Mutant RecA proteins which form hexamer-sized oligomers

Journal of Molecular Biology

Volumn 266, Issue 2, 1997, Pages 306-316

  Logan, Karen M ^a   Skiba, Mark C ^a   Eldin, Sherif ^a   Knight, Kendall L ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Homologous recombination; Oligomer mutants; Oligomeric structure; Protein filament; RecA protein

Indexed keywords

MUTANT PROTEIN; OLIGOMER; PROTEIN SUBUNIT; RECA PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; DNA RECOMBINATION; DNA REPAIR; PRIORITY JOURNAL;

EID: 0031581870     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0751     Document Type: Article

References (34)

1. Benight A. S., Wilson D. H., Budzynski D. M., Goldstein R. F. Dynamic light scattering investigations of RecA self assembly and interactions with single strand DNA. Biochimie. 73:1991;143-155.
2. Brenner S. L., Zlotnik A., Griffith J. D. RecA protein self-assembly. Multiple discrete aggregation states. J. Mol. Biol. 204:1988;959-972.
3. Bryant F. R., Taylor A. R., Lehman I. R. Interaction of the recA protein of Escherichia coli with single-stranded DNA. J. Biol. Chem. 260:1985;1196-1202.
4. Budzynski D. M., Gao X., Benight A. S. Isolation, characterization, and magnesium-induced self-association kinetics of discrete aggregates of RecA protein fromEscherichia coli. Biopolymers. 38:1996;471-491.
5. Chow S. A., Honigberg S. M., Bainton R. J., Radding C. M. Patterns of nuclease protection during strand exchange: recA protein forms heteroduplex DNA by binding to strands of the same polarity. J. Biol. Chem. 261:1986;6961-6971.
6. Craig N. L., Roberts J. W. E. coli recA protein-directed cleavage of phage λ repressor requires polynucleotide. Nature. 283:1980;26-30.
7. DiCapua E., Engel A., Stasiak A., Koller Th. Characterization of complexes between recA protein and duplex RNA by electron microscopy. J. Mol. Biol. 157:1982;87-103.
8. Dutreix M., Burnett B., Bailone A., Radding C. M., Devoret R. A partially deficient mutant, recA1730, that fails to form normal nucleoprotein filaments. Mol. Gen. Genet. 232:1992;489-497.
9. Egelman E. H. What do X-ray crystallographic and electron microscopic structural studies of the RecA protein tell us about recombination? Curr. Opin. Struct. Biol. 3:1993;189-197.
10. Egelman E. H., Stasiak A. Structure of recA-DNA complexes: II. Local conformational changes visualized in bundles of recA-ATP-γ-S filaments. J. Mol. Biol. 200:1988;329-349.
11. Heuser J., Griffith J. Visualization of RecA protein and its complexes with DNA by quick-freeze/deep-etch electron microscopy. J. Mol. Biol. 210:1989;473-484.
12. Hewat E. A., Ruigrok R. W. H., DiCapua E. Activation of recA protein: the pitch of the helical complex with single-stranded DNA. EMBO J. 10:1991;2695-2698.
13. Kowalczykowski S. C., Dixon D. A., Eggleston A. K., Lauder S. D., Rehrauer W. M. Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58:1994;401-465.
14. Lee J. W., Cox M. M. Inhibition of RecA protein promoted ATP hydrolysis. I. ATPγS and ADP are antagonistic inhibitors. Biochemistry. 29:1990a;7666-7676.
15. Lee J. W., Cox M. M. Inhibition of RecA protein promoted ATP hydrolysis. II. Longitudinal assembly and disassembly of RecA protein filaments mediated by ATP and ADP. Biochemistry. 29:1990b;7677-7683.
16. Little J. W. LexA cleavage and other self-processing reactions. J. Bacteriol. 175:1993;4943-4950.
17. Little J. W., Mount D. W. The SOS regulatory system of Escherichia coli. Cell. 29:1982;11-22.
18. Little J. W., Edmiston S. H., Pacelli L. Z., Mount D. W. Cleavage of the Escherichia coli lexA protein by the recA protease. Proc. Natl. Acad. Sci. USA. 77:1980;3225-3229.
19. McEntee K, Weinstock G. M., Lehman I. R. Binding of the recA protein of Escherichia coli to single- and double-stranded DNA. J. Biol. Chem. 256:1981;8835-8844.
20. Morrical S. W., Cox M. M. Light scattering studies of the recA protein of Escherichia coli relationship between free recA filaments and the recA•ssDNA complex. Biochemistry. 24:1985;760-767.
21. Nastri H. G., Knight K. L. Identification of residues in the L1 region of the RecA protein which are important to recombination and coprotease activities. J. Biol. Chem. 269:1994;26311-26322.
22. Nguyen T. T., Muench K. A., Bryant F. R. Inactivation of the recA protein by mutation of histidine 97 or lysine 248 at the subunit interface. J. Biol. Chem. 268:1993;3107-3113.
23. Petsko G. A. Not just your average structures. Nature Struct. Biol. 3:1996;565-566.
24. Radding C. M. Helical RecA nucleoprotein filaments mediate homologous pairing and strand exchange. Biochem. Biophys Acta. 1008:1989;131-145.
25. Richards F. M. The interpretation of protein structures: total volume, group volume distributions and packing density. J. Mol. Biol. 82:1974;1-14.
26. Roca A. I., Cox M. M. The RecA protein: structure and function. Crit. Rev. Biochem. Mol. Biol. 25:1990;415-456.
27. Ruigrok R. W. H., DiCapua E. On the polymerization state of recA in the absence of DNA. Biochimie. 73:1991;191-197.
28. Skiba M. C., Knight K. L. Functionally important residues at a subunit interface site in the RecA protein from Escherichia coli. J. Biol. Chem. 269:1994;3823-3828.
29. Story R. M., Weber I. T., Steitz T. A. The structure of the E. coli recA protein monomer and polymer. Nature. 355:1992;318-325.
30. West S. C. Enzymes and molecular mechanisms of genetic recombination. Annu. Rev. Biochem. 61:1992;603-640.
31. Wilson D. H., Benight A. S. Kinetic analysis of the pre-equilibrium steps in the self-assembly of RecA protein from Escherichia coli. J. Biol. Chem. 265:1990;7351-7359.
32. Yu X., Egelman E. H. Structural data suggest that the active and inactive forms of the RecA filament are not simply interconvertible. J. Mol. Biol. 227:1992;334-346.
33. Yu X., Angov E., Camerini-Otero R. D., Egelman E. H. Structural polymorphism of the RecA protein from the thermophilic bacterium Thermus aquaticus. Biophys. J. 69:1995;2728-2738.
34. Zagursky R. J., Berman M. L. Cloning vectors that yield high levels of single-stranded DNA for rapid sequencing. Gene. 27:1984;183-191.