A chromogenic substrate for the continuous assay of mammalian phosphoinositide-specific phospholipase C

Bioorganic and Medicinal Chemistry Letters

Volumn 7, Issue 10, 1997, Pages 1239-1242

  Rukavishnikov, Aleksey V ^a   Ryan, Margret ^a   Griffith, O Hayes ^a   Keana, John F W ^a  

^a UNIVERSITY OF OREGON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOGENIC SUBSTRATE; INOSITOL DERIVATIVE;

ARTICLE; CHIRALITY; DRUG STRUCTURE; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME ASSAY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SPECTROPHOTOMETRY; TECHNIQUE;

EID: 0031579942     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(97)00198-4     Document Type: Article

References (23)

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3. (a) Noh, D.-Y.; Shin, S.H.; Rhee, S.G. Biochim. Biophys. Acta 1995, 1242, 99.
4. (b) Shimohama, S.; Perry, G.; Richey, P.; Praprotnik, D.; Takenawa, T.; Fukami K.; Whitehouse, P.J.; Kimura, J. Brain Res. 1995, 669, 217.
5. Essen L.-O., Perisic O., Cheung R., Katan M., Williams R.L. Nature. 380:1996;595.
6. (a) Rhee, S.G.; Ryu, S.H.; Lee, K.Y.; Cho, K.S. Meth. Enzymol. 1991, 197, 502.
7. (b) Bruzik, K.S.; Tsai, M.-D. Bioorg. Med. Chem. 1994, 2, 49.
8. Bruzik K.S., Tsai M.-W. J. Am. Chem. Soc. 114:1992;6361.
9. 1 2 = 8.0 Hz); 4.27 (q, 1H, J = 9.3 Hz); 7.34 (d, 2H, J = 9.0 Hz); 8.21 (d, 2H, J = 9.0 Hz).
10. Satisfactory analytical data (±0.4% of calcd values) were obtained for 2 (C H), mp 114-115 °C (hexane); 3 (C H), mp 111-113 °C (hexane); 5 (C H), mp 138-139 °C (ether-chloroform); 6 (C H), mp 98 °C (ethyl acetate); 13 (C H N), white powder, dec > 120 °C.
11. Bruzik K.S., Myers J. Tetrahedron Lett. 33:1992;1009.
12. Nagai H., Fujiwara T., Fujii M., Sekine M., Hata T. Nucl. Ac. Res. 17:1989;8581.
13. 399, background).
14. 1 was a generous gift from Dr. Mario J. Rebecchi, Health Sciences Center, State University of New York, Stony Brook, NY.
15. 2 represents the true substrate concentration.
16. 2 represents the true substrate concentration.
17. 2 represents the true substrate concentration.
18. 2 represents the true substrate concentration.
19. 2+, 0.1 mM dithiothreitol (Rebecchi, M.J.; Rosen, O.M. J. Biol. Chem. 1987, 262, 12526).
20. 2+, 0.1 mM dithiothreitol (Rebecchi, M.J.; Rosen, O.M. J. Biol. Chem. 1987, 262, 12526).
21. 2+, 0.1 mM dithiothreitol (Rebecchi, M.J.; Rosen, O.M. J. Biol. Chem. 1987, 262, 12526).
22. 1 is ≥10μM: Hepler, J.R.; Kozasa, T.; Smrcka, A.V.; Simon, M.I.; Rhee, S.G.; Sternweis, P.C.; Gilman, A.G. J. Biol. Chem. 1993, 268, 14367 and Cifuentes, M.E.; Honkanen, L.; Rebecchi, M.J. J. Biol. Chem. 1993, 268, 11586.
23. 1 is ≥10μM: Hepler, J.R.; Kozasa, T.; Smrcka, A.V.; Simon, M.I.; Rhee, S.G.; Sternweis, P.C.; Gilman, A.G. J. Biol. Chem. 1993, 268, 14367 and Cifuentes, M.E.; Honkanen, L.; Rebecchi, M.J. J. Biol. Chem. 1993, 268, 11586.