A phospholipase A2 kinetic and binding assay using phospholipid-coated hydrophobic beads

Analytical Biochemistry

Volumn 250, Issue 1, 1997, Pages 109-116

  Kim, Yumee ^a   Lichtenbergova, Lenka ^a   Snitko, Yana ^a   Cho, Wonhwa ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; FATTY ACIDS; HYDROPHOBICITY; KINETICS; MAMMALS; PHOSPHOLIPIDS;

BOVINE SERUM ALBUMINS; ENZYME CONCENTRATIONS; HIGH SPECIFICITY; LINEARLY PROPORTIONAL; MEMBRANE BINDING; PHOSPHOLIPASE A2; SPECIFIC ACTIVITY; STYRENE-DIVINYLBENZENE BEADS;

STYRENE;

PHOSPHOLIPASE A2; PHOSPHOLIPID; AMBERLITE XAD 2 RESIN; AMBERLITE XAD-2 RESIN; PHOSPHOLIPASE A; POLYSTYRENE DERIVATIVE;

ARTICLE; BINDING AFFINITY; CYTOSOL; ENZYME ASSAY; ENZYME KINETICS; ENZYME SPECIFICITY; HYDROPHOBICITY; MEMBRANE BINDING; PRIORITY JOURNAL; ANIMAL; ARTIFICIAL MEMBRANE; CATTLE; CHEMISTRY; HUMAN; HYDROLYSIS; KINETICS; METABOLISM; PH; PROTEIN BINDING;

BOVINAE;

ANIMALS; CATTLE; HUMANS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MEMBRANES, ARTIFICIAL; PHOSPHOLIPASES A; PHOSPHOLIPIDS; POLYSTYRENES; PROTEIN BINDING; SUBSTRATE SPECIFICITY;

EID: 0031570794     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1997.2200     Document Type: Article

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