Hudspeth, A.J. (1990): Ultrastructural correlates of mechanoelectrical transduction in hair cells of the bullfrog's internal ear. Cold Spring Harb. Symp. Quant. Biol. 55:547-561.
11. 2+ entry through mechanosensitive channels localizes the site of mechanoelectrical transduction in hair cells. Proc. Natl. Acad. Sci. U.S.A. 92:1506-1509.
12. Metcalf, A.B. (1995): Molecular characterization of amphibian myosin Iβ, a candidate for the hair bundle's adaptation motor. Thesis, University of Texas Southwestern Medical Center. Dallas. TX.
13. Metcalf, A.B., Chelliah, Y., and Hudspeth, A.J. (1994): Molecular cloning of a myosin Iβ isozyme that may mediate adaptation by hair cells of the bullfrog's internal ear. Proc. Natl. Acad. Sci. U.S.A. 91:11821-11825.
14. Rayment, I., Holden, H.M., Whittaker, M., Yohn, C.B., Lorenz, M., Holmes, K.C., and Milligan, R.A. (1993a): Structure of the actin-myosin complex and its implications for muscle contraction. Science 261:58-65.
15. Rayment, I., Rypniewski, W.R., Schmidt-Base, K., Smith, R., Tomchick, P.R., Benning, M.M., Winkelmann, D.A., Wesenberg, G., and Holden, H.M. (1993b): Three dimensional structure of myosin subfragment-1: A molecular motor. Science 261:50-58.
16. Reizes, O., Barylko, B., Li, C., Südhof, T.C., and Albanesi, J.P. (1994): Domain structure of a mammalian myosin Iβ. Proc. Natl. Acad. Sci. U.S.A. 91:6349-6353.
17. Schroder, R.R., Manstein, D.J., Jahn, W., Holden, H., Rayment, I., Holmes, K.C., and Spudich, J.A. (1993): Three-dimensional atomic model of F-actin decorated with Dictyostelium myosin S1. Nature 364:171-174.
18. Sole, C.K., Derfler, B.H., Duyk, G.M., and Corey D.P. (1994): Molecular cloning of myosins from the bullfrog saccular macula: A candidate for the hair cell adaptation motor. Aud. Neurosci. 1:63-75.
19. Wagner, M.C., Barylko, B., and Albanesi, J.P (1992): Tissue distribution and subcellular localization of mammalian myosin I. J. Cell Biol. 119:163-170.
20. Yamoah, E.N., and Gillespie, P.G. (1996): Phosphate analogs block adaptation in hair cells by inhibiting adaptation-motor force production. Neuron 17:523-533.