Purification and characterization of 20 S proteasome from wheat leaves

Plant Science

Volumn 125, Issue 2, 1997, Pages 127-136

  Fernández Murray, Pedro ^a   Giordano, Carla V ^a   Passeron, Susana ^a   Barneix, Atilio J ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

20 S proteasome; Multicatalytic proteinase; Proteolysis; Wheat leaves

Indexed keywords

ALDEHYDE; CHLOROMERCURIBENZENESULFONIC ACID; CHYMOTRYPSIN; DODECYL SULFATE SODIUM; POLYLYSINE; POLYPEPTIDE; PROTEASOME; PROTEINASE; TRYPSIN;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; NONHUMAN; PLANT LEAF; WHEAT;

EID: 0031591584     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(97)00060-5     Document Type: Article

References (42)

1. [1] O. Coux, K. Tanaka and A.L. Goldberg, Structure and functions of the 20 S and 26 S proteasomes. Ann. Rev. Biochem., 65 (1996) 801-847.
2. [2] J. Löwe, D. Stock, R. Jap, P. Zwickl, W. Baumeister and R. Huber. Crystal structure of the 20 S proteasome from the archaeon T-acidophilum at 3.4 Å resolution. Science, 268 (1995) 533-539.
3. [3] E. Seemüller, A. Lupas, D. Stock, J. Löwe, R. Huber and W. Baumeister, Proteasome from Thermoplasma acidophilum: A threonine protease. Science, 268 (1995) 579-582.
4. [4] R.L. Stein, F. Melandri and L. Dick, Kinetic characterization of the chymotryptic activity of the 20 S proteasome. Biochemistry, 35 (1996) 3899-3908.
5. [5] M. Hochstrasser, Ubiquitin, proteasomes and the regulation of intracellular protein degradation. Curr. Opin. Cell Biol., 7 (1995) 215-223.
6. [6] W. Heinemeyer, N. Tröndle, G. Albrecht and D.H. Wolf, PRE5 and PRE6, the last missing genes encoding 20 S proteasome subunits from yeast? Indication for a set of 14 different subunits in the eukaryotic proteasome core. Biochemistry, 33 (1994) 12229-12237.
7. [7] J.M. Peters, Proteasomes: Protein degradation machines of the cell. Trends Biochem. Sci., 19 (1994) 377-382.
8. [8] S. Jentsch, The ubiquitin-conjugation system. Annu. Rev. Genet., 26 (1992) 179-207.
9. [9] A. Ciechanover, The ubiquitin-proteasome proteolytic pathway. Cell, 79 (1994) 13-21.
10. [10] H.L. Pahl and P.A. Baeuerle, Control of gene expression by proteolysis. Curr. Opin. Cell Biol., 8 (1996) 340-347.
11. [11] A. Kremp, M. Schliephacke, U. Kull and H.P. Schmid, Prosomes exist in plant cells too. Exp. Cell Res., 166 (1986) 553-557.
12. [12] A. P. Arrigo, M. Simon, J.L. Darlix and P.F. Spahr, A 20 S particle ubiquitous from yeast to human. J. Mol. Evol., 25 (1987) 141-150.
13. [13] M. Schliephacke, A. Kremp, H.P. Schmid, K. Köhler and U. Kull, Prosomes (proteasomes) of higher plants. Eur. J. Cell Biol., 55 (1991) 114-121.
14. [14] M. Ozaki, K. Fujinami, K. Tanaka, Y. Amemiya, T. Sato, N. Ogura and H. Nakagawa, Purification and initial characterization of the proteasome from the higher plant Spinacia oleracea. J. Biol. Chem., 267 (1992) 21678-21684.
15. [15] B. Skoda and L. Malek, Dry pea seed proteasome. Purification and enzymic activities. Plant Physiol., 99 (1992) 1515-1519.
16. [16] P. Genschik, G. Philipps, C. Gigot and J. Fleck, Cloning and sequence analysis of a cDNA clone from Arabidopsis thaliana homologous to a proteasome α subunit from Drosophila. FEBS Lett., 309 (1992) 311-315.
17. [17] B.W. Shirley and H.M. Goodman, An Arabidopsis gene homologous to mammalian and insect genes encoding the largest proteasome subunit. Mol. Gen. Genet., 241 (1993) 586-594.
18. [18] P. Genschik, E. Jamet, G. Philipps, Y. Parmentier, C. Gigot and J. Fleck, Molecular characterization of a β-type proteasome subunit from Arabidopsis thaliana co-expressed at a high level wilh an α-type proteasome subunit early in the cell cycle. Plant J., 6 (1994) 537-546.
19. [19] D.R. Hoagland and D.I. Arnon, The water culture method for growing plants without soil. Calif. Agric. Exp. Stn. Circ., (1950) 347.
20. [20] A.C. Bratton and E.K. Marshall, A new coupling component for sulfanilamide determination. J. Biol. Chem., 128 (1939) 537-550.
21. [21] J.A. Goldbarg and A.M. Rutemburg, The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases. Cancer, 11 (1958) 283-291.
22. [22] P. Andrews, The gel filtration behaviour of proteins related to their molecular weights over a wide range. Biochem. J., 96 (1965) 595-606.
23. [23] B.J. Davis, Disc electrophoresis II. Method and application to human serum proteins. Ann. New York Acad. Sci., 121 (1964) 404-427.
24. [24] U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227 (1970) 680-685.
25. [25] P.Z. O'Farrell, H.M. Goodman and P.H. O'Farrell, High resolution two-dimensional electrophoresis of basic and well as acidic proteins. Cell, 12 (1977) 1133-1142.
26. [26] B.S. Dumbar, Two-dimensional electrophoresis and immunological techniques. Plenum Press, New York, 1987, pp. 293-298.
27. [27] H. Towbin, T. Staehelin and J. Gordon, Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and applications. Proc. Natl. Acad. Sci. USA, 76 (1979) 4350-4354.
28. [28] M.M. Bradford, A rapid and sensitive method for the quantitation of micrograms quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem., 72 (1976) 248-254.
29. [29] K. Fujinami, N. Tanahashi, K. Tanaka, A. Ichihara, Z. Cejka, W. Baumeister, M. Miyawaki, T. Sato and H. Nakagawa, Purification and characterization of the 26 S proteasome from spinach leaves. J. Biol. Chem., 269 (1994) 25905-25910.
30. [30] J.G. Castaño, E. Mahillo, P. Aritzi and J. Arribas, Phosphorylation of C8 and C9 subunits of the multicatalytic proteinase by casein kinase II and identification of the C8 phosphorylation sites by direct mutagenesis. Biochemistry, 35 (1996) 3782-3789.
31. [31] G.G.F. Mason, K.B. Hendil and A.J. Rivett, Phosphorylation of proteasomes in mammalian cells. Identification of two phosphorylated subunits and the effect of phosphorylation on activity. Eur. J. Biochem., 238 (1996) 453-462.
32. [32] K. Tanaka, Molecular biology of proteasomes. Mol. Biol. Reports, 21 (1995) 21-26.
33. [33] A.J. Koster, J. Walz, A. Lupas and W. Baumeister, Structural features of archaebacterial and eukaryotic proteasomes. Mol. Biol. Reports, 21 (1995) 11-20.
34. [34] A.J. Rivett, P.J. Savory and H. Djaballah, Multicatalytic endopeptidase complex: proteasome. Meth. Enzymol., 244 (1994) 331-350.
35. [35] R. Vierstra, Protein degradation in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol., 44 (1993) 385-410.
36. [36] P.M. Hatfield and R.D. Vierstra, Ubiquitin-dependent proteolytic pathway in wheat germ: Isolation of multiple forms of ubiquitin-activating enzyme, El. Biochemistry, 28 (1989)735-742.
37. [37] S. Szarka, M. Fitch, S. Schaerer and M. Moloney, Classification and expression of a family of cyclin gene homologies in Brassica napus. Plant. Mol. Biol., 27 (1995) 263-275.
38. [38] J. von Kampen, M. Wettern and M. Schulz, The ubiquitin system in plants. Physiol. Plant., 97 (1996) 618-624.
39. [39] W.R. Belknap and J.E. Garbarino, The role of ubiquitin in plant senescence and stress responses. Trends Plant Sci., 1 (1996) 331-335.
40. [40] J. Shanklin, M. Jabben and R.D. Vierstra, Red light-induced formation of ubiquitin-phytochrome conjugate: Identification of possible intermediates of phytochrome degradation. Proc. Natl. Acad. Sci. USA, 84 (1987) 359-363.
41. [41] S.E. Courtney, C.C. Rider and A.D. Stead, Changes in protein ubiquitination and the expression of ubiquitin-encoding transcripts in day lily petals during floral development and senescence. Physiol. Plant., 91 (1994) 196-204.
42. [42] M.L. Pinedo, S.M. Goicoechea, L. Lamattina and R.D. Conde, Estimation of ubiquitin and ubiquitin mRNA content in dark senescing wheat leaves. Biol. Plant., 38 (1996) 321-328.