Purification and characterization of ascorbate peroxidase in roots of Japanese radish

Plant Science

Volumn 125, Issue 2, 1997, Pages 137-145

  Ohya, Toshihide ^a   Morimura, Yoko ^b   Saji, Hikaru ^c   Mihara, Toshikazu ^d   Ikawa, Tomoyoshi ^d  

^a Akita Res Inst for Genetic Rsrc   (Japan)

^b Keisen Junior College   (Japan)

^c NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES   (Japan)

^d UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Ascorbate peroxidase; Ascorbic acid; Hydrogen peroxide; Japanese radish (Raphanus sativus); Roots

Indexed keywords

ASCORBIC ACID; CATECHOL; DIAMINOBENZIDINE; GUAIACOL; HEME; PEROXIDASE; THIOL GROUP;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; NONHUMAN; PLANT ROOT; SEQUENCE HOMOLOGY;

EID: 0031591586     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(97)00063-0     Document Type: Article

References (23)

1. [1] K. Asada, K. Kiso, K. Yoshikawa, Univalent reduction of molecular oxygen by spinach chloroplasts in illumination, J. Biol. Chem. 249 (1974) 2175-2181.
2. [2] K. Asada, M. Urano, M. Takahashi, Subcellular location of superoxide dismutase in spinach leaves and preparation and properties of crystalline spinach Ssperoxide dismutase, Eur. J. Biochem. 36 (1973) 257-266.
3. [3] Y. Nakano, K. Asada, Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts, Plant Cell Physiol. 22 (1981) 867-880.
4. [4] D.J. Gillham, A.D. Dodge, Hydrogen-peroxide-scavenging systems within pea chloroplasts, Planta 167 (1986) 246-251.
5. [5] G.-X. Chen, K. Asada, Ascorbate peroxidase in tea leaves. Occurence of two isozymes and the differences in their enzymatic and molecular properties, Plant Cell Physiol. 30 (1989) 987-998.
6. [6] D.A. Dalton, F.J. Hanus, S.A. Rusell, H.J. Evans, Purification, properties and distribution of ascorbate peroxidase in legume root nodules, Plant Physiol. 83 (1987) 789-794.
7. [7] R. Mittler, B.A. Zilinskas, Purification and characterization of pea cytosolic ascorbate peroxidase, Plant Physiol. 97 (1991) 962-968.
8. [8] T. Koshiba, Cytosolic ascorbate peroxidase in seedlings and leaves of maize, Plant Cell Physiol. 34 (1993) 713-721.
9. [9] R. Mittler, B.A. Zilinskas, Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase, J. Biol. Chem. 267 (1992) 21802-21807.
10. [10] A. Kubo, H. Saji, K. Tanaka, N. Kondo, Genomic DNA structure of a gene encoding cytosolic ascorbate peroxidase from Arabidopsis thaliana. FEBS Lett. 315 (1993) 313-317.
11. [11] Y. Morimura, T. Ohya, T. Ikawa, Presence of ascorbate-peroxidizing enzymes in roots of Brassica campestris L. cv. Komatsuna, Plant Sci. 117 (1996) 55-63.
12. [12] U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
13. [13] T. Ohya, K. Naito, H. Suzuki, Combined effect of benzyladenine and potassium on the level of light-harvesting chlorophyll a/b protein in detached cucumber cotyledons, Z. Pflanzenphysiol. 108 (1982) 39-47.
14. [14] S.M. Read, D.H. Northcote, Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein, Anal. Biochem. 116 (1981) 53-64.
15. [15] C. Miyake, W.-H. Cao, K. Asada, Purification and molecular properties of the thylakoid-bound ascorbate peroxidase in spinach chloroplasts, Plant Cell Physiol. 34 (1993) 881-889.
16. [16] H. Saji, K. Tanaka, N. Kondo, Monoclonal antibodies to spinach ascorbate peroxidase and immunochemical detection of the enzyme in eight different plant species, Plant Sci. 69 (1990) 1-9.
17. [17] Y. Nakano, K. Asada, Purification of ascorbate peroxidase in spinach chloroplasts. Its inactivation in ascorbate-depleted medium an reactivation by monodehydro-ascorbate radical, Plant Cell Physiol. 28 (1987) 131-140.
18. [18] G.-X. Chen, K. Asada, Hydroxyurea and p-aminophenol are the suicide inhibitors of ascorbate peroxidase, J. Biol. Chem. 265 (1990) 2775-2781.
19. [19] C. Miyake, K. Asada, Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoid, Plant Cell Physiol. 33 (1992) 541-553.
20. [20] K. Amako, G.-X. Chen, K. Asada, Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant Cell Physiol. 35 (1994) 497-504.
21. 2 decomposing system in chloroplasts, Plant Cell Physiol. 26 (1985) 1425-1431.
22. [22] N. Smirnoff, S.V. Colombe, Drought influences the activity of enzymes of the chloroplast hydrogen peroxide scavenging system, J. Exp. Bot. 39 (1988) 1097-1108.
23. [23] Y. Morita, C. Yoshida, I. Kitamura, S. Ida, Isoenzymes of Japanese-radish peroxidase, Agric. Biol. Chem. 34 (1970) 1191-1197.