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Volumn 7, Issue 10, 1997, Pages 1275-1278

Duplex- and triplex-forming properties of 4'-thio-modified oligodeoxynucleotides

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; OLIGODEOXYNUCLEOTIDE;

ARTICLE; DNA BINDING; IN VITRO STUDY; RNA BINDING; THERMAL ANALYSIS;

EID: 0031579954 PISSN: 0960894X EISSN: None Source Type: Journal
DOI: 10.1016/S0960-894X(97)00208-4 Document Type: Article

Times cited : (12)

References (15)

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8
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- See reference 9
- Melting temperature measurements were obtained under conditions previously published. See reference 9.
- Melting Temperature Measurements were Obtained under Conditions Previously Published

13
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15
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- The triplex melting temperature experiments were conducted by adding the 4′-thio-modified oligodeoxynucleotide (i.e. 2-6) to the pre-formed (intermolecular) target duplex (17) to form the triplex, which is composed of two strands of natural DNA plus the modified homopyrimidine strand. Salt conditions were 1 mM Mg2+, 140 mM K+, 10 mM Na+ in 10 mM cacodylate buffer, pH 7.7 (the counter ion was Cl-). The solutions contained 2 μM of both target duplex and modified oligonucleotide, giving a total strand concentration of 6 μM, and they were equilibrated for 60 minutes at room temperature prior to heating. The Tm of the control triplex (1/1/7) was measured in parallel with each of the modified triplexes
- m of the control triplex (1/1/7) was measured in parallel with each of the modified triplexes.

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