Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

Marine Ecology Progress Series

Volumn 161, Issue , 1997, Pages 303-307

  Barlow, R G ^a,b   Cummings, D G ^a   Gibb, S W ^a  

^a PLYMOUTH MARINE LABORATORY   (United Kingdom)

^b MARINE AND COASTAL MANAGEMENT   (South Africa)

Author keywords

C 8 column; Carotenoids; Chlorophylls; HPLC; Improved resolution

Indexed keywords

CHLOROPHYLLS; CHROMATOGRAPHIC TECHNIQUES; LUTEIN; PHYTOPLANKTON; SAMPLING; ZEAXANTHIN;

EID: 0031593009     PISSN: 01718630     EISSN: None     Source Type: Journal    
DOI: 10.3354/meps161303     Document Type: Article

References (19)

1. Barlow RG, Mantoura RFC, Cummings DG, Fileman TW (1997a) Pigment chemotaxonomic distributions of phytoplankton during summer in the western Mediterranean. Deep Sea Res II 44:833-850
2. Barlow RG, Mantoura RFC, Cummings DG (1997b) Monsoonal influence on the distribution of phytoplankton pigments in the Arabian Sea. Deep Sea Res II (in press)
3. Chisholm SW, Olson RJ, Zettler ER, Goericke R, Waterbury JB, Welschmeyer NA (1988) A novel free-living prochlorophyte abundant in the oceanic euphotic zone. Nature 334: 340-343
4. Everitt DA, Wright SW, Volkman JK, Thomas DP, Lindstrom EJ (1990) Phytoplankton community compositions in the western equatorial Pacific determined from chlorophyll a and carotenoid pigment distributions. Deep Sea Res 37: 975-997
5. Garrido JL, Zapata M (1993a) High performance liquid chromatographic separation of polar and non-polar chlorophyll pigments from algae using a wide pore polymeric octadecyl silica column. J High Res Chrom 16:229-233
6. 2 and a and of carotenoids of chromophyte algae on a polymeric octadecyl silica column. Chromatographia 35:543-547
7. Gieskes WW, Kraay GW (1983) Unknown chlorophyll a derivatives in the North Sea and the tropical Atlantic Ocean revealed by HPLC analysis. Limnol Oceanogr 28:757-766
8. Goericke R, Repeta DJ (1992) The pigments of Prochlorococcus marinus: the presence of divinyl chlorophyll a and b in a marine procaryote. Limnol Oceanogr 37:425-433
9. Goericke R, Repeta DJ (1993) Chlorophylls a and b and divinyl chlorophylls a and b in the open subtropical North Atlantic Ocean. Mar Ecol Prog Ser 101:307-313
10. Jeffrey SW, Mantoura RFC, Wright SW (eds) (1997) Phytoplankton pigments in oceanography: guidelines to modern methods. UNESCO, Paris
11. 3 of marine phytoplankton by reversed-phase C18 high performance liquid chromatography. J Phycol 28:708-712
12. Letelier RM, Bidigare RR, Hebel DV, Ondrusek M, Winn CD, Karl DM (1993) Temporal variability of phytoplankton community structure based on pigment analysis. Limnol Oceanogr 38:1420-1437
13. Mantoura RFC, Llewellyn CA (1983) The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high performance liquid chromatography. Analyt China Acta 151:297-314
14. Van Heukelem L, Lewitus AJ, Kana TM, Craft NE (1992) High performance liquid chromatography of phytoplankton pigments using a polymeric reversed phase C18 column. J Phycol 28:867-872
15. Van Heukelem L, Lewitus AJ, Kana TM, Craft NE (1994) Improved separations of phytoplankton pigments using temperature-controlled high performance liquid chromatography. Mar Ecol Prog Ser 114:303-313
16. Van Lenning K, Garrido JL, Aristegui J, Zapata M (1995) Temperature programmed high performance liquid chromatography separation of mono-and divinyl chlorophyll forms from marine phytoplankton. Chromatographia 41: 539-543
17. Vidussi F, Claustre H, Bustillos-Guzmàn J, Cailliau C, Marty JC (1996) Determination of chlorophylls and carotenoids of marine phytoplankton: separation of chlorophyll a from divinyl chlorophyll a and zeaxanthin from lutein. J Plankton Res 18:2377-2382
18. Wright SW, Jeffrey SW, Mantoura RFC, Llewellyn CA, Bjornland T, Repeta D, Welschmeyer N (1991) Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Mar Ecol Prog Ser 77: 183-196
19. Zapata M, Garrido JL (1997) Occurrence of phytylated chlorophyll c in Isochrysis galbana and Isochrysis sp. (clone t-iso) (Prymnesiophyceae). J Phycol 33:209-214