Use of organic cosmotropic solutes to crystallize flexible proteins: Application to T7 RNA polymerase and its complex with the inhibitor T7 lysozyme

Journal of Molecular Biology

Volumn 274, Issue 5, 1997, Pages 748-756

  Jeruzalmi, David ^a   Steitz, Thomas A ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

Cosmotropic solutes; Osmolytes; Protein crystallization; T7 lysozyme; T7 RNA polymerase

Indexed keywords

ALCOHOL DERIVATIVE; AMINO ACID; COSMOTROPIC AGENT; LYSOZYME; RNA POLYMERASE; SUGAR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIOPHAGE T7; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; DIFFRACTION; ENZYME PURIFICATION; IONIC STRENGTH; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; PROTEIN STRUCTURE; SYNCHROTRON; TECHNIQUE; X RAY CRYSTALLOGRAPHY;

EID: 0031578707     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.1366     Document Type: Article

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