Calcium- and myristoyl-dependent subcellular localization of the neuronal calcium-binding protein VILIP in transfected PC12 cells

Neuroscience Letters

Volumn 225, Issue 2, 1997, Pages 126-128

  Spilker, Christina ^a   Gundelfinger, Eckart D ^a   Braunewell, Karl Heinz ^a  

^a LEIBNIZ INSTITUTE FOR NEUROBIOLOGY   (Germany)

Author keywords

Calcium binding protein; Calcium myristoyl switch; Cell transfection; Myristoyl mutant; PC12; Subcellular localization

Indexed keywords

CALCIUM BINDING PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; IMMUNOCYTOCHEMISTRY; MYRISTYLATION; NONHUMAN; PHEOCHROMOCYTOMA; PRIORITY JOURNAL; RAT;

ANIMALS; CALCIUM; CALCIUM-BINDING PROTEINS; IMMUNOHISTOCHEMISTRY; NERVE TISSUE PROTEINS; NEUROCALCIN; NEURONS; PC12 CELLS; RATS; RECEPTORS, CALCIUM-SENSING; TRANSFECTION;

EID: 0031552376     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(97)00201-2     Document Type: Article

References (12)

1. Braunewell, K.-H., Lenz, S.E. and Gundelfinger, E.D., VILIP (visinin-like protein). In M.R. Celio (Ed.), Guidebook to the Calcium-Binding Proteins, Oxford University Press, Oxford, UK, 1996, pp. 107-109.
2. Braunewell, K.-H., Spilker, C., Behnisch, T. and Gundelfinger, E.D., The neuronal calcium-sensor protein VILIP modulates cAMP accumulation in stably transfected C6 glioma cells: amino-terminal myristoylation determines functional activity, J. Neurochem., (1997) in press.
3. Dizhoor A.M., Chen C.-K., Olshevskaya E., Sinelnikova V.V., Phillipov P., Hurley J.B. Role of the acylated amino terminus of recoverin in Ca2+-dependent membrane interaction. Science. 259:1993;829-832.
4. Kobayashi M., Takamatsu K., Saitoh S., Nogushi T. Myristoylation of hippocalcin is linked to its membrane association properties. J. Biol. Chem. 268:1993;18898-18904.
5. Ladant D. Calcium and membrane binding properties of bovine neurocalcin delta expressed in Escherichia coli. J. Biol. Chem. 270:1995;3179-3185.
6. Lenz S.E., Henschel Y., Zopf D., Voss B., Gundelfinger E.D. VILIP, a cognate protein of the retinal calcium-binding proteins visinin and recoverin, is expressed in the developing chicken brain. Mol. Brain Res. 15:1992;133-140.
7. Lenz S.E., Braunewell K.-H., Weise C., Nedlina-Chittka A., Gundelfinger E.D. The neuronal EF-hand calcium-binding protein VILIP: interaction with cell membrane and actin-based cytoskeleton. Biochem. Biophys. Res. Commun. 225:1996;1078-1083.
8. McLaughlin S., Aderem A. The myristoyl-electrostatic switch: a modulator of reversible protein-membrane interactions. Trends Biochem. Sci. 20:1995;272-276.
9. Tanaka T., Ames J.B., Harvey T.S., Stryer L., Ikura M. Sequestration of the membrane-targeting myristoyl group of recoverin in the calcium-free state. Nature. 376:1995;444-447.
10. Towler D.A., Gordon J.I., Adams S.P., Glaser L. The biology and enzymology of eukaryotic protein acylation. Annu. Rev. Biochem. 57:1988;69-99.
11. Swierczynski S.L., Blackshear P.J. Membrane association of the myristoylated alanine-rich C kinase substrate (MARCKS) protein. J. Biol. Chem. 270:1995;13436-13445.
12. Zozulya S., Stryer L. Calcium-myristoyl protein switch, Proc. Natl. Acad. Sci. USA. 89:1992;11569-11573.