Selective coupling of a highly basic peptide to an oligonucleotide

Tetrahedron Letters

Volumn 38, Issue 7, 1997, Pages 1183-1186

  Vivès, Eric ^a   Lebleu, Bernard ^a  

^a INSTITUT DE GÉNÉTIQUE MOLÉCULAIRE DE MONTPELLIER   (France)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE; PEPTIDE DERIVATIVE;

ARTICLE; CONJUGATION; DNA PROTEIN COMPLEX; DRUG PURIFICATION; DRUG SYNTHESIS; IN VITRO STUDY; METHODOLOGY; REACTION ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 0031575639     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(97)00059-2     Document Type: Article

References (16)

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14. 4Ac pH7.2) with dual absorbance detection at 254 nm and 550 nm. The yield of the labelling reaction was estimated to be 75% according to HPLC peak integration. The purified labelled ODN fractions were pooled and concentrated by partial evaporation on a Speed-Vacuum microcentrifuge before the coupling reaction with the peptide.
15. 8. The peptide was purified by semi-preparative C18 reversed phase HPLC. Analytical HPLC was carried out on Hypersyl C18-5mm column (4.6×250). Peptide molecular weight was determined by Electrospray Ionization Mass Spectrometry (calculated: 1996.3 Da; found: 1996.5). Composition and quantification of the peptide were determined after hydrolysis of an aliquot for 24h at 110°C. Peptide was resuspended in PBS (pH 7.3) at a concentration of 10mg/ml and kept frozen until further use.
16. 16. Ellmann, G.L. Arch. Biochem. Biophys. 1959, 82, 70-77.