Improvement of the 3′-5′ Exonuclease Activity of Taq DNA Polymerase by Protein Engineering in the Active Site

Molecules and Cells

Volumn 7, Issue 3, 1997, Pages 419-424

  Park, Yonghyun ^a   Choi, Hyeja ^a   Lee, Dae Sil ^b   Kim, Youngsoo ^a  

^a Yeungnam University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED DNA POLYMERASE; DNA DIRECTED DNA POLYMERASE BETA; EXODEOXYRIBONUCLEASE; EXODEOXYRIBONUCLEASE V; TAQ POLYMERASE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; THERMUS;

AMINO ACID SEQUENCE; BINDING SITES; DNA POLYMERASE I; DNA-DIRECTED DNA POLYMERASE; ESCHERICHIA COLI; EXODEOXYRIBONUCLEASE V; EXODEOXYRIBONUCLEASES; GENE EXPRESSION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY, AMINO ACID; TAQ POLYMERASE; THERMUS;

EID: 0031591507     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Barnes, W. M. (1992) Gene 112, 29-35.
2. Beese, L. S., and Steitz, T. A. (1991) EMBO J. 10, 25-33.
3. Beese, L. S., Derbyshire, V., and Steitz, T. A. (1993) Science 260, 352-355.
4. Ceska, T. A., Sayers, J. R., Stier, G., and Suck, D. (1996) Nature 382, 90-94.
5. Davies, J. F., Hostomska, Z., Homstomsky, Z., Jordan, S. R., and Mattews, D. A. (1991) Science 252, 88-95.
6. Davies, J. F., Almassey, R. J., Hostomska, Z., Ferre, R. A., and Hostomsky, Z. (1994) Cell 76, 1123-1133.
7. Delarue, M., Poch, O., Tordo, N., Moras, D., and Argos, P. (1990) Prot. Eng. 3, 461-467.
8. Derbyshire, V., Freemont, P. S., Sanderson, M. R., Beese, L., Friedman, J. M., Joyce, C. M., and Steitz, T. A. (1988) Science 240, 199-201.
9. Derbyshire, V., Grindley, N., and Joyce, C. M. (1991) EMBO J. 10, 17-24.
10. Derbyshire, V., Pinsonneault, J. K., and Joyce, C. M. (1995) Methods Enzymol. 262, 363-385.
11. Eckert, K. A., and Kunkel, T. A. (1990) Nucleic Acids Res. 18, 3739-3744.
12. Engelke, D. R., Krikos, A., Bruck, M. E., and Ginsberg, D. (1990) Anal. Biochem. 191, 396-400.
13. Esteban, J. A., Soengas, M. S., Salas, M., and Blanco, L. (1994) J. Biol. Chem. 269, 31946-31954.
14. Flaman, J.-M., Frebourg, T., Moreau, V., Charbonnier, F., Martin, C., Ishioka, C., Friend, S. H., and Iggo, R. (1994) Nucleic Acids Res. 22, 3259-3260.
15. Freemont, P. S., Ollis, D. L., Steitz, T. A., and Joyce, C. M. (1986) Proteins 1, 66-73.
16. Holland, P. M., Abramson, R. D., Watson, R., and Gelfand, D. H. (1991) Proc. Natl. Acad. Sci. USA 88, 7276-7280.
17. Innis, M. A., Myambo, K. B., Gelfand, D. H., and Brow, M. A. D. (1988) Proc. Natl. Acad. Sci. USA 85, 9436-9440.
18. Joyce, C. M., and Steitz, T. A. (1994) Annu. Rev. Biochem. 63, 777-822.
19. Kankare, J., Neal, G. S., Salminen, T., Glumhoff, T., Cooperman, B. S., Rahti, R., and Goldman, A. (1994) Prot. Eng. 7, 823-830.
20. Kim, E., and Wyckoff, H. H. (1991) J. Mol. Biol. 218, 449-464.
21. Kim, Y. (1995) Mol. Cells 5, 452-460.
22. Kim, Y., Eom, S. H., Wang, J., Lee, D. S., Suh, S. W., and Steitz, T. A. (1995) Nature 376, 612-616.
23. Kornberg, A., and Baker, T. (1992) DNA Replication, 2nd Ed, Freeman and Company, San Francisco, CA.
24. Kwon, S. T., Kim, J. S., Park, J. H., Koh, S., and Lee, D. S. (1991) Mol. Cells 1, 369-375.
25. Lawyer, F. C., Stoffel, S., Saiki, R. K., Myambo, K., Drummond, R., and Gelfand, D. H. (1989) J. Biol. Chem. 264 6427-6437.
26. Longley, M. J., Bennett, S. E., and Mosbaugh, D. W. (1990) Nucleic Acids Res. 18, 7317-7322.
27. Lyamichev, V., Brow, M. A. D., and Dahlberg, S. E. (1993) Science 260, 778-7835.
28. Myers, T. W., and Gelfand, D. H. (1991) Biochemistry 30, 7661-7665.
29. Ollis, D. L., Brick, P., Hamlin, R., Xuong, N. G., and Steitz, T. A. (1985) Nature 313, 762-766.
30. Park, J. H., Kim, J. S., Kwon, S. T., and Lee, D. S. (1993) Eur. J. Biochem. 214, 135-140.
31. Pelletier, H., Sawaya, M. R., Kumar, A., Wilson, S. H., and Kraut, J. (1994) Science 264, 1891-1903.
32. Reha-Krantz, L. J. (1992) Gene 112, 133-137.
33. Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erich, H. A., and Arnheim, N. (1985) Science 230, 1350-1354.
34. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1988) Science 239, 487-491.
35. Yang, W., Hendrickson, W. A., Crouch, R. J., and Satow, Y. (1990) Science 252, 88-95.