Low concentrations of the phosphatase inhibitor okadaic acid stop tumor cell locomotion

European Journal of Pharmacology

Volumn 324, Issue 1, 1997, Pages 99-108

  Niggli, Verena ^a   Keller, Hansuli ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

Calyculin A; Okadaic acid; Phosphatase; Tumor cell locomotion; Vimentin; Walker 256 carcinosarcoma cell

Indexed keywords

CALYCULIN A; OKADAIC ACID; PHOSPHATASE; VIMENTIN;

ARTICLE; CELL MIGRATION; CELL POLARITY; CELL SHAPE; CONTROLLED STUDY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; TUMOR CELL; WALKER CARCINOMA;

ACTINS; ANIMALS; CARCINOMA 256, WALKER; CELL MOVEMENT; CELL SIZE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG INTERACTIONS; ENZYME INHIBITORS; IMMUNOBLOTTING; OKADAIC ACID; OXAZOLES; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PROTEIN KINASE C; STAINING AND LABELING; TUMOR CELLS, CULTURED; VIMENTIN;

EID: 0031564628     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(97)00056-3     Document Type: Article

References (29)

1. Blankson, H., I. Holen and P.O. Seglen, 1995, Disruption of the cytokeratin cytoskeleton and inhibition of hepatocytic autophagy by okadaic acid, Exp. Cell Res. 218, 522.
2. Chartier, L., L.L. Rankin, R.E. Allen, Y. Kato, N. Fusetani, H. Karaki, S. Watanabe and D.J. Hartshorne, 1991, Calyculin A increases the level of protein phosphorylation and changes the shape of 3T3 fibroblasts, Cell Motil. Cytoskel. 18, 26.
3. Cohen, P., C.F.B. Holmes and Y. Tsukitani, 1990, Okadaic acid; a new probe for the study of cellular regulation, Trends Biochem. Sci. 15, 98.
4. Cunningham, C.C., 1995, Actin polymerization and intracellular solvent flow in cell surface blebbing, J. Cell Biol. 129, 1589.
5. Dunbar, B.S., H. Kimura and T.M. Timmons, 1990, Protein analysis using high resolution two-dimensional polyacrylamide gel electrophoresis, Methods Enzymol. 182, 441.
6. Evans, R.M., 1989, Phosphorylation of vimentin in mitotically selected cells. In vitro cyclic AMP-independent kinase and calcium-stimulated phosphatase activities, J. Cell Biol. 108, 67.
7. Hirano, K. and D.J. Hartshorne, 1993, Phosphorylation of vimentin in the C-terminal domain after exposure to calyculin A, Eur. J. Cell Biol. 62, 59.
8. Hirano, K., L. Chartier, R.G. Taylor, R.E. Allen, N. Fusetani, H. Karaki and D.J. Hartshorne, 1992, Changes in the cytoskeleton of 3T3 fibroblasts induced by the phosphatase inhibitor, calyculin A, J. Musc. Res. Cell Motil. 13, 341.
9. Inagaki, M., Y. Matsuoka, K. Tsujimura, S. Ando, T. Tokui, T. Takahashi and N. Inagaki, 1996, Dynamic property of intermediate filaments: regulation by phosphorylation, BioEssays 18, 481.
10. Ishihara, H., B.L. Martin, D.L. Brautigan, H. Karaki, H. Ozaki, Y. Kato, N. Fusetani, S. Watabe, K. Hashimoto, D. Uemura and D.J. Hartshorne, 1989, Calyculin A and okadaic acid: inhibitors of protein phosphatase activity, Biochem. Biophys. Res. Commun. 159, 871.
11. Keller, H.U. and A. Zimmermann, 1986, Shape changes and chemokinesis of Walker 256 carcinosarcoma cells in responses to colchicine, vinblastine, nocodazole and taxol, Inv. Metastasis 6, 33.
12. Keller, H.U., A. Zimmermann and H. Cottier, 1985, Phorbol myristate acetate (PMA) suppresses polarization and locomotion and alters F-actin content of Walker carcinosarcoma cells, Int. J. Cancer 36, 495.
13. Keller, H.U., A. Zimmermann and V. Niggli, 1989, Diacylglycerols and the protein kinase inhibitor H-7 suppress cell polarity and locomotion of Walker 256 carcinosarcoma cells, Int. J. Cancer 44, 934.
14. Kreienbühl, P., H.U. Keller and V. Niggli, 1992, Protein phosphatase inhibitors okadaid acid and calyculin A alter cell shape and F-actin distribution and inhibit stimulus-dependent increases in cytoskeletal actin of human neutrophils, Blood 80, 2911.
15. Kuhn, O. and F.H. Wilt, 1980, Double labeling of chromatin proteins, in vivo and in vitro, and their two-dimensional electrophoretic resolution, Anal. Biochem. 105, 274.
16. Laemmli, U.K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680.
17. Lee, W.C., J.S. Yu, S.D. Yang and Y.K. Lai, 1992, Reversible hyperphosphorylation and reorganization of vimentin intermediate filaments by okadaic acid in 9L rat brain tumor cells, J. Cell. Biochem. 49, 378.
18. Liotta, L.A., P.S. Steeg and W.G. Stetler-Stevenson, 1991, Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation, Cell 64, 327.
19. Mahadevan, L.C, A.C. Willis and M.J. Barrat, 1991, Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors, Cell 65, 775.
20. Maier, G.D., M.A. Wright, Y. Lozano, A. Djordjevic, J.P. Matthews and M.R. Young, 1995, I. Regulation of cytoskeletal organization in tumor cells by protein phosphatases-1 and -2A, Int. J. Cancer 61, 54.
21. Meyer, T., U. Regenass, D. Fabbro, E. Alteri, J. Rösel, M. Müller, G. Caravatti and A. Matter, 1989, A derivative of staurosporine (CGP 41 251) shows selectivity for protein kinase C inhibition and in vitro antiproliferative as well as in vivo anti-tumor activity, Int. J. Cancer 43, 851.
22. Niggli, V., A. Zimmermann and H.U. Keller, 1996, Inhibition of protein kinase C-dependent protein phosphorylation correlates with increased polarity and locomotion in Walker carcinosarcoma cells, Int. J. Cancer 65, 473.
23. Osborn, M., 1993, Vimentin, in: Guidebook to Cytoskeletal Motor Proteins, eds. Th. Kreis and R. Vale (Oxford University Press, Oxford) p. 169.
24. Schönthal, A., 1992, Okadaic acid - a valuable new tool for the study of signal transduction and cell cycle regulation? New Biol. 4, 16.
25. Twomey, B., R.E. Muid, J.S. Nixon, A.D. Sedwick, S.E. Wilkinson and M.M. Dale, 1990, The effect of new potent selective inhibitors of protein kinase C on the neutrophil respiratory burst, Biochem. Biophys. Res. Commun. 171, 1087.
26. Wilson, A.K., A. Takai, J.C. Ruegg and P. De Lanerolle, 1991, Okadaic acid, a phosphatase inhibitor, decreases macrophage motility, Am. J. Physiol. 260, L105.
27. Zheng, B., C.F. Woo and J.F. Kuo, 1991, Mitotic arrest and enhanced nuclear protein phosphorylation in human leukemia K 562 cells by okadaic acid, a potent protein phosphatase inhibitor and tumor promotor, J. Biol. Chem. 266, 10031.
28. Zimmermann, A. and H.U. Keller, 1992, Effects of staurosporin, K 252a and other structurally related protein kinase inhibitors on shape and locomotion of Walker carcinosarcoma cells, Br. J. Cancer 66, 1077.
29. Zimmermann, A. and H.U. Keller, 1993, Shape changes and chemokinesis of Walker carcino-sarcoma cells: effects of protein kinase inhibitors (HA-1004, polymixin B, sangivamycin and tamoxifen) and an inhibitor of diacylglycerol kinase (R 59022), Anticancer Res. 13, 347.