Rat gonadotropin-releasing hormone receptor expressed in insect cells induces activation of adenylyl cyclase

Molecular and Cellular Endocrinology

Volumn 135, Issue 2, 1997, Pages 119-127

  Delahaye, Richard ^a   Manna, Pulak R ^a   Bérault, Annette ^a   Berreur Bonnenfant, Josette ^a   Berreur, Paul ^a   Counis, Raymond ^a  

^a SORBONNE UNIVERSITÉ   (France)

Author keywords

Adenylyl cyclase; Baculovirus insect cells; GnRH binding; Phosphoinositidase C; Rat GnRH receptor

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADENYLATE CYCLASE; CYCLIC AMP; GONADORELIN RECEPTOR; GONADOTROPIN; GUANINE NUCLEOTIDE BINDING PROTEIN; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE PHOSPHODIESTERASE; PHOSPHORUS 32;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; DISSOCIATION CONSTANT; DOSE TIME EFFECT RELATION; ENZYME ACTIVATION; GENE EXPRESSION; GONADOTROPIN RELEASE; HYPOPHYSIS; INSECT; NONHUMAN; PRIORITY JOURNAL; RAT; RECEPTOR GENE;

ADENYLATE CYCLASE; ANIMALS; CELL LINE; CLONE CELLS; CYCLIC AMP; ENZYME ACTIVATION; GENE EXPRESSION; INOSITOL 1,4,5-TRISPHOSPHATE; INSECTS; PROTEIN BINDING; RATS; RECEPTORS, LHRH; RECOMBINANT PROTEINS;

EID: 0031565959     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0303-7207(97)00194-9     Document Type: Article

References (50)

1. Anderson L., McGregor A., Cook J.V., Chilvers E., Eidne K.A. Rapid desensitization of GnRH-stimulated intracellular signalling events in alpha T3-1 and HEK-293 cells expressing the GnRH receptor. Endocrinology. 136:1995;5228-5231.
2. Ban E., Arias M.C., Latouche J. et al. GnRH receptors in rat brain, pituitary and testis; modulation following surgical and gonadotropin-releasing hormone agonist-induced castration. Mol. Cell. Endocrinol. 70:1990;99-107.
3. Beckers T., Marheineke K., Reiländer H., Hilgard P. Selection and characterization of mammalian cell lines with stable over-expression of human pituitary receptors for gonadoliberin. Eur. J. Biochem. 231:1995;535-543.
4. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
5. Christophe S., Robert P., Maugain S., Bellet D., Koman A., Bidart J.M. Expression of the human follicle-stimulating hormone receptor in the baculovirus system. Biochem. Biophys. Res. Commun. 196:1993;402-408.
6. Clayton R.N., Catt K.J. Gonadotropin releasing hormone receptors: characterization, physiological regulation and relationship to reproductive function. Endocr. Rev. 2:1981;186-209.
7. Clayton, R.N., Shakespear, R.A., Duncan, J.A., Marshall, J.C., with appendix by Munson, P., Rodbard, D., 1979. Radioiodinated nondegradable gonadotropin-releasing hormone analogs: New probes for the investigation of pituitary gonadotropin-releasing hormone receptors. Endocrinology 105, 1369-1381.
8. nd ed. Raven Press, New York, pp. 1815-1832.
9. Conn P.M., Morrell D.V., Dufau M.L., Catt K.J. Gonadotropin-releasing hormone action in cultured pituicytes: Independence of luteinizing hormone release and adenosine 3′,5′-monophosphate production. Endocrinology. 104:1979;448-453.
10. Counis R., Jutisz M. Regulation of pituitary gonadotropin gene expression - Outline of intracellular signaling pathways. Trends Endocrinol. Metab. 2:1991;181-187.
11. 32]ATP as substrate. Anal. Biochem. 84:1978;179-185.
12. Davidson J.S., Flanagan C.A., Zhou W. et al. Identification of N-glycosylation sites in the gonadotropin-releasing hormone receptor: role in receptor expression but not ligand binding. Mol. Cell. Endocrinol. 107:1995;241-245.
13. Davidson J.S., Flanagan C.A., Davies P.D. et al. Incorporation of an additional glycosylation site enhances expression of functional human gonadotropin-releasing hormone receptor. Endocrine. 4:1996;207-212.
14. Delahaye R., Berreur P., Salesse R., Counis R. Insect cells infected with a recombinant baculovirus express both O- and N-glycosylated forms of the rat glycoprotein hormone alpha-subunit. J. Mol. Endocrinol. 16:1996;141-149.
15. Dohlman H.G., Thorner J., Caron M.G., Lefkowitz R.J. Model systems for the study of seven-transmembrane-segment receptors. Ann. Rev. Biochem. 60:1991;653-688.
16. Geisse S., Gram H., Kleuser B., Kocher H.P. Eukaryotic expression systems: a comparison. Protein. Express. Purif. 8:1996;271-282.
17. Gharib S.D., Wierman M.E., Shupnik M.A., Chin W.W. Molecular biology of the pituitary gonadotropins. Endocr. Rev. 11:1990;177-199.
18. Hawes B.E., Barnes S., Conn P.M. Cholera toxin and pertussis toxin provoke differential effects on luteinizing hormone release, inositol phosphate production, and gonadotropin-releasing hormone (GnRH) receptor binding in the gonadotrope: evidence for multiple guanyl nucleotide binding proteins in GnRH action. Endocrinology. 132:1993;2124-2130.
19. 11. Mol. Endocrinol. 6:1992;1673-1681.
20. Jennes L., Brame B., Centers A., Janovick J.A., Conn P.M. Regulation of hippocampal gonadotropin releasing hormone (GnRH) receptor mRNA and GnRH-stimulated inositol phosphate production by gonadal steroid hormones. Mol. Brain Res. 33:1995;104-110.
21. Kaiser U.B., Conn P.M., Chin W.W. Studies of gonadotropin-releasing hormone (GnRH) action using GnRH receptor-expressing pituitary cell lines. Endocr. Rev. 18:1997;46-70.
22. Kleymann G., Boege F., Hahn M., Hampe W., Vasudevan S., Reiländer H. Human beta 2-adrenergic receptor produced in stably transformed insect cells is functionally coupled via endogenous GTP-binding protein to adenylyl cyclase. Eur. J. Biochem. 213:1993;797-804.
23. Kosugi S., Sugawa H., Mori T. TSH receptor and LH receptor, 1996. Endocr. J. 43:1996;595-604.
24. Krsmanovic, L.Z., Mores, N., Arora, K.K., Catt, K.J., 1996. 10th International Congress of Endocrinology. San Francisco CA, June 12-15, Abstract no P1-381.
25. Kuphal D., Janovick J.A., Kaiser U.B., Chin W.W., Conn P.M. Stable transfection of GH(3) cells with rat gonadotropin-releasing hormone receptor complementary deoxyribonucleic acid results in expression of a receptor coupled to cyclic adenosine 3′,5′-monophosphate-dependent prolactin release via a G protein. Endocrinology. 135:1994;315-320.
26. Kusui T., Hellmich M.R., Wang L.H., Evans R.L., Benya R.V., Battey J.F., Jensen R.T. Characterization of gastrin-releasing peptide receptor expressed in Sf9 insect cells by baculovirus. Biochemistry. 34:1995;8061-8075.
27. Kwatra M.M., Schwinn D.A., Schreurs J. et al. The substance P receptor, which couples to Gq/11, is a substrate of β-adrenergic receptor kinase 1 and 2. J. Biol. Chem. 268:1993;9161-9164.
28. Labrecque J., Fargin A., Bouvier M., Chidiac P., Dennis M. Serotonergic antagonists differentially inhibit spontaneous activity and decrease ligand binding capacity of the rat 5-hydroxytryptamine type 2C receptor in Sf9 cells. Mol. Pharmacol. 48:1995;150-159.
29. McArdle C.A., Counis R. GnRH and PACAP action in gonadotropes-cross-talk between phosphoinositidase C and adenylyl cyclase mediated signaling pathways. Trends Endocrinol. Metab. 7:1996;168-175.
30. Misrahi M., Ghinea N., Sar S. et al. Processing of the precursors of the human thyroid-stimulating hormone receptor in various eukaryotic cells (human thyrocytes, transfected L cells and baculovirus-infected insect cells). Eur. J. Biochem. 222:1994;711-719.
31. Moumni M., Kottler M.L., Counis R. Nucleotide sequence analysis of mRNAs predicts that rat pituitary and gonadal gonadotropin-releasing hormone receptor proteins have identical primary structure. Biochem. Biophys. Res. Commun. 200:1994;1359-1366.
32. Mulheron J.G., Casanas S.J., Arthur J.M., Garnovskaya M.N., Gettys T.W., Raymond J.R. Human 5-HT1A receptor expressed in insect cells activates endogenous G(o)-like G protein(s). J. Biol. Chem. 269:1994;12954-12962.
33. Muller L., Picart R., Barret A., Bockaert J., Homburger V., Tougard C. Identification of multiple subunits of heterotrimeric G proteins on the membrane of secretory granules in rat prolactin anterior pituitary cells. Mol. Cell. Neurosci. 5:1994;556-566.
34. Narayan P., Gray J., Puett D. Expression of functional lutropin choriogonadotropin receptor in the baculovirus system. Mol. Cell. Endocrinol. 117:1996;95-100.
35. Neill J.D., Sellers J.C., Musgrove L.C., Duck L.W. Epitope-tagged gonadotropin-releasing hormone receptors heterologously-expressed in mammalian (COS-1) and insect (Sf9) cells. Mol. Cell. Endocrinol. 127:1997;143-154.
36. Ng G.Y.K., George S.R., Zastawny R.L., Caron M., Bouvier M., Dennis M., O'Dowd B.F. Human serotonin (1B) receptor expression in Sf9 cells-Phosphorylation, palmitoylation, and adenylyl cyclase inhibition. Biochemistry. 32:1993;11727-11733.
37. O'Reilly, D.R., Miller, L.K., Luckow, V.A., 1992. Baculovirus expression vectors - A laboratory manual. W.H. Freeman, New York.
38. Parker E.M., Grisel D.A., Iben L.G., Nowak H.P., Mahle C.D., Yocca F.D., Gaughan G.T. Characterization of human 5-HT1 receptors expressed in Sf9 insect cells. Eur. J. Pharmacol. (Mol. Pharmacol. Sect.). 268:1994;43-53.
39. Quehenberger O., Prossnitz E.R., Cochrane C.G., Ye R.D. Absence of Gi proteins in the Sf9 insect cell. J. Biol. Chem. 267:1992;19757-19760.
40. 1-adrenergic receptor-induced cyclic AMP formation in Sf9 cells. Biochim. Biophys. Acta. 1267:1995;1-5.
41. Sato M., Kataoka R., Dingus J., Wilcox M., Hildebrandt J.D., Lanier S.M. Factors determining specificity of signal transduction bu G protein-coupled receptor. J. Biol. Chem. 270:1995;15269-15276.
42. Schomerus E., Poch A., Bunting R., Mason W.T., McArdle C.A. Effects of pituitary adenylate cyclase-activating polypeptide in the pituitary: Activation of two signal transduction pathways in the gonadotrope-derived αT3-1 cell line. Endocrinology. 134:1994;315-323.
43. Sealfon S.C., Zhou W., Almaula N., Rodic V. Cloning and site-directed mutagenesis studies of gonadotropin-releasing hormone receptor. Methods Neurosci. 29:1996;143-169.
44. Sim P.J., Wolbers W.B., Mitchell R. Activation of MAP kinase by the LHRH receptor through a dual mechanism involving protein kinase C and a pertussis toxin-sensitive G protein. Mol. Cell. Endocrinol. 112:1995;257-263.
45. Spiegel A.M., Shenker A., Weinstein L.S. Receptor-effector coupling by G proteins: Implication for normal and abnormal signal transduction. Endocr. Rev. 13:1992;536-565.
46. Stojilkovic S.S., Krsmanovic L.Z., Spergel D.J., Catt K.J. Gonadotropin-releasing hormone neurons-Intrinsic pulsatility and receptor-mediated regulation. Trends Endocrinol. Metab. 5:1994;201-209.
47. Summers, M.D., Smith, G.E., 1987. A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin, 1555.
48. Théoleyre M., Bérault A., Garnier J., Jutisz M. Binding of gonadotropin-releasing hormone (LH-RH) to the pituitary plasma membranes and the problem of adenylate cyclase stimulation. Mol. Cell. Endocrinol. 5:1976;365-377.
49. Tournier C., Gavaret J.M., Jacquemin C., Pierre M., Saunier B. Stimulation of mitogen-activated protein kinase by thyrotropin in astrocytes. Eur. J. Biochem. 228:1995;16-22.
50. Wilson B.S., Komuro M., Farquhar M.G. Cellular variations in heterotrimeric G protein localization and expression in rat pituitary. Endocrinology. 134:1994;233-244.