Recombinant strategies for rapid purification of catalytic subunits of cAMP-dependent protein kinase

Analytical Biochemistry

Volumn 245, Issue 2, 1997, Pages 115-122

  Hemmer, Wolfram ^a   McGlone, Maria ^a   Taylor, Susan S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CRYSTAL STRUCTURE; ENZYMES; RECOMBINANT PROTEINS;

ATP BINDING; CATALYTIC SUBUNITS; MOLECULAR BASIS; PROTEIN KINASE; RAPID PURIFICATION; REGULATORY SUBUNITS; SITE DIRECTED MUTAGENESIS; WILD TYPES;

PURIFICATION;

ADENOSINE TRIPHOSPHATE; CYCLIC AMP DEPENDENT PROTEIN KINASE; NICKEL; POLYHISTIDINE; RESIN;

ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME PURIFICATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECOMBINANT DNA TECHNOLOGY; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS); COLYSIS;

EID: 0031568921     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.9952     Document Type: Article

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