Effects of mutations in Pr160(gag-pol) upon tRNA(Lys3) and Pr160(gag-pol) incorporation into HIV-1

Journal of Molecular Biology

Volumn 265, Issue 4, 1997, Pages 419-431

  Mak, Johnson ^a,b   Khorchid, Ahmad ^a,b   Cao, Qun ^a   Huang, Yue ^a,b   Lowy, Israel ^c   Parniak, M A ^a,b   Prasad, Vinayaka R ^d   Wainberg, Mark A ^a,b   Kleiman, Lawrence ^a,b  

^a MCGILL UNIVERSITY   (Canada)

^b MCGILL UNIVERSITY   (Canada)

^c Och Spine at New York Presbyterian Hospitals   (United States)

^d ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

HIV 1; Pr160(gag pol); Protein processing; tRNA(Lys3)

Indexed keywords

AMINO ACID; GAG PROTEIN; INTEGRASE; LYSINE TRANSFER RNA; MUTANT PROTEIN; POL PROTEIN; PROTEIN PRECURSOR; RIBONUCLEASE H; VIRUS RNA; AMINOACYL TRANSFER RNA; PR160 GAG POL PRECURSOR PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; PR160 GAG-POL PRECURSOR PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; RNA DIRECTED DNA POLYMERASE; TRNA, LYSINE; TRNA, LYSINE-;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; DELETION MUTANT; DNA PROBE; DNA RNA HYBRIDIZATION; GENETIC TRANSFECTION; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROCESSING; RNA SEQUENCE; VIRUS ASSEMBLY; ANIMAL; BINDING SITE; CELL STRAIN COS1; GENETICS; HUMAN; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

ANIMALS; BASE SEQUENCE; BINDING SITES; COS CELLS; GENE PRODUCTS, GAG; GENE PRODUCTS, POL; HIV-1; HIV-1 REVERSE TRANSCRIPTASE; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN PRECURSORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RNA, TRANSFER, AMINO ACYL; VIRUS ASSEMBLY;

EID: 0031592385     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0742     Document Type: Article

References (46)

1. Babé, L. M., Rosé, U. R. & Craik, C. S. (1995). Trans-dominant inhibitory human immunodeficiency virus type 1 protease monomers prevent protease activation and viral maturation. Proc. Natl Acad. Sci. USA, 92, 10069-10073.
2. Baillon, J. G., Nashed, N. T., Kumar, A., Wilson, S. H. & Jerina, D. M. (1991). A leucine zipper-like motif may mediate HIV reverse transcriptase subunit binding. New Biolog. 3, 1015-1019.
3. Barat, C., Lullien, V., Schatz, O., Keith, G. & Darlix, J. L. (1989). HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA. EMBO J. 8, 3279-3285.
4. Beard, W. A., Stahl, S. J., Kim, H.-R., Bebenek, K., Kumar, A., Strub, M.-P., Becerra, S. P., Kunkel, T. A. & Wilson, S. H. (1994). Structure/function studies of human immunodeficiency virus type 1 reverse transcriptase. Alanine scanning mutagenesis of an alpha-helix in the thumb subdomain. J. Biol. Chem. 269, 28091-28097.
5. Bour, S., Boulerice, F. & Wainberg, M. A. (1991). Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1. J. Virol. 65, 6387-6396.
6. Trp as primer for RNA-directed DNA polymerase: structural determinants of function. J. Biol. Chem. 254, 1866-1874.
7. Divita, G., Restle, T., Goody, R. S., Chermann, J.-C. & Baillon, J. G. (1994). Inhibition of human immunodeficiency virus type 1 reverse transcriptase dimerization using synthetic peptides derived from the connection domain. J. Biol. Chem. 269, 13080-13083.
8. Erikson, E. & Erikson, R. L. (1971). Association of 4 S RNA with oncornavirus RNA. J. Virol. 8, 254-256.
9. Faras, A. J., Garapin, A. C., Levinson, W. E., Bishop, J. M. & Goodman, H. M. (1973). Characterization of the low-molecular-weight RNAs associated with the 70 S RNA of Rous sarcoma virus. J. Virol. 12, 334-342.
10. Franke, E. K., Yuan, H. E. H. & Luban, J. (1994). Specific incorporation of cyclophilin A into HIV-1 virions. Nature, 372, 359-362.
11. Gu, Z., Fletcher, R. S., Arts, E. J., Wainberg, M. A. & Parniak, M. A. (1994). The K65R mutant reverse transcriptase of HIV-1 cross-resistant to 2′,3′-dideoxycytidine, 2′,3′-dideoxy-3′-thiacytidine, and 2′,3′-dideoxyinosine shows reduced sensitivity to specific dideoxynucleoside triphosphate inhibitors in vitro. J. Biol. Chem. 269, 28118-28122.
12. Guo, J., Wu, W., Yuan, Z. Y., Post, K., Crouch, R. J. & Levin, J. (1995). Defects in primer-template binding, processive DNA synthesis, and RNase H activity associated with chimeric reverse transcriptase having the murine leukemia virus polymerase domain joined to Escherichia coli RNase H. Biochemistry, 34, 5018-5029.
13. Haseltine, W. A., Panet, A., Smoler, D., Baltimore, D., Peters, G., Harada, F. & Dahlberg, J. E. (1977). The interaction of tryptophan tRNA and avian myeloblastosis virus reverse transcriptase: further characterization of the binding reaction. Biochemistry, 16, 3625-3632.
14. Hermann, T., Meier, T., Gotte, M. & Heumann, H. (1994). The "Helix clamp" in HIV-1 reverse transcriptase: a new nucleic acid binding motif common in nucleic acid polymerases. Nucl. Acids Res. 22, 4625-4633.
15. Higuchi, R. (1990). Recombinant PCR. In PCR Protocols: A Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J., eds), pp. 177-183. Academic Press, Inc., New York.
16. Hottiger, M., Gramatikoff, K., Georgiev, O., Chaponnier, C., Schaffner, W. & Hubscher, U. (1995). The large sububit of HIV-1 reverse transcriptase interacts with β-actin. Nucl. Acids Res. 23, 736-741.
17. Jiang, M., Mak, J., Ladha, A., Cohen, E., Klein, M., Rovinski, B. & Kleiman, L. (1993). Identification of tRNAs incorporated into wild-type and mutant human immunodeficiency virus type 1. J. Virol. 67(6), 3246-3253.
18. Kalpana, G. V., Marmon, S., Wang, W., Crabtree, G. R. & Goff, S. P. (1994). Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. Science, 266, 2002-2006.
19. Kaplan, A. H., Manchester, M. & Swanstrom, R. (1994). The activity of the protease of human immunodeficiency virus type 1 is initiated at the membrane of infected cells before the release to occur with maximum efficiency. J. Virol. 68, 6782-6786.
20. Karczewski, M. K. & Strebel, K. (1996). Cytoskeleton association and virion incorporation of the human immunodeficiency virus type 1 Vif protein. J. Virol. 70, 494-507.
21. Kohlstaedt, L. A., Wang, J., Friedman, J. M., Rice, P. A. & Steitz, T. A. (1992). Crystal structure at 3.5 Angstrom resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science, 256, 1783-1790.
22. Leis, J., Aiyar, A. & Cobrinik, D. (1993). Regulation of initiation of reverse transcription of retroviruses. In Reverse Transcriptase (Skalka, A. M. & Goff, S. P., eds), vol. 1, pp. 33-47, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Pro during murine leukemia virus assembly. J. Virol. 38, 403-408.
24. Levin, J. G., Hu, S. C., Rein, A., Messer, L. I. & Gerwin, B. I. (1984). Murine leukemia virus mutant with a frameshift in the reverse transcriptase coding region: implications for pol gene structure. J. Virol. 51, 470-478.
25. Li, X., Amandoron, E., Wainberg, M. A. & Parniak, M. A. (1993). Generation and characterization of murine monoclonal antibodies reactive against N-terminal and other regions of HIV-1 reverse transcriptase. J. Med. Virol. 39, 251-250.
26. Lys into human immunodeficiency virus type 1 particles. J. Virol. 68, 2065-2072.
27. Lys3 to reverse transcriptase from human immunodeficiency virus type 1. EMBO J. 14(11), 2679-2687.
28. Panet, A. & Berliner, H. (1978). Binding of tRNA to reverse transcriptase of RNA tumor viruses. J. Virol. 26, 214-220.
29. gag and is incorporated into virus-like particles. J. Virol. 66, 6304-6313.
30. Peters, G. G. & Hu, J. (1980). Reverse transcriptase as the major determinant for selective packaging of tRNAs into avian sarcoma virus particles. J. Virol. 36, 692-700.
31. Peters, G., Harada, F., Dahlberg, J. E., Panet, A., Haseltine, W. A. & Baltimore, D. (1977). Low-molecular-weight RNAs of Moloney murine leukemia virus: identification of the primer for RNA-directed DNA synthesis. J. Virol. 21, 1031-1041.
32. Prasad, V. R. & Goff, S. P. (1989). Linker insertion mutagenesis of the human immunodeficiency virus reverse transcriptase expressed in bacteria: definition of the minimal polymerase domain. Proc. Natl Acad. Sci. USA, 86, 3104-3108.
33. Raba, M., Limburg, K., Burghagen, M., Katz, J., Simsek, M., Heckman, J., Rajbhandary, U. & Gross, H. (1979). Nucleotide sequence of three isoaccepting lysine tRNAs from rabbit liver and SV40-transformed mouse fibroblasts. Eur. J. Biochem. 97, 305-318.
34. Ratner, L., Haseltine, W. A., Patarca, R., Livak, K. J., Starcich, B., Josephs, S. F., Doran, E. R., Rafalski, J. A., Whitehorn, E. A., Baumeister, K. et al. (1985). Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature, 313, 277-284.
35. Restle, T., Pawlita, M., Sczakiel, G., Muller, B. & Goody, R. S. (1992). Structure-function relationships of HIV-1 reverse transcriptase determined using monoclonal antibodies. J. Biol. Chem. 267, 14654-14661.
36. Richman, D. D. (1993). Resistance of clinical isolates of human immunodeficiency virus to antiretroviral agents. Antimicrob. Agents Chemother. 37, 1207-1213.
37. Rosé, U. R., Babé, L. M. & Craik, C. S. (1995). Defining the level of human immunodeficiency virus type 1 (HIV-1) protease activity required for HIV-1 particle maturation and infectivity. J. Virol. 69, 2751-2758.
38. Rosenthal, L. J. & Zamecnik, P. C. (1973). Amino acid acceptor activity of the "70 S-associated" 4 S RNA from avian myeloblastosis virus. Proc. Natl Acad. Sci. USA, 70, 1184-1185.
39. Lys. Eur. J. Biochem. 184, 367-374.
40. Lys as evidenced by footprinting studies and inhibition with synthetic oligoribonucleotides. J. Mol. Biol. 226, 1-6.
41. Sawyer, R. C. & Dahlberg, J. E. (1973). Small RNAs of Rous sarcoma virus: characterization by two-dimensional polyacrylamide gel electrophoresis and fingerprint analysis. J. Virol. 12, 1226-1237.
42. gag-pol from human immunodeficiency virus type 1 into virus-like particles. J. Virol. 67, 2266-2275.
43. Thali, M., Bukovsky, A., Kondo, E., Rosenwirth, B., Walsh, C. T., Sodroski, J. & Göttlinger, H. G. (1994). Functional association of cyclophilin A with HIV-1 virions. Nature, 372, 363-365.
44. Wills, J. W. & Craven, R. C. (1991). Form, function, and use of retroviral gag proteins. AIDS, 5, 639-654.
45. Wu, J., Amandoron, E., Li, X., Wainberg, M. A. & Parniak, M. A. (1993). Monoclonal antibody-mediated inhibition of HIV-1 reverse transcriptase polymerase activity. J. Biol. Chem. 268, 9980-9985.
46. Zhao, L, J., Mukherjee, S. & Narayan, O. (1994). Biochemical mechanism of HIV-1 Vpr function. Specific interaction with a cellular protein. J. Biol. Chem. 269, 15577-15582.