Cloning and expression of the major porin protein gene opcP of Burkholderia (formerly Pseudomonas) cepacia in Escherichia coli

Gene

Volumn 186, Issue 1, 1997, Pages 113-118

  Tsujimoto, Hideto ^a   Gotoh, Naomasa ^a   Yamagishi, Jun ichi ^b   Oyamada, Yoshihiro ^b   Nishino, Takeshi ^a  

^a KYOTO PHARMACEUTICAL UNIVERSITY   (Japan)

^b DAINIPPON SUMITOMO PHARMA CO LTD   (Japan)

Author keywords

Homology; Hydropathy plot; trc promoter

Indexed keywords

DNA FRAGMENT; ISOPROPYL THIOGALACTOSIDE; OLIGOMER; OUTER MEMBRANE PROTEIN; PORIN; SIGNAL PEPTIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; BURKHOLDERIA; ESCHERICHIA COLI; IMMUNOBLOTTING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PSEUDOMONAS;

AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BASE SEQUENCE; BURKHOLDERIA CEPACIA; CLONING, MOLECULAR; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

BURKHOLDERIA; BURKHOLDERIA CEPACIA; ESCHERICHIA COLI; MURINAE; PSEUDOMONAS;

EID: 0031579470     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00692-0     Document Type: Article

References (23)

1. Amann, E., Ochs, B. and Abel, K.J. (1988) Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 69, 301-315.
2. Benz, R. (1988) Structure and function of porins from Gram-negative bacteria. Annu. Rev. Microbiol. 42, 359-393.
3. Bernardi, A. and Bernardi, F. (1984) Complete sequence of pSC101. Nucleic Acids Res. 12, 9415-9426.
4. Burns, J. and Clark, D.K. (1992) Salicilate-inducible antibiotic resistance in Pseudomonas cepacia associated with absence of a pore-forming outer membrane protein. Antimicrob. Agents Chemother. 36, 2280-2285.
5. Cowan, S.W., Schirmer, T., Rummel, G., Steiert, M., Ghosh, R., Pauptit, R.A., Jansonius, J.N. and Rosenbusch, J.P. (1992) Crystal structures explain functional properties of two E. coli porins. Nature 358, 727-733.
6. Ederer, G.M. and Matsen, J.M. (1972) Colonization and infection with Pseudomonas cepacia. J. Infect. Dis. 125, 613-618.
7. Fass, R.J. and Barnishan, J. (1980) In vitro susceptibilities of nonfermentative Gram-negative bacilli other than Pseudomonas aeruginosa to 32 antimicrobial agents. Rev. Infect. Dis. 2, 841-853.
8. Gilligan, P.H. (1991) Microbiology of airway disease in patients with cystic fibrosis. Clin. Microbiol. Rev. 4, 35-51.
9. Gotoh, N., Itoh, N., Tsujimoto, H., Yamagishi, J., Oyamada, Y. and Nishino, T. (1994a) Isolation of OprM-deficient mutants of Pseudomonas aeruginosa by transposon insertion mutagenesis: evidence of involvement in multiple antibiotic resistance. FEMS Microbiol. Lett. 122, 267-274.
10. Gotoh, N., Nagino, K., Wada, K., Tsujimoto, H. and Nishino, T. (1994b) Burkholderia (formerly Pseudomonas) cepacia porin is an oligomer composed of two component proteins. Microbiology 140, 3285-3291.
11. Hancock, R.E.W. (1987) Role of porins in outer membrane permeability. J. Bacteriol. 169, 929-933.
12. Jeanteur, D., Lakey, J.H. and Pattus, F. (1991) The bacterial porin superfamily: sequence alignment and structure prediction. Mol. Microbiol. 5, 2153-2164.
13. Kyte, J. and Doolittle, R.F. (1982) A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105-132.
14. Li, Z.M., Hannah, J.H., Stibitz, S., Nguyen, N.Y., Manclark, C.R. and Brennan, M.J. (1991) Cloning and sequencing of the structural gene for the porin protein of Bordetella pertussis. Mol. Microbiol. 5, 1649-1656.
15. Nakae, T. Outer membrane of Salmonella. (1976) Isolation of protein complex that produces transmembrane channels. J. Biol. Chem. 251, 2176-2178.
16. Nakae, T. (1986) Outer-membrane permeability of bacteria. Crit. Rev. Microbiol. 13, 1-62.
17. Nikaido, H. (1989) Outer membrane barrier as a mechanism of antimicrobial resistance. Antimicrob. Agents Chemother. 33, 1831-1836.
18. Nikaido, H. and Rosenberg, E.Y. (1983) Porin channels in Escherichia coli: studies with liposomes reconstituted from purified proteins. J. Bacteriol. 153, 241-252.
19. Nikaido, H. and Vaara, M. (1985) Molecular basis of bacterial outer membrane permeability. Microbiol. Rev. 49, 1-32.
20. Parr, T.R., Moore, R.A., Moore, L.V. and Hancock, R.E.W. (1987) Role of porins in intrinsic antibiotic resistance of Pseudomonas cepacia. Antimicrob. Agents Chemother. 31, 121-123.
21. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
22. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.
23. Yoshimura, F. and Nikaido, H. (1985) The porin channels of Escherichia coli K-12. Antimicrob. Agents Chemother. 27, 84-92.