Minilibraries constructed from cDNA generated by arbitrarily primed RT-PCR: An alternative to normalized libraries for the generation of ESTs from nanogram quantities of mRNA

Gene

Volumn 186, Issue 1, 1997, Pages 135-142

  Dias Neto, Emmanuel ^a,b   Harrop, Richard ^c   Correa Oliveira, Rodrigo ^a   Wilson, R Alan ^c   Pena, Sergio D J ^b   Simpson, Andrew J G ^a,d  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^c UNIVERSITY OF YORK   (United Kingdom)

^d LUDWIG INSTITUTE FOR CANCER RESEARCH   (Brazil)

Author keywords

Automated DNA sequencing; Gene discovery; Low stringency RT PCR; Parasites; Reduced redundancy; Schistosomes

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA;

ARTICLE; DNA LIBRARY; DNA SEQUENCE; METHODOLOGY; MICROORGANISM; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHISTOSOMA; TUMOR BIOPSY;

ANIMALS; DATABASES, FACTUAL; DNA PRIMERS; DNA, COMPLEMENTARY; GENE LIBRARY; MICE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA-DIRECTED DNA POLYMERASE; SCHISTOSOMA MANSONI; SEQUENCE HOMOLOGY, NUCLEIC ACID; SEQUENCE TAGGED SITES;

SCHISTOSOMA;

EID: 0031579477     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00699-3     Document Type: Article

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