A dye-based lymphocyte proliferation assay that permits multiple immunological analyses: mRNA, cytogenetic, apoptosis, and immunophenotyping studies

Journal of Immunological Methods

Volumn 210, Issue 1, 1997, Pages 25-39

  Zhi Jun, Y ^a   Sriranganathan, N ^a   Vaught, T ^a   Arastu, S K ^a   Ansar Ahmed, S ^a  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

Author keywords

Alamar Blue; Apoptosis; Cell proliferation; Cytogenetics; mRNA

Indexed keywords

COLORING AGENT; CYTOKINE; MEMBRANE ANTIGEN; MESSENGER RNA; MITOGENIC AGENT; OXAZINE DERIVATIVE; RESAZURIN; RNA; XANTHENE DERIVATIVE;

ANIMAL; APOPTOSIS; ARTICLE; BIOSYNTHESIS; C57BL MOUSE; CELL CYCLE; CELL DIVISION; CELL SURVIVAL; CHROMOSOME ABERRATION; COLORIMETRY; CYTOLOGY; DRUG EFFECT; FEMALE; FLUOROMETRY; GENETICS; IMMUNOLOGY; LYMPHOCYTE; LYMPHOCYTE ACTIVATION; MALE; METHODOLOGY; MOUSE;

ANIMALS; ANTIGENS, SURFACE; APOPTOSIS; CELL CYCLE; CELL DIVISION; CELL SURVIVAL; CHROMOSOME ABERRATIONS; COLORIMETRY; COLORING AGENTS; CYTOKINES; FEMALE; FLUOROMETRY; LYMPHOCYTE ACTIVATION; LYMPHOCYTES; MALE; MICE; MICE, INBRED C57BL; MITOGENS; OXAZINES; RNA; RNA, MESSENGER; XANTHENES;

EID: 0031573572     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00171-3     Document Type: Article

References (32)

1. Ansar Ahmed, S., Sriranganathan, N., 1994. Differential effects of dexamathasone on the thymus and spleen: alterations in programmed cell death, lymphocyte subsets and activation of T cells. Immunopharmacology 28, 55.
2. Ansar Ahmed, S., Talal, N., Christadoss, P., 1987. Genetic regulation of testosterone induced immune suppression. Cell. Immunol. 104, 91.
3. Ansar Ahmed, S., Dauphinee, M.J., Montoya, A.I., Talal, N., 1989. Estrogen induces normal murine CD5-positive B cells to produce autoantibodies. J. Immunol. 142, 2647.
4. 3H]thymidine incorporation assay. J. Immunol. Methods 170, 211.
5. Ansar Ahmed, S., Gogal, R.M. Jr., Larsen, C.T., Pierson, F.W., 1996. A novel nonradioactive diagnostic test for evaluating cell-mediated function in the chicken. Proc. XX Word's Poultry Congr. 2, 533.
6. Baker, C.N., Banerjee, S.N., Tenover, F.C., 1994. Evaluation of Alamar Colorimetric MIC for antimicrobial susceptibility testing for gram-negative bacteria. J. Clin. Microbiol. 32, 1261.
7. Espinel-Ingroff, A., Rodriguez-Tudela, J.L., Martinez-Suarez, J.V., 1995. Comparison of two alternative microdilution procedures with the national committee for clinical laboratory standards reference macrodilution method M27-P for in vitro testing of fluconazole-resistant and -susceptible isolates of Candida albicans. J. Clin. Microbiol. 33 (12), 3154.
8. de Fries, R., Masato, M., 1995. Quantification of mitogen induced human lymphocyte proliferation: Comparison of Alamar Blue™ assay to H-thymidine incorporation assay. J. Clin. Lab. Anal. 9, 89.
9. Fatima, S.K., Prabhavathi, P.A., Prasad, M.H., Padmavathi, P., Reddy, P.P., 1995. Frequencies of sister chromatic exchanges in lymphocytes of Portland Cement factory workers. Bull. Environ. Contam. Toxicol. 55 (5), 704.
10. Gogal, R.M., Jr., Ansar Ahmed, S., Larsen, C.T., 1997. Analysis of avian lymphocyte proliferation by a new, simple, non-radio-active assay (Lympho-Pro). Avian Diseases, in press.
11. Hujo, H., Tai, L.-J., Mukai, T., Hashimoto, Y., 1992. An enzyme immunoassay for cell proliferation using monoclonal antibodies against cell proliferation associated antigen. J. Pharmacobio-Dyn. 15, 567.
12. Huong, P.L.T., Kolk, A.H.J., Eggelte, T.A., Verstinjnen, C.P.H.J., Gilis, H., Hendriks, J.T., 1991. Measurement of antigen specific lymphocyte proliferation using 5-bromo-deoxyuridine incorporation. J. Immunol. Methods 140, 243.
13. Landegren, U., 1984. Measurement of cell numbers by means of endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cellular surface antigen. J. Immunol. Methods 67, 379.
14. Lefrancois, L., Lycke, N., 1996. Related isolation procedures and functional assays. In: Coligan, J.E., Kruisbeek, A.M., Margulies, D.H., Shevach, E.M., Strober, W. (Eds), Current Protocols in Immunol. John Wiley and Sons, Inc. NIH, Bethesda, MD, pp. 3.17.1-3.17.33.
15. Magaud, J.P., Sargent, I., Mason, D.Y., 1988. Detection of human white cell proliferative responses by immunoenzymatic measurement of bromodeoxyuridine uptake. J. Immunol. Methods 106, 95.
16. Moorhead, P.S., Nowell, P.C., Mellman, W.J., Battips, D.M., Hungerford, D.A., 1960. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp. Cell Res. 20, 613.
17. Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55.
18. Oliveira, S.C., Zhu, Y., Splitter, G.A., 1994. Recombinant L7/L12 ribosomal protein and g-irradiated Brucella abortus induce a T-helper 1 subset response from murine CD4+ T cells. Immunol. 83, 1.
19. Page, B., Page, M., Noel, C., 1993. A new fluorometric assay for cytotoxicity measurements in vitro. Int. J. Oncol. 3, 473.
20. Palutke, M., Kukuruga, D., Tabaczka, P., 1987. A flow cytometric method for measuring lymphocyte proliferation directly from tissue culture plates using Ki-67 and PI. J. Immunol. Method 105, 97.
21. Pfaller, M.A., Grant, C., Morthland, V., Rhine-Chalberg, J., 1994. Comparison evaluation of alternative methods for broth dilution susceptibility testing of fluconazole against Candida albicans. J. Clin. Microbiol. 32 (2), 506.
22. Porstmann, T., Ternynck, T., Avrameas, S., 1985. Quantitation of 5-bromo-2-deoxyuridice incorporation into DNA: An enzyme immunoassay for the assessment lymphoid proliferative response. J. Immunol. Method 82, 169.
23. Raza, A., Preisler, H.D., Mayers, G.L., Bankert, R., 1984. Rapid enumeration of S-phase cells by means of monoclonal antibodies. New Engl. J. Med. 310, 991.
24. Robinson, J.P., Darzynkiewicz, Z., Dean, P., Dressler, L., Tanke, H., Wheeless, L., 1993. Handbook of Flow Cytometry Methods. A John Wiley and Sons, Inc.
25. Roehm, N.W., Rodgers, G.H., Hatfield, S.M., Glasebrook, A.L., 1991. An improved colorimetric assay for cell proliferation and viability utilizing tetrazolium salt XTT. J. Immunol. Methods 142, 257.
26. Schmidt, I., Uittenbogaart, C.H., Keid, B., Giorgi, J.V., 1994. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J. Immunol. Methods 170, 145.
27. Scudiero, D.A., Shoemaker, R.H., Paull, K.D., Monks, A., Tierney, S., Nofziger, T.H., Current, M.J., Seniff, D., Boyd, M.R., 1988. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 48, 4827.
28. Squatrito, R.C., Connor, J.P., Buller, R.E., 1995. Comparison of a novel redox dye cell growth assay to the ATP bioluminescence assay. Gynecol. Oncol. 58, 101.
29. Tiballi, R.N., He, X., Zarins, L.T., Revankar, S.G., Kauffan, C.A., 1995a. Use of a colorimetric system for yeast susceptibility testing. J. Clin. Microbiol. 33 (4), 915.
30. Tiballi, R.N., Spiegel, J.E., Zarins, L.T., Kauffman, C.A., 1995b. Sacchromyces cerevisiae infections and antifungal susceptibility studies by colorimetric and broth macrodilution methods. Diagn. Microbiol. Infect. Dis. 23, 135.
31. Wemme, H., Pfeifer, S., Heck, R., Muller-Ouernheim, J., 1992. Measurement of lymphocyte proliferation: Critical analysis of radioactive and photometric methods. Immunobiology 185, 78.
32. Yajko, D.M., Madej, J.J., Lancaster, M.V., Sanders, C.A., Cawthon, V.L., Gee, B., Babst, A., Hadley, W.K., 1995. Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis. J. Clin. Microbiol. 33 (9), 2324.