메뉴 건너뛰기




Volumn 266, Issue 1, 1997, Pages 93-107

The yeast site-specific recombinase Flp mediates alcoholysis and hydrolysis of the strand cleavage product: Mimicking the strand-joining reaction with non-DNA nucleophiles

Author keywords

Catalytic analysis; Flp recombinase; Hydrolysis Alcoholysis; Strand joining; Transesterification reactions

Indexed keywords

FUNGAL DNA; GLYCEROL; HYDROXYL GROUP; RECOMBINASE;

EID: 0031566951     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0767     Document Type: Article
Times cited : (19)

References (36)
  • 1
    • 49149135789 scopus 로고
    • New chemical methods for synthesizing polynuclotides
    • Beaucage S. L., Caruthers M. H. New chemical methods for synthesizing polynuclotides. Tetrahed. Letters, 22:1981;1859-1862.
    • (1981) Tetrahed. Letters , vol.22 , pp. 1859-1862
    • Beaucage, S.L.1    Caruthers, M.H.2
  • 2
    • 0026649231 scopus 로고
    • Directed evolution of an RNA enzyme
    • Beaudry A. A., Joyce G. F. Directed evolution of an RNA enzyme. Science, 257:1992;635-641.
    • (1992) Science , vol.257 , pp. 635-641
    • Beaudry, A.A.1    Joyce, G.F.2
  • 3
    • 0028598536 scopus 로고
    • Interactions of the site-specific recombinases XerC and XerD with the recombination site dif
    • Blakely G. W., Sherratt D. J. Interactions of the site-specific recombinases XerC and XerD with the recombination site dif. Nucl. Acids Res. 22:1994;5613-5620.
    • (1994) Nucl. Acids Res. , vol.22 , pp. 5613-5620
    • Blakely, G.W.1    Sherratt, D.J.2
  • 4
    • 0017757667 scopus 로고
    • 3′-Phosphatase activity in T4 polynucleotide kinase
    • Cameron V., Uhlenbeck O. 3′-Phosphatase activity in T4 polynucleotide kinase. Biochemistry. 16:1977;5120-5126.
    • (1977) Biochemistry , vol.16 , pp. 5120-5126
    • Cameron, V.1    Uhlenbeck, O.2
  • 5
    • 0026641669 scopus 로고
    • Functional analysis of Box I mutations in yeast site-specific recombinase Flp and R: Pairwise complementation with recombinase variants lacking the active-site tyrosine
    • Chen J. W., Evans B. R., Yang S. H., Araki H., Oshima Y., Jayaram M. Functional analysis of Box I mutations in yeast site-specific recombinase Flp and R: pairwise complementation with recombinase variants lacking the active-site tyrosine. Mol. Cell. Biol. 12:1992a;3757-3767.
    • (1992) Mol. Cell. Biol. , vol.12 , pp. 3757-3767
    • Chen, J.W.1    Evans, B.R.2    Yang, S.H.3    Araki, H.4    Oshima, Y.5    Jayaram, M.6
  • 6
    • 0026651559 scopus 로고
    • DNA cleavage in trans by the active site tyrosine during Flp recombination: Switching protein partners before exchanging strands
    • Chen J. W., Lee J., Jayaram M. DNA cleavage in trans by the active site tyrosine during Flp recombination: switching protein partners before exchanging strands. Cell. 69:1992b;647-658.
    • (1992) Cell , vol.69 , pp. 647-658
    • Chen, J.W.1    Lee, J.2    Jayaram, M.3
  • 7
    • 0027193305 scopus 로고
    • Tests for the fractional active-site model in Flp site-specific recombination
    • Chen J. W., Yang S. H., Jayaram M. Tests for the fractional active-site model in Flp site-specific recombination. J. Biol. Chem. 268:1993;14417-14425.
    • (1993) J. Biol. Chem. , vol.268 , pp. 14417-14425
    • Chen, J.W.1    Yang, S.H.2    Jayaram, M.3
  • 8
    • 0027211007 scopus 로고
    • Eukaryotic topoisomerase I-mediated cleavage requires bipartite DNA interaction
    • Christiansen K., Svejstrup B. D., Andersen A. H., Westergaard O. Eukaryotic topoisomerase I-mediated cleavage requires bipartite DNA interaction. J. Biol. Chem. 268:1993;9690-9701.
    • (1993) J. Biol. Chem. , vol.268 , pp. 9690-9701
    • Christiansen, K.1    Svejstrup, B.D.2    Andersen, A.H.3    Westergaard, O.4
  • 9
    • 0028279785 scopus 로고
    • The covalent eukaryotic topoisomerase I-DNA intermediate catalyzes pH-dependent hydrolysis and alcoholysis
    • Christiansen K., Knudsen B. R., Westergaard O. The covalent eukaryotic topoisomerase I-DNA intermediate catalyzes pH-dependent hydrolysis and alcoholysis. J. Biol. Chem. 269:1994;11367-11373.
    • (1994) J. Biol. Chem. , vol.269 , pp. 11367-11373
    • Christiansen, K.1    Knudsen, B.R.2    Westergaard, O.3
  • 10
    • 0028898110 scopus 로고
    • Cleavage of an amide bond by a ribozyme
    • Dai X., Mesmaeker A. D., Joyce G. F. Cleavage of an amide bond by a ribozyme. Science. 267:1995;237-239.
    • (1995) Science , vol.267 , pp. 237-239
    • Dai, X.1    Mesmaeker, A.D.2    Joyce, G.F.3
  • 11
    • 0028125987 scopus 로고
    • Mapping the functional domains of bacteriophage lambda integrase protein
    • Han Y. W., Gumport R. I., Gardner J. F. Mapping the functional domains of bacteriophage lambda integrase protein. J. Mol. Biol. 235:1994;908-925.
    • (1994) J. Mol. Biol. , vol.235 , pp. 908-925
    • Han, Y.W.1    Gumport, R.I.2    Gardner, J.F.3
  • 12
    • 0011167645 scopus 로고
    • Mechanism of site-specific recombination: The Flp paradigm
    • Berlin, Heidelberg: Springer-Verlag
    • Jayaram M. Mechanism of site-specific recombination: the Flp paradigm. Nucleic Acids and Molecular Biology. 1994;Springer-Verlag, Berlin, Heidelberg.
    • (1994) Nucleic Acids and Molecular Biology
    • Jayaram, M.1
  • 13
    • 0027277026 scopus 로고
    • Sequence-specific cleavage via nucleophilic attack of hydrogen peroxide, assisted by Flp recombinase
    • Kimball A. S., Lee J., Jayaram M., Tullius T. D. Sequence-specific cleavage via nucleophilic attack of hydrogen peroxide, assisted by Flp recombinase. Biochemistry. 32:1993;4698-4701.
    • (1993) Biochemistry , vol.32 , pp. 4698-4701
    • Kimball, A.S.1    Lee, J.2    Jayaram, M.3    Tullius, T.D.4
  • 14
    • 0027248517 scopus 로고
    • Mechanism of site-specific recombination
    • Lee J., Jayaram M. Mechanism of site-specific recombination. J. Biol. Chem. 268:1993;17564-17579.
    • (1993) J. Biol. Chem. , vol.268 , pp. 17564-17579
    • Lee, J.1    Jayaram, M.2
  • 15
    • 0029088838 scopus 로고
    • Functional roles of individual recombinase monomers in strand breakage and strand union during site-specific DNA recombinase
    • Lee J., Jayaram M. Functional roles of individual recombinase monomers in strand breakage and strand union during site-specific DNA recombinase. J. Biol. Chem. 270:1995a;23203-23211.
    • (1995) J. Biol. Chem. , vol.270 , pp. 23203-23211
    • Lee, J.1    Jayaram, M.2
  • 16
    • 0028906487 scopus 로고
    • Role of partner homology in DNA recombination. Complementary base pairing orients the 5′-hydroxyl for strand joining during Flp site-specific recombination
    • Lee J., Jayaram M. Role of partner homology in DNA recombination. Complementary base pairing orients the 5′-hydroxyl for strand joining during Flp site-specific recombination. J. Biol. Chem. 270:1995b;4042-4052.
    • (1995) J. Biol. Chem. , vol.270 , pp. 4042-4052
    • Lee, J.1    Jayaram, M.2
  • 17
    • 0024316466 scopus 로고
    • DNA topoisomerase poisons as antitumor drugs
    • Liu L. F. DNA topoisomerase poisons as antitumor drugs. Annu. Rev. Biochem. 58:1989;351-375.
    • (1989) Annu. Rev. Biochem. , vol.58 , pp. 351-375
    • Liu, L.F.1
  • 18
    • 0026799383 scopus 로고
    • Polynucleotidyl transfer reactions in transpositional DNA recombination
    • Mizuuchi K. Polynucleotidyl transfer reactions in transpositional DNA recombination. J. Biol. Chem. 267:1992;21273-21276.
    • (1992) J. Biol. Chem. , vol.267 , pp. 21273-21276
    • Mizuuchi, K.1
  • 20
    • 0026748245 scopus 로고
    • Ligation activity of Flp recombinase
    • Pan G., Sadowski P. D. Ligation activity of Flp recombinase. J. Biol. Chem. 267:1992;12397-12399.
    • (1992) J. Biol. Chem. , vol.267 , pp. 12397-12399
    • Pan, G.1    Sadowski, P.D.2
  • 21
    • 0027403931 scopus 로고
    • Ligation of synthetic activated DNA substrates by site-specific recombinase and topoisomerase I
    • Pan G., Luetke K., Juby C. D., Brousseau R., Sadowski P. Ligation of synthetic activated DNA substrates by site-specific recombinase and topoisomerase I. J. Biol. Chem. 268:1993;3683-3689.
    • (1993) J. Biol. Chem. , vol.268 , pp. 3683-3689
    • Pan, G.1    Luetke, K.2    Juby, C.D.3    Brousseau, R.4    Sadowski, P.5
  • 22
    • 0023708318 scopus 로고
    • Step-arrest mutants of Flp recombinase: Implications for the catalytic mechanism of DNA recombination
    • Parsons R. L., Prasad P. V., Harshey R. M., Jayaram M. Step-arrest mutants of Flp recombinase: implications for the catalytic mechanism of DNA recombination. Mol. Cell. Biol. 8:1988;3303-3310.
    • (1988) Mol. Cell. Biol. , vol.8 , pp. 3303-3310
    • Parsons, R.L.1    Prasad, P.V.2    Harshey, R.M.3    Jayaram, M.4
  • 23
    • 0025264936 scopus 로고
    • Functional analyses of Arg-308 mutants of Flp recombinase: Possible role of Arg-308 in coupling substrate binding to catalysis
    • Parsons R. L., Evans B. R., Zheng L., Jayaram M. Functional analyses of Arg-308 mutants of Flp recombinase: possible role of Arg-308 in coupling substrate binding to catalysis. J. Biol. Chem. 265:1990;4527-4533.
    • (1990) J. Biol. Chem. , vol.265 , pp. 4527-4533
    • Parsons, R.L.1    Evans, B.R.2    Zheng, L.3    Jayaram, M.4
  • 24
    • 0024396338 scopus 로고
    • Structure of bleomycin-induced DNA double-strand breaks: Predominance of blunt ends and single-base 5′-extensions
    • Povirk L. F., Han Y.-H., Steighner R. J. Structure of bleomycin-induced DNA double-strand breaks: predominance of blunt ends and single-base 5′-extensions. Biochemistry. 28:1989;5808-5814.
    • (1989) Biochemistry , vol.28 , pp. 5808-5814
    • Povirk, L.F.1    Han, Y.-H.2    Steighner, R.J.3
  • 25
    • 0023322410 scopus 로고
    • Mutations in the 2 microns circle site-specific recombinase that abolish recombination without affecting substrate recognition
    • Prasad P. V., Young L. J., Jayaram M. Mutations in the 2 microns circle site-specific recombinase that abolish recombination without affecting substrate recognition. Proc. Natl Acad. Sci. U.S.A. 84:1987;2189-2193.
    • (1987) Proc. Natl Acad. Sci. U.S.A. , vol.84 , pp. 2189-2193
    • Prasad, P.V.1    Young, L.J.2    Jayaram, M.3
  • 26
    • 0025649276 scopus 로고
    • Protein-based asymmetry and protein-protein interactions in Flp recombinase mediated site-specific recombination
    • Qian X.-H., Inman R. B., Cox M. M. Protein-based asymmetry and protein-protein interactions in Flp recombinase mediated site-specific recombination. J. Biol. Chem. 265:1990;21779-21788.
    • (1990) J. Biol. Chem. , vol.265 , pp. 21779-21788
    • Qian, X.-H.1    Inman, R.B.2    Cox, M.M.3
  • 28
    • 0028843052 scopus 로고
    • The Flp recombinase of the 2-microns plasmid of Saccharomyces cerevisiae
    • Sadowski P. D. The Flp recombinase of the 2-microns plasmid of Saccharomyces cerevisiae. Prog. Nucl. Acid. Res. Mol. Biol. 51:1995;53-91.
    • (1995) Prog. Nucl. Acid. Res. Mol. Biol. , vol.51 , pp. 53-91
    • Sadowski, P.D.1
  • 29
    • 0026747140 scopus 로고
    • Half-site strand transfer by step-arrest mutants of yeast site-specific recombinase Flp
    • Serre M. C., Jayaram. M. Half-site strand transfer by step-arrest mutants of yeast site-specific recombinase Flp. J. Mol. Biol. 225:1992;643-649.
    • (1992) J. Mol. Biol. , vol.225 , pp. 643-649
    • Serre, M.C.1    Jayaram, M.2
  • 30
    • 0027398023 scopus 로고
    • DNA splicing by an active site mutant of Flp recombinase
    • Serre M. C., Zheng L., Jayaram M. DNA splicing by an active site mutant of Flp recombinase. J. Biol. Chem. 268:1993;455-463.
    • (1993) J. Biol. Chem. , vol.268 , pp. 455-463
    • Serre, M.C.1    Zheng, L.2    Jayaram, M.3
  • 31
    • 0019886130 scopus 로고
    • Mechanism of replication of human mitochondrial DNA. Localization of the 5′ ends of nascent daughter strands
    • Tapper D. P., Clayton D. A. Mechanism of replication of human mitochondrial DNA. localization of the 5′ ends of nascent daughter strands. Nucl. Acids Res. 9:1981;6787-6794.
    • (1981) Nucl. Acids Res. , vol.9 , pp. 6787-6794
    • Tapper, D.P.1    Clayton, D.A.2
  • 32
    • 0026348879 scopus 로고
    • Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein
    • Vink C., Yeheskiely E., Marel G. A., Boom J. H., Plasterk R. H. A. Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein. Nucl. Acids Res. 19:1991;6691-6698.
    • (1991) Nucl. Acids Res. , vol.19 , pp. 6691-6698
    • Vink, C.1    Yeheskiely, E.2    Marel, G.A.3    Boom, J.H.4    Plasterk, R.H.A.5
  • 33
    • 0028072794 scopus 로고
    • Active-site assembly and mode of DNA cleavage by Flp recombinase during full-site recombination
    • Whang I., Lee J., Jayaram M. Active-site assembly and mode of DNA cleavage by Flp recombinase during full-site recombination. Mol. Cell. Biol. 14:1994;7492-7498.
    • (1994) Mol. Cell. Biol. , vol.14 , pp. 7492-7498
    • Whang, I.1    Lee, J.2    Jayaram, M.3
  • 35
    • 0029082452 scopus 로고
    • Cleavage-dependent ligation by the Flp recombinase. Characterization of a mutant Flp protein with an alteration in a catalytic amino acid
    • Zhu X.-D., Sadowski P. D. Cleavage-dependent ligation by the Flp recombinase. Characterization of a mutant Flp protein with an alteration in a catalytic amino acid. J. Biol. Chem. 270:1995;23044-23054.
    • (1995) J. Biol. Chem. , vol.270 , pp. 23044-23054
    • Zhu, X.-D.1    Sadowski, P.D.2
  • 36
    • 0029010662 scopus 로고
    • Homology requirements for ligation and strand exchange by the Flp recombinase
    • Zhu X.-D., Pan G., Luetke K., Sadowski P. D. Homology requirements for ligation and strand exchange by the Flp recombinase. J. Biol. Chem. 270:1995;23044-23054.
    • (1995) J. Biol. Chem. , vol.270 , pp. 23044-23054
    • Zhu, X.-D.1    Pan, G.2    Luetke, K.3    Sadowski, P.D.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.